Identification of Bacillus strains for biological control of catfish pathogens.

Bacillus strains isolated from soil or channel catfish intestine were screened for their antagonism against Edwardsiella ictaluri and Aeromonas hydrophila, the causative agents of enteric septicemia of catfish (ESC) and motile aeromonad septicaemia (MAS), respectively. Twenty one strains were select...

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Autores principales:	Chao Ran, Abel Carrias, Malachi A Williams, Nancy Capps, Bui C T Dan, Joseph C Newton, Joseph W Kloepper, Ei L Ooi, Craig L Browdy, Jeffery S Terhune, Mark R Liles
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e9d2c3ad7e2c4568988baaa908fe5807
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spelling	oai:doaj.org-article:e9d2c3ad7e2c4568988baaa908fe58072021-11-18T07:04:30ZIdentification of Bacillus strains for biological control of catfish pathogens.1932-620310.1371/journal.pone.0045793https://doaj.org/article/e9d2c3ad7e2c4568988baaa908fe58072012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23029244/?tool=EBIhttps://doaj.org/toc/1932-6203Bacillus strains isolated from soil or channel catfish intestine were screened for their antagonism against Edwardsiella ictaluri and Aeromonas hydrophila, the causative agents of enteric septicemia of catfish (ESC) and motile aeromonad septicaemia (MAS), respectively. Twenty one strains were selected and their antagonistic activity against other aquatic pathogens was also tested. Each of the top 21 strains expressed antagonistic activity against multiple aquatic bacterial pathogens including Edwardsiella tarda, Streptococcus iniae, Yersinia ruckeri, Flavobacterium columnare, and/or the oomycete Saprolegnia ferax. Survival of the 21 Bacillus strains in the intestine of catfish was determined as Bacillus CFU/g of intestinal tissue of catfish after feeding Bacillus spore-supplemented feed for seven days followed by normal feed for three days. Five Bacillus strains that showed good antimicrobial activity and intestinal survival were incorporated into feed in spore form at a dose of 8×10(7) CFU/g and fed to channel catfish for 14 days before they were challenged by E. ictaluri in replicate. Two Bacillus subtilis strains conferred significant benefit in reducing catfish mortality (P<0.05). A similar challenge experiment conducted in Vietnam with four of the five Bacillus strains also showed protective effects against E. ictaluri in striped catfish. Safety of the four strains exhibiting the strongest biological control in vivo was also investigated in terms of whether the strains contain plasmids or express resistance to clinically important antibiotics. The Bacillus strains identified from this study have good potential to mediate disease control as probiotic feed additives for catfish aquaculture.Chao RanAbel CarriasMalachi A WilliamsNancy CappsBui C T DanJoseph C NewtonJoseph W KloepperEi L OoiCraig L BrowdyJeffery S TerhuneMark R LilesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e45793 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chao Ran Abel Carrias Malachi A Williams Nancy Capps Bui C T Dan Joseph C Newton Joseph W Kloepper Ei L Ooi Craig L Browdy Jeffery S Terhune Mark R Liles Identification of Bacillus strains for biological control of catfish pathogens.
description	Bacillus strains isolated from soil or channel catfish intestine were screened for their antagonism against Edwardsiella ictaluri and Aeromonas hydrophila, the causative agents of enteric septicemia of catfish (ESC) and motile aeromonad septicaemia (MAS), respectively. Twenty one strains were selected and their antagonistic activity against other aquatic pathogens was also tested. Each of the top 21 strains expressed antagonistic activity against multiple aquatic bacterial pathogens including Edwardsiella tarda, Streptococcus iniae, Yersinia ruckeri, Flavobacterium columnare, and/or the oomycete Saprolegnia ferax. Survival of the 21 Bacillus strains in the intestine of catfish was determined as Bacillus CFU/g of intestinal tissue of catfish after feeding Bacillus spore-supplemented feed for seven days followed by normal feed for three days. Five Bacillus strains that showed good antimicrobial activity and intestinal survival were incorporated into feed in spore form at a dose of 8×10(7) CFU/g and fed to channel catfish for 14 days before they were challenged by E. ictaluri in replicate. Two Bacillus subtilis strains conferred significant benefit in reducing catfish mortality (P<0.05). A similar challenge experiment conducted in Vietnam with four of the five Bacillus strains also showed protective effects against E. ictaluri in striped catfish. Safety of the four strains exhibiting the strongest biological control in vivo was also investigated in terms of whether the strains contain plasmids or express resistance to clinically important antibiotics. The Bacillus strains identified from this study have good potential to mediate disease control as probiotic feed additives for catfish aquaculture.
format	article
author	Chao Ran Abel Carrias Malachi A Williams Nancy Capps Bui C T Dan Joseph C Newton Joseph W Kloepper Ei L Ooi Craig L Browdy Jeffery S Terhune Mark R Liles
author_facet	Chao Ran Abel Carrias Malachi A Williams Nancy Capps Bui C T Dan Joseph C Newton Joseph W Kloepper Ei L Ooi Craig L Browdy Jeffery S Terhune Mark R Liles
author_sort	Chao Ran
title	Identification of Bacillus strains for biological control of catfish pathogens.
title_short	Identification of Bacillus strains for biological control of catfish pathogens.
title_full	Identification of Bacillus strains for biological control of catfish pathogens.
title_fullStr	Identification of Bacillus strains for biological control of catfish pathogens.
title_full_unstemmed	Identification of Bacillus strains for biological control of catfish pathogens.
title_sort	identification of bacillus strains for biological control of catfish pathogens.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e9d2c3ad7e2c4568988baaa908fe5807
work_keys_str_mv	AT chaoran identificationofbacillusstrainsforbiologicalcontrolofcatfishpathogens AT abelcarrias identificationofbacillusstrainsforbiologicalcontrolofcatfishpathogens AT malachiawilliams identificationofbacillusstrainsforbiologicalcontrolofcatfishpathogens AT nancycapps identificationofbacillusstrainsforbiologicalcontrolofcatfishpathogens AT buictdan identificationofbacillusstrainsforbiologicalcontrolofcatfishpathogens AT josephcnewton identificationofbacillusstrainsforbiologicalcontrolofcatfishpathogens AT josephwkloepper identificationofbacillusstrainsforbiologicalcontrolofcatfishpathogens AT eilooi identificationofbacillusstrainsforbiologicalcontrolofcatfishpathogens AT craiglbrowdy identificationofbacillusstrainsforbiologicalcontrolofcatfishpathogens AT jefferysterhune identificationofbacillusstrainsforbiologicalcontrolofcatfishpathogens AT markrliles identificationofbacillusstrainsforbiologicalcontrolofcatfishpathogens
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Identification of Bacillus strains for biological control of catfish pathogens.

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