Mechanotransduction channel Piezo is widely expressed in the spider, Cupiennius salei, mechanosensory neurons and central nervous system

Abstract Mechanosensory neurons use mechanotransduction (MET) ion channels to detect mechanical forces and displacements. Proteins that function as MET channels have appeared multiple times during evolution and occur in at least four different families: the DEG/ENaC and TRP channels, as well as the...

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Autores principales:	Jessica A. G. Johnson, Hongxia Liu, Ulli Höger, Samantha M. Rogers, Kajanan Sivapalan, Andrew S. French, Päivi H. Torkkeli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e9d7b9b9be864f3c9584d1964f7455b4
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spelling	oai:doaj.org-article:e9d7b9b9be864f3c9584d1964f7455b42021-12-02T18:03:07ZMechanotransduction channel Piezo is widely expressed in the spider, Cupiennius salei, mechanosensory neurons and central nervous system10.1038/s41598-021-87202-12045-2322https://doaj.org/article/e9d7b9b9be864f3c9584d1964f7455b42021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87202-1https://doaj.org/toc/2045-2322Abstract Mechanosensory neurons use mechanotransduction (MET) ion channels to detect mechanical forces and displacements. Proteins that function as MET channels have appeared multiple times during evolution and occur in at least four different families: the DEG/ENaC and TRP channels, as well as the TMC and Piezo proteins. We found twelve putative members of MET channel families in two spider transcriptomes, but detected only one, the Piezo protein, by in situ hybridization in their mechanosensory neurons. In contrast, probes for orthologs of TRP, ENaC or TMC genes that code MET channels in other species did not produce any signals in these cells. An antibody against C. salei Piezo detected the protein in all parts of their mechanosensory cells and in many neurons of the CNS. Unspecific blockers of MET channels, Ruthenium Red and GsMTx4, had no effect on the mechanically activated currents of the mechanosensory VS-3 neurons, but the latter toxin reduced action potential firing when these cells were stimulated electrically. The Piezo protein is expressed throughout the spider nervous system including the mechanosensory neurons. It is possible that it contributes to mechanosensory transduction in spider mechanosensilla, but it must have other functions in peripheral and central neurons.Jessica A. G. JohnsonHongxia LiuUlli HögerSamantha M. RogersKajanan SivapalanAndrew S. FrenchPäivi H. TorkkeliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jessica A. G. Johnson Hongxia Liu Ulli Höger Samantha M. Rogers Kajanan Sivapalan Andrew S. French Päivi H. Torkkeli Mechanotransduction channel Piezo is widely expressed in the spider, Cupiennius salei, mechanosensory neurons and central nervous system
description	Abstract Mechanosensory neurons use mechanotransduction (MET) ion channels to detect mechanical forces and displacements. Proteins that function as MET channels have appeared multiple times during evolution and occur in at least four different families: the DEG/ENaC and TRP channels, as well as the TMC and Piezo proteins. We found twelve putative members of MET channel families in two spider transcriptomes, but detected only one, the Piezo protein, by in situ hybridization in their mechanosensory neurons. In contrast, probes for orthologs of TRP, ENaC or TMC genes that code MET channels in other species did not produce any signals in these cells. An antibody against C. salei Piezo detected the protein in all parts of their mechanosensory cells and in many neurons of the CNS. Unspecific blockers of MET channels, Ruthenium Red and GsMTx4, had no effect on the mechanically activated currents of the mechanosensory VS-3 neurons, but the latter toxin reduced action potential firing when these cells were stimulated electrically. The Piezo protein is expressed throughout the spider nervous system including the mechanosensory neurons. It is possible that it contributes to mechanosensory transduction in spider mechanosensilla, but it must have other functions in peripheral and central neurons.
format	article
author	Jessica A. G. Johnson Hongxia Liu Ulli Höger Samantha M. Rogers Kajanan Sivapalan Andrew S. French Päivi H. Torkkeli
author_facet	Jessica A. G. Johnson Hongxia Liu Ulli Höger Samantha M. Rogers Kajanan Sivapalan Andrew S. French Päivi H. Torkkeli
author_sort	Jessica A. G. Johnson
title	Mechanotransduction channel Piezo is widely expressed in the spider, Cupiennius salei, mechanosensory neurons and central nervous system
title_short	Mechanotransduction channel Piezo is widely expressed in the spider, Cupiennius salei, mechanosensory neurons and central nervous system
title_full	Mechanotransduction channel Piezo is widely expressed in the spider, Cupiennius salei, mechanosensory neurons and central nervous system
title_fullStr	Mechanotransduction channel Piezo is widely expressed in the spider, Cupiennius salei, mechanosensory neurons and central nervous system
title_full_unstemmed	Mechanotransduction channel Piezo is widely expressed in the spider, Cupiennius salei, mechanosensory neurons and central nervous system
title_sort	mechanotransduction channel piezo is widely expressed in the spider, cupiennius salei, mechanosensory neurons and central nervous system
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e9d7b9b9be864f3c9584d1964f7455b4
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Mechanotransduction channel Piezo is widely expressed in the spider, Cupiennius salei, mechanosensory neurons and central nervous system

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