Thermal laser ablation with tunable lesion size reveals multiple origins of seizure-like convulsions in Caenorhabditis elegans

Abstract Laser microsurgery has long been an important means of assessing the functions of specific cells and tissues. Most laser ablation systems use short, highly focused laser pulses to create plasma-mediated lesions with dimensions on the order of the wavelength of light. While the small size of...

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Autores principales:	Anthony D. Fouad, Alice Liu, Angelica Du, Priya D. Bhirgoo, Christopher Fang-Yen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e9f1f99de8b645929d6cfd7aaeded008
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spelling	oai:doaj.org-article:e9f1f99de8b645929d6cfd7aaeded0082021-12-02T13:19:22ZThermal laser ablation with tunable lesion size reveals multiple origins of seizure-like convulsions in Caenorhabditis elegans10.1038/s41598-021-84516-y2045-2322https://doaj.org/article/e9f1f99de8b645929d6cfd7aaeded0082021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84516-yhttps://doaj.org/toc/2045-2322Abstract Laser microsurgery has long been an important means of assessing the functions of specific cells and tissues. Most laser ablation systems use short, highly focused laser pulses to create plasma-mediated lesions with dimensions on the order of the wavelength of light. While the small size of the lesion enables ablation with high spatial resolution, it also makes it difficult to ablate larger structures. We developed an infrared laser ablation system capable of thermally lesioning tissues with spot sizes tunable by the duration and amplitude of laser pulses. We used our laser system in the roundworm C. elegans to kill single neurons and to sever the dorsal and ventral nerve cords, structures that are difficult to lesion using a plasma-based ablation system. We used these ablations to investigate the source of convulsions in a gain-of-function mutant for the acetylcholine receptor ACR-2. Severing the ventral nerve cord caused convulsions to occur independently anterior and posterior to the lesion, suggesting that convulsions can arise independently from distinct subsets of the motor circuit.Anthony D. FouadAlice LiuAngelica DuPriya D. BhirgooChristopher Fang-YenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anthony D. Fouad Alice Liu Angelica Du Priya D. Bhirgoo Christopher Fang-Yen Thermal laser ablation with tunable lesion size reveals multiple origins of seizure-like convulsions in Caenorhabditis elegans
description	Abstract Laser microsurgery has long been an important means of assessing the functions of specific cells and tissues. Most laser ablation systems use short, highly focused laser pulses to create plasma-mediated lesions with dimensions on the order of the wavelength of light. While the small size of the lesion enables ablation with high spatial resolution, it also makes it difficult to ablate larger structures. We developed an infrared laser ablation system capable of thermally lesioning tissues with spot sizes tunable by the duration and amplitude of laser pulses. We used our laser system in the roundworm C. elegans to kill single neurons and to sever the dorsal and ventral nerve cords, structures that are difficult to lesion using a plasma-based ablation system. We used these ablations to investigate the source of convulsions in a gain-of-function mutant for the acetylcholine receptor ACR-2. Severing the ventral nerve cord caused convulsions to occur independently anterior and posterior to the lesion, suggesting that convulsions can arise independently from distinct subsets of the motor circuit.
format	article
author	Anthony D. Fouad Alice Liu Angelica Du Priya D. Bhirgoo Christopher Fang-Yen
author_facet	Anthony D. Fouad Alice Liu Angelica Du Priya D. Bhirgoo Christopher Fang-Yen
author_sort	Anthony D. Fouad
title	Thermal laser ablation with tunable lesion size reveals multiple origins of seizure-like convulsions in Caenorhabditis elegans
title_short	Thermal laser ablation with tunable lesion size reveals multiple origins of seizure-like convulsions in Caenorhabditis elegans
title_full	Thermal laser ablation with tunable lesion size reveals multiple origins of seizure-like convulsions in Caenorhabditis elegans
title_fullStr	Thermal laser ablation with tunable lesion size reveals multiple origins of seizure-like convulsions in Caenorhabditis elegans
title_full_unstemmed	Thermal laser ablation with tunable lesion size reveals multiple origins of seizure-like convulsions in Caenorhabditis elegans
title_sort	thermal laser ablation with tunable lesion size reveals multiple origins of seizure-like convulsions in caenorhabditis elegans
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e9f1f99de8b645929d6cfd7aaeded008
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Thermal laser ablation with tunable lesion size reveals multiple origins of seizure-like convulsions in Caenorhabditis elegans

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