Towards Biomanufacturing of Cell-Derived Matrices

Cell-derived matrices (CDM) are the decellularised extracellular matrices (ECM) of tissues obtained by the laboratory culture process. CDM is developed to mimic, to a certain extent, the properties of the needed natural tissue and thus to obviate the use of animals. The composition of CDM can be tai...

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Autores principales:	Weng Wan Chan, Fang Yu, Quang Bach Le, Sixun Chen, Marcus Yee, Deepak Choudhury
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cell-derived matrices extracellular matrix decellularised extracellular matrix biomaterials hydrogels biomanufacturing Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e9f023db645c48698034525e98ae8760
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id	oai:doaj.org-article:e9f023db645c48698034525e98ae8760
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spelling	oai:doaj.org-article:e9f023db645c48698034525e98ae87602021-11-11T17:20:41ZTowards Biomanufacturing of Cell-Derived Matrices10.3390/ijms2221119291422-00671661-6596https://doaj.org/article/e9f023db645c48698034525e98ae87602021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11929https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Cell-derived matrices (CDM) are the decellularised extracellular matrices (ECM) of tissues obtained by the laboratory culture process. CDM is developed to mimic, to a certain extent, the properties of the needed natural tissue and thus to obviate the use of animals. The composition of CDM can be tailored for intended applications by carefully optimising the cell sources, culturing conditions and decellularising methods. This unique advantage has inspired the increasing use of CDM for biomedical research, ranging from stem cell niches to disease modelling and regenerative medicine. However, while much effort is spent on extracting different types of CDM and exploring their utilisation, little is spent on the scale-up aspect of CDM production. The ability to scale up CDM production is essential, as the materials are due for clinical trials and regulatory approval, and in fact, this ability to scale up should be an important factor from the early stages. In this review, we first introduce the current CDM production and characterisation methods. We then describe the existing scale-up technologies for cell culture and highlight the key considerations in scaling-up CDM manufacturing. Finally, we discuss the considerations and challenges faced while converting a laboratory protocol into a full industrial process. Scaling-up CDM manufacturing is a challenging task since it may be hindered by technologies that are not yet available. The early identification of these gaps will not only quicken CDM based product development but also help drive the advancement in scale-up cell culture and ECM extraction.Weng Wan ChanFang YuQuang Bach LeSixun ChenMarcus YeeDeepak ChoudhuryMDPI AGarticlecell-derived matricesextracellular matrixdecellularised extracellular matrixbiomaterialshydrogelsbiomanufacturingBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11929, p 11929 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell-derived matrices extracellular matrix decellularised extracellular matrix biomaterials hydrogels biomanufacturing Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	cell-derived matrices extracellular matrix decellularised extracellular matrix biomaterials hydrogels biomanufacturing Biology (General) QH301-705.5 Chemistry QD1-999 Weng Wan Chan Fang Yu Quang Bach Le Sixun Chen Marcus Yee Deepak Choudhury Towards Biomanufacturing of Cell-Derived Matrices
description	Cell-derived matrices (CDM) are the decellularised extracellular matrices (ECM) of tissues obtained by the laboratory culture process. CDM is developed to mimic, to a certain extent, the properties of the needed natural tissue and thus to obviate the use of animals. The composition of CDM can be tailored for intended applications by carefully optimising the cell sources, culturing conditions and decellularising methods. This unique advantage has inspired the increasing use of CDM for biomedical research, ranging from stem cell niches to disease modelling and regenerative medicine. However, while much effort is spent on extracting different types of CDM and exploring their utilisation, little is spent on the scale-up aspect of CDM production. The ability to scale up CDM production is essential, as the materials are due for clinical trials and regulatory approval, and in fact, this ability to scale up should be an important factor from the early stages. In this review, we first introduce the current CDM production and characterisation methods. We then describe the existing scale-up technologies for cell culture and highlight the key considerations in scaling-up CDM manufacturing. Finally, we discuss the considerations and challenges faced while converting a laboratory protocol into a full industrial process. Scaling-up CDM manufacturing is a challenging task since it may be hindered by technologies that are not yet available. The early identification of these gaps will not only quicken CDM based product development but also help drive the advancement in scale-up cell culture and ECM extraction.
format	article
author	Weng Wan Chan Fang Yu Quang Bach Le Sixun Chen Marcus Yee Deepak Choudhury
author_facet	Weng Wan Chan Fang Yu Quang Bach Le Sixun Chen Marcus Yee Deepak Choudhury
author_sort	Weng Wan Chan
title	Towards Biomanufacturing of Cell-Derived Matrices
title_short	Towards Biomanufacturing of Cell-Derived Matrices
title_full	Towards Biomanufacturing of Cell-Derived Matrices
title_fullStr	Towards Biomanufacturing of Cell-Derived Matrices
title_full_unstemmed	Towards Biomanufacturing of Cell-Derived Matrices
title_sort	towards biomanufacturing of cell-derived matrices
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e9f023db645c48698034525e98ae8760
work_keys_str_mv	AT wengwanchan towardsbiomanufacturingofcellderivedmatrices AT fangyu towardsbiomanufacturingofcellderivedmatrices AT quangbachle towardsbiomanufacturingofcellderivedmatrices AT sixunchen towardsbiomanufacturingofcellderivedmatrices AT marcusyee towardsbiomanufacturingofcellderivedmatrices AT deepakchoudhury towardsbiomanufacturingofcellderivedmatrices
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Towards Biomanufacturing of Cell-Derived Matrices

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