Jian-Pi-Zhi-Dong-Decoction regulates the expression of glutamate transporters to attenuate glutamate excitotoxicity and exerts anti-tics effects in Tourette syndrome model rats

Wenjing Yu,1,* Xiaowei Shi,2,* Xia Cui,1 Yan Niu,1 Wen Zhang,1 Xue Bai,3 Qian Wang,1 Lijun Hu,1 Sumei Wang4 1Department of Pediatrics, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, China; 2School of Acupuncture-Moxibustion and Tuin...

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Autores principales:	Yu W, Shi X, Cui X, Niu Y, Zhang W, Bai X, Wang Q, Hu L, Wang S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Jian-Pi-Zhi-Dong-Decoction Tourette syndrome Glutamate EAAT1 VGLUT1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/e9ef6f3802d942199e44d85a263eb308
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spelling	oai:doaj.org-article:e9ef6f3802d942199e44d85a263eb3082021-12-02T01:27:54ZJian-Pi-Zhi-Dong-Decoction regulates the expression of glutamate transporters to attenuate glutamate excitotoxicity and exerts anti-tics effects in Tourette syndrome model rats1178-2021https://doaj.org/article/e9ef6f3802d942199e44d85a263eb3082018-12-01T00:00:00Zhttps://www.dovepress.com/jian-pi-zhi-dong-decoction-regulates-the-expression-of-glutamate-trans-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Wenjing Yu,1,* Xiaowei Shi,2,* Xia Cui,1 Yan Niu,1 Wen Zhang,1 Xue Bai,3 Qian Wang,1 Lijun Hu,1 Sumei Wang4 1Department of Pediatrics, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, China; 2School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China; 3Department of Pediatrics, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, China; 4Department of Pediatrics, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China *These authors contributed equally to this workPurpose: This study explored whether Jian-Pi-Zhi-Dong-Decoction (JPZDD) could regulate the metabolism of glutamate (GLU) and its transporters in the striatum to exert anti-tics effects in Tourette syndrome (TS) rats.Materials and methods: We randomly assigned 56 Sprague Dawley rats into four groups, each with 14 rats: control, model, tiapride (Tia), and JPZDD. TS groups (model, Tia, and JPZDD) received intraperitoneal injection of 3,3'-iminodipropionitrile for 7 days to establish TS model. Thereafter, rats in the four groups were treated differently once a day for 6 weeks. Behavioral evaluation was performed each week by using stereotypy recording and autonomic activity test. The level of GLU in the striatum was examined by high-performance liquid chromatography. Expression of EAAT1 and VGLUT1 were measured by quantitative real-time PCR (qRT-PCR) and laser scanning confocal microscope.Results: Compared with the model group, the stereotypy score and autonomic activity were decreased in Tia and JPZDD groups. Notably, the model group had increased concentration of GLU, which decreased after JPZDD and Tia treatments. In the model group, EAAT1 and glial cells were highly co-expressed and the relative fluorescence intensity (FI) of EAAT1 was significantly lower than that in the control group. Treatment with JPZDD and Tia increased the relative FI of EAAT1. The mRNA level of EAAT1 decreased in the model group compared to that in the control group, although it was significantly elevated following JPZDD or Tia treatment. In the model group, there was low co-expression of VGLUT1 and axon cells and the FI of VGLUT1 was remarkably increased relative to that in the control group and reduced following treatment with JPZDD and Tia. A similar trend was observed in the mRNA and protein expression of VGLUT1, although it was not statistically significant.Conclusion: The mechanism by which JPZDD alleviated behavioral dysfunction of TS rats may be associated with maintaining normal GLU transport by upregulating EAAT1 and downregulating VGLUT1 in the striatum. Keywords: Jian-Pi-Zhi-Dong-Decoction, Tourette syndrome, glutamate, EAAT1, VGLUT1 Yu WShi XCui XNiu YZhang WBai XWang QHu LWang SDove Medical PressarticleJian-Pi-Zhi-Dong-DecoctionTourette syndromeGlutamateEAAT1VGLUT1Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 14, Pp 3381-3392 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Jian-Pi-Zhi-Dong-Decoction Tourette syndrome Glutamate EAAT1 VGLUT1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Jian-Pi-Zhi-Dong-Decoction Tourette syndrome Glutamate EAAT1 VGLUT1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Yu W Shi X Cui X Niu Y Zhang W Bai X Wang Q Hu L Wang S Jian-Pi-Zhi-Dong-Decoction regulates the expression of glutamate transporters to attenuate glutamate excitotoxicity and exerts anti-tics effects in Tourette syndrome model rats
description	Wenjing Yu,1,* Xiaowei Shi,2,* Xia Cui,1 Yan Niu,1 Wen Zhang,1 Xue Bai,3 Qian Wang,1 Lijun Hu,1 Sumei Wang4 1Department of Pediatrics, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, China; 2School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China; 3Department of Pediatrics, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, China; 4Department of Pediatrics, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China *These authors contributed equally to this workPurpose: This study explored whether Jian-Pi-Zhi-Dong-Decoction (JPZDD) could regulate the metabolism of glutamate (GLU) and its transporters in the striatum to exert anti-tics effects in Tourette syndrome (TS) rats.Materials and methods: We randomly assigned 56 Sprague Dawley rats into four groups, each with 14 rats: control, model, tiapride (Tia), and JPZDD. TS groups (model, Tia, and JPZDD) received intraperitoneal injection of 3,3'-iminodipropionitrile for 7 days to establish TS model. Thereafter, rats in the four groups were treated differently once a day for 6 weeks. Behavioral evaluation was performed each week by using stereotypy recording and autonomic activity test. The level of GLU in the striatum was examined by high-performance liquid chromatography. Expression of EAAT1 and VGLUT1 were measured by quantitative real-time PCR (qRT-PCR) and laser scanning confocal microscope.Results: Compared with the model group, the stereotypy score and autonomic activity were decreased in Tia and JPZDD groups. Notably, the model group had increased concentration of GLU, which decreased after JPZDD and Tia treatments. In the model group, EAAT1 and glial cells were highly co-expressed and the relative fluorescence intensity (FI) of EAAT1 was significantly lower than that in the control group. Treatment with JPZDD and Tia increased the relative FI of EAAT1. The mRNA level of EAAT1 decreased in the model group compared to that in the control group, although it was significantly elevated following JPZDD or Tia treatment. In the model group, there was low co-expression of VGLUT1 and axon cells and the FI of VGLUT1 was remarkably increased relative to that in the control group and reduced following treatment with JPZDD and Tia. A similar trend was observed in the mRNA and protein expression of VGLUT1, although it was not statistically significant.Conclusion: The mechanism by which JPZDD alleviated behavioral dysfunction of TS rats may be associated with maintaining normal GLU transport by upregulating EAAT1 and downregulating VGLUT1 in the striatum. Keywords: Jian-Pi-Zhi-Dong-Decoction, Tourette syndrome, glutamate, EAAT1, VGLUT1
format	article
author	Yu W Shi X Cui X Niu Y Zhang W Bai X Wang Q Hu L Wang S
author_facet	Yu W Shi X Cui X Niu Y Zhang W Bai X Wang Q Hu L Wang S
author_sort	Yu W
title	Jian-Pi-Zhi-Dong-Decoction regulates the expression of glutamate transporters to attenuate glutamate excitotoxicity and exerts anti-tics effects in Tourette syndrome model rats
title_short	Jian-Pi-Zhi-Dong-Decoction regulates the expression of glutamate transporters to attenuate glutamate excitotoxicity and exerts anti-tics effects in Tourette syndrome model rats
title_full	Jian-Pi-Zhi-Dong-Decoction regulates the expression of glutamate transporters to attenuate glutamate excitotoxicity and exerts anti-tics effects in Tourette syndrome model rats
title_fullStr	Jian-Pi-Zhi-Dong-Decoction regulates the expression of glutamate transporters to attenuate glutamate excitotoxicity and exerts anti-tics effects in Tourette syndrome model rats
title_full_unstemmed	Jian-Pi-Zhi-Dong-Decoction regulates the expression of glutamate transporters to attenuate glutamate excitotoxicity and exerts anti-tics effects in Tourette syndrome model rats
title_sort	jian-pi-zhi-dong-decoction regulates the expression of glutamate transporters to attenuate glutamate excitotoxicity and exerts anti-tics effects in tourette syndrome model rats
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/e9ef6f3802d942199e44d85a263eb308
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Jian-Pi-Zhi-Dong-Decoction regulates the expression of glutamate transporters to attenuate glutamate excitotoxicity and exerts anti-tics effects in Tourette syndrome model rats

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