siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants

Abstract A non-transgenic approach based on RNA interference was employed to induce protection against tomato mosaic virus (ToMV) infection in tomato plants. dsRNA molecules targeting the cp gene of ToMV were topically applied on plants prior to virus inoculation. Protection was dose-dependent and s...

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Autores principales:	Camila M. Rego-Machado, Erich Y. T. Nakasu, João M. F. Silva, Natália Lucinda, Tatsuya Nagata, Alice K. Inoue-Nagata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e9f0e902ab394634acc78df25a291199
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spelling	oai:doaj.org-article:e9f0e902ab394634acc78df25a2911992021-12-02T13:34:10ZsiRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants10.1038/s41598-020-79360-52045-2322https://doaj.org/article/e9f0e902ab394634acc78df25a2911992020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79360-5https://doaj.org/toc/2045-2322Abstract A non-transgenic approach based on RNA interference was employed to induce protection against tomato mosaic virus (ToMV) infection in tomato plants. dsRNA molecules targeting the cp gene of ToMV were topically applied on plants prior to virus inoculation. Protection was dose-dependent and sequence-specific. While no protection was achieved when 0–16 µg dsRNA were used, maximum rates of resistance (60 and 63%) were observed in doses of 200 and 400 µg/plant, respectively. Similar rates were also obtained against potato virus Y when targeting its cp gene. The protection was quickly activated upon dsRNA application and lasted for up to 4 days. In contrast, no detectable antiviral response was triggered by the dsRNA from a begomovirus genome, suggesting the method is not effective against phloem-limited DNA viruses. Deep sequencing was performed to analyze the biogenesis of siRNA populations. Although long-dsRNA remained in the treated leaves for at least 10 days, its systemic movement was not observed. Conversely, dsRNA-derived siRNA populations (mainly 21- and 22-nt) were detected in non-treated leaves, which indicates endogenous processing and transport through the plant. Altogether, this study provides critical information for the development of novel tools against plant viruses; strengths and limitations inherent to the systems are discussed.Camila M. Rego-MachadoErich Y. T. NakasuJoão M. F. SilvaNatália LucindaTatsuya NagataAlice K. Inoue-NagataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Camila M. Rego-Machado Erich Y. T. Nakasu João M. F. Silva Natália Lucinda Tatsuya Nagata Alice K. Inoue-Nagata siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants
description	Abstract A non-transgenic approach based on RNA interference was employed to induce protection against tomato mosaic virus (ToMV) infection in tomato plants. dsRNA molecules targeting the cp gene of ToMV were topically applied on plants prior to virus inoculation. Protection was dose-dependent and sequence-specific. While no protection was achieved when 0–16 µg dsRNA were used, maximum rates of resistance (60 and 63%) were observed in doses of 200 and 400 µg/plant, respectively. Similar rates were also obtained against potato virus Y when targeting its cp gene. The protection was quickly activated upon dsRNA application and lasted for up to 4 days. In contrast, no detectable antiviral response was triggered by the dsRNA from a begomovirus genome, suggesting the method is not effective against phloem-limited DNA viruses. Deep sequencing was performed to analyze the biogenesis of siRNA populations. Although long-dsRNA remained in the treated leaves for at least 10 days, its systemic movement was not observed. Conversely, dsRNA-derived siRNA populations (mainly 21- and 22-nt) were detected in non-treated leaves, which indicates endogenous processing and transport through the plant. Altogether, this study provides critical information for the development of novel tools against plant viruses; strengths and limitations inherent to the systems are discussed.
format	article
author	Camila M. Rego-Machado Erich Y. T. Nakasu João M. F. Silva Natália Lucinda Tatsuya Nagata Alice K. Inoue-Nagata
author_facet	Camila M. Rego-Machado Erich Y. T. Nakasu João M. F. Silva Natália Lucinda Tatsuya Nagata Alice K. Inoue-Nagata
author_sort	Camila M. Rego-Machado
title	siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants
title_short	siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants
title_full	siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants
title_fullStr	siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants
title_full_unstemmed	siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants
title_sort	sirna biogenesis and advances in topically applied dsrna for controlling virus infections in tomato plants
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/e9f0e902ab394634acc78df25a291199
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siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants

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