Synthesis, characterization, and evaluation of antibacterial effect of Ag nanoparticles against Escherichia coli O157:H7 and methicillin- resistant Staphylococcus aureus (MRSA)

Daissy Paredes,1 Claudia Ortiz,2 Rodrigo Torres1 1Escuela de Química, Facultad de Ciencias, Universidad Industrial de Santander, Colombia; 2Escuela de Bacteriología y Laboratorio Clínico, Facultad de Salud, Universidad Industrial de Santander, Colombia Abstract: Si...

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Autores principales:	Paredes D, Ortiz C, Torres R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e9dd0d36a83d4bb7be40a2116158105a
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spelling	oai:doaj.org-article:e9dd0d36a83d4bb7be40a2116158105a2021-12-02T00:35:29ZSynthesis, characterization, and evaluation of antibacterial effect of Ag nanoparticles against Escherichia coli O157:H7 and methicillin- resistant Staphylococcus aureus (MRSA)1178-2013https://doaj.org/article/e9dd0d36a83d4bb7be40a2116158105a2014-04-01T00:00:00Zhttp://www.dovepress.com/synthesis-characterization-and-evaluation-of-antibacterial-effect-of-a-a16342https://doaj.org/toc/1178-2013 Daissy Paredes,1 Claudia Ortiz,2 Rodrigo Torres1 1Escuela de Química, Facultad de Ciencias, Universidad Industrial de Santander, Colombia; 2Escuela de Bacteriología y Laboratorio Clínico, Facultad de Salud, Universidad Industrial de Santander, Colombia Abstract: Silver nanoparticles (AgNPs) have been shown great interest because of their potential antibacterial effect. Recently, this has been increased due to resistance in some pathogenic bacteria strains to conventional antibiotics, which has initiated new studies to search for more effective treatments against resistant microorganisms. For these reasons, AgNPs have become an important approach for applications in nanobiotechnology in the development of antibiotic treatment of different bacterial infections. This study was aimed at synthesizing AgNPs using cysteine as a reducer agent and cetyl-tri-methyl-ammonium bromide as a stabilizer in order to obtain more efficient treatment against the pathogen bacteria Escherichia coli O157:H7. These AgNPs were characterized through UV-Vis spectroscopy, transmission electron microscopy, and dynamic light scattering. From these analyses, formation of spherical nanoparticles with an average size of 55 nm was confirmed. Finally, minimal inhibitory concentration (MIC) and minimal bactericide concentration (MBC) of these AgNPs against pathogenic strains E. coli O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA) were determined in both solid and liquid media. MIC and MBC values were around 0.25 µg/mL and 1 µg/mL, respectively. These parameters were comparable to those reported in the literature and were even more effective than other synthesized AgNPs. Keywords: nanomaterials, antibacterial activity, minimal inhibitory concentration, MIC, minimal bactericide concentration, MBCParedes DOrtiz CTorres RDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1717-1729 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Paredes D Ortiz C Torres R Synthesis, characterization, and evaluation of antibacterial effect of Ag nanoparticles against Escherichia coli O157:H7 and methicillin- resistant Staphylococcus aureus (MRSA)
description	Daissy Paredes,1 Claudia Ortiz,2 Rodrigo Torres1 1Escuela de Química, Facultad de Ciencias, Universidad Industrial de Santander, Colombia; 2Escuela de Bacteriología y Laboratorio Clínico, Facultad de Salud, Universidad Industrial de Santander, Colombia Abstract: Silver nanoparticles (AgNPs) have been shown great interest because of their potential antibacterial effect. Recently, this has been increased due to resistance in some pathogenic bacteria strains to conventional antibiotics, which has initiated new studies to search for more effective treatments against resistant microorganisms. For these reasons, AgNPs have become an important approach for applications in nanobiotechnology in the development of antibiotic treatment of different bacterial infections. This study was aimed at synthesizing AgNPs using cysteine as a reducer agent and cetyl-tri-methyl-ammonium bromide as a stabilizer in order to obtain more efficient treatment against the pathogen bacteria Escherichia coli O157:H7. These AgNPs were characterized through UV-Vis spectroscopy, transmission electron microscopy, and dynamic light scattering. From these analyses, formation of spherical nanoparticles with an average size of 55 nm was confirmed. Finally, minimal inhibitory concentration (MIC) and minimal bactericide concentration (MBC) of these AgNPs against pathogenic strains E. coli O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA) were determined in both solid and liquid media. MIC and MBC values were around 0.25 µg/mL and 1 µg/mL, respectively. These parameters were comparable to those reported in the literature and were even more effective than other synthesized AgNPs. Keywords: nanomaterials, antibacterial activity, minimal inhibitory concentration, MIC, minimal bactericide concentration, MBC
format	article
author	Paredes D Ortiz C Torres R
author_facet	Paredes D Ortiz C Torres R
author_sort	Paredes D
title	Synthesis, characterization, and evaluation of antibacterial effect of Ag nanoparticles against Escherichia coli O157:H7 and methicillin- resistant Staphylococcus aureus (MRSA)
title_short	Synthesis, characterization, and evaluation of antibacterial effect of Ag nanoparticles against Escherichia coli O157:H7 and methicillin- resistant Staphylococcus aureus (MRSA)
title_full	Synthesis, characterization, and evaluation of antibacterial effect of Ag nanoparticles against Escherichia coli O157:H7 and methicillin- resistant Staphylococcus aureus (MRSA)
title_fullStr	Synthesis, characterization, and evaluation of antibacterial effect of Ag nanoparticles against Escherichia coli O157:H7 and methicillin- resistant Staphylococcus aureus (MRSA)
title_full_unstemmed	Synthesis, characterization, and evaluation of antibacterial effect of Ag nanoparticles against Escherichia coli O157:H7 and methicillin- resistant Staphylococcus aureus (MRSA)
title_sort	synthesis, characterization, and evaluation of antibacterial effect of ag nanoparticles against escherichia coli o157:h7 and methicillin- resistant staphylococcus aureus (mrsa)
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/e9dd0d36a83d4bb7be40a2116158105a
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Synthesis, characterization, and evaluation of antibacterial effect of Ag nanoparticles against Escherichia coli O157:H7 and methicillin- resistant Staphylococcus aureus (MRSA)

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