Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells

Abstract Survival of glioblastoma patients varies and prognostic markers are important in the clinical setting. With digital pathology and improved immunohistochemical multiplexing becoming a part of daily diagnostics, we investigated the prognostic value of the Ki-67 labelling index (LI) in gliobla...

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Autores principales:	Rikke H. Dahlrot, Julie A. Bangsø, Jeanette K. Petersen, Ann Mari Rosager, Mia D. Sørensen, Guido Reifenberger, Steinbjørn Hansen, Bjarne W. Kristensen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e9d2b1e38f8143a7b7f811815123cf89
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spelling	oai:doaj.org-article:e9d2b1e38f8143a7b7f811815123cf892021-12-02T17:41:12ZPrognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells10.1038/s41598-021-95958-92045-2322https://doaj.org/article/e9d2b1e38f8143a7b7f811815123cf892021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95958-9https://doaj.org/toc/2045-2322Abstract Survival of glioblastoma patients varies and prognostic markers are important in the clinical setting. With digital pathology and improved immunohistochemical multiplexing becoming a part of daily diagnostics, we investigated the prognostic value of the Ki-67 labelling index (LI) in glioblastomas more precisely than previously by excluding proliferation in non-tumor cells from the analysis. We investigated the Ki-67 LI in a well-annotated population-based glioblastoma patient cohort (178 IDH-wildtype, 3 IDH-mutated). Ki-67 was identified in full tumor sections with automated digital image analysis and the contribution from non-tumor cells was excluded using quantitative double-immunohistochemistry. For comparison of the Ki-67 LI between WHO grades (II-IV), 9 IDH-mutated diffuse astrocytomas and 9 IDH-mutated anaplastic astrocytomas were stained. Median Ki-67 LI increased with increasing WHO grade (median 2.7%, 6.4% and 27.5%). There was no difference in median Ki-67 LI between IDH-mutated and IDH-wildtype glioblastomas (p = 0.9) and Ki-67 LI was not associated with survival in glioblastomas in neither univariate (p = 0.9) nor multivariate analysis including MGMT promoter methylation status and excluding IDH-mutated glioblastomas (p = 0.2). Ki-67 may be of value in the differential diagnostic setting, but it must not be over-interpreted in the clinico-pathological context.Rikke H. DahlrotJulie A. BangsøJeanette K. PetersenAnn Mari RosagerMia D. SørensenGuido ReifenbergerSteinbjørn HansenBjarne W. KristensenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rikke H. Dahlrot Julie A. Bangsø Jeanette K. Petersen Ann Mari Rosager Mia D. Sørensen Guido Reifenberger Steinbjørn Hansen Bjarne W. Kristensen Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells
description	Abstract Survival of glioblastoma patients varies and prognostic markers are important in the clinical setting. With digital pathology and improved immunohistochemical multiplexing becoming a part of daily diagnostics, we investigated the prognostic value of the Ki-67 labelling index (LI) in glioblastomas more precisely than previously by excluding proliferation in non-tumor cells from the analysis. We investigated the Ki-67 LI in a well-annotated population-based glioblastoma patient cohort (178 IDH-wildtype, 3 IDH-mutated). Ki-67 was identified in full tumor sections with automated digital image analysis and the contribution from non-tumor cells was excluded using quantitative double-immunohistochemistry. For comparison of the Ki-67 LI between WHO grades (II-IV), 9 IDH-mutated diffuse astrocytomas and 9 IDH-mutated anaplastic astrocytomas were stained. Median Ki-67 LI increased with increasing WHO grade (median 2.7%, 6.4% and 27.5%). There was no difference in median Ki-67 LI between IDH-mutated and IDH-wildtype glioblastomas (p = 0.9) and Ki-67 LI was not associated with survival in glioblastomas in neither univariate (p = 0.9) nor multivariate analysis including MGMT promoter methylation status and excluding IDH-mutated glioblastomas (p = 0.2). Ki-67 may be of value in the differential diagnostic setting, but it must not be over-interpreted in the clinico-pathological context.
format	article
author	Rikke H. Dahlrot Julie A. Bangsø Jeanette K. Petersen Ann Mari Rosager Mia D. Sørensen Guido Reifenberger Steinbjørn Hansen Bjarne W. Kristensen
author_facet	Rikke H. Dahlrot Julie A. Bangsø Jeanette K. Petersen Ann Mari Rosager Mia D. Sørensen Guido Reifenberger Steinbjørn Hansen Bjarne W. Kristensen
author_sort	Rikke H. Dahlrot
title	Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells
title_short	Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells
title_full	Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells
title_fullStr	Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells
title_full_unstemmed	Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells
title_sort	prognostic role of ki-67 in glioblastomas excluding contribution from non-neoplastic cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e9d2b1e38f8143a7b7f811815123cf89
work_keys_str_mv	AT rikkehdahlrot prognosticroleofki67inglioblastomasexcludingcontributionfromnonneoplasticcells AT julieabangsø prognosticroleofki67inglioblastomasexcludingcontributionfromnonneoplasticcells AT jeanettekpetersen prognosticroleofki67inglioblastomasexcludingcontributionfromnonneoplasticcells AT annmarirosager prognosticroleofki67inglioblastomasexcludingcontributionfromnonneoplasticcells AT miadsørensen prognosticroleofki67inglioblastomasexcludingcontributionfromnonneoplasticcells AT guidoreifenberger prognosticroleofki67inglioblastomasexcludingcontributionfromnonneoplasticcells AT steinbjørnhansen prognosticroleofki67inglioblastomasexcludingcontributionfromnonneoplasticcells AT bjarnewkristensen prognosticroleofki67inglioblastomasexcludingcontributionfromnonneoplasticcells
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Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells

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