Impact of oil on bacterial community structure in bioturbated sediments.

Oil spills threaten coastlines where biological processes supply essential ecosystem services. Therefore, it is crucial to understand how oil influences the microbial communities in sediments that play key roles in ecosystem functioning. Ecosystems such as sediments are characterized by intensive bi...

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Autores principales:	Magalie Stauffert, Cristiana Cravo-Laureau, Ronan Jézéquel, Sandra Barantal, Philippe Cuny, Franck Gilbert, Christine Cagnon, Cécile Militon, David Amouroux, Fatima Mahdaoui, Brice Bouyssiere, Georges Stora, François-Xavier Merlin, Robert Duran
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e9f8478705f64672a51418a929734bb3
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spelling	oai:doaj.org-article:e9f8478705f64672a51418a929734bb32021-11-18T07:42:29ZImpact of oil on bacterial community structure in bioturbated sediments.1932-620310.1371/journal.pone.0065347https://doaj.org/article/e9f8478705f64672a51418a929734bb32013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23762350/?tool=EBIhttps://doaj.org/toc/1932-6203Oil spills threaten coastlines where biological processes supply essential ecosystem services. Therefore, it is crucial to understand how oil influences the microbial communities in sediments that play key roles in ecosystem functioning. Ecosystems such as sediments are characterized by intensive bioturbation due to burrowing macrofauna that may modify the microbial metabolisms. It is thus essential to consider the bioturbation when determining the impact of oil on microbial communities. In this study, an experimental laboratory device maintaining pristine collected mudflat sediments in microcosms closer to true environmental conditions--with tidal cycles and natural seawater--was used to simulate an oil spill under bioturbation conditions. Different conditions were applied to the microcosms including an addition of: standardized oil (Blend Arabian Light crude oil, 25.6 mg.g⁻¹ wet sediment), the common burrowing organism Hediste (Nereis) diversicolor and both the oil and H. diversicolor. The addition of H. diversicolor and its associated bioturbation did not affect the removal of petroleum hydrocarbons. After 270 days, 60% of hydrocarbons had been removed in all microcosms irrespective of the H. diversicolor addition. However, 16S-rRNA gene and 16S-cDNA T-RFLP and RT-PCR-amplicon libraries analysis showed an effect of the condition on the bacterial community structure, composition, and dynamics, supported by PerMANOVA analysis. The 16S-cDNA libraries from microcosms where H. diversicolor was added (oiled and un-oiled) showed a marked dominance of sequences related to Gammaproteobacteria. However, in the oiled-library sequences associated to Deltaproteobacteria and Bacteroidetes were also highly represented. The 16S-cDNA libraries from oiled-microcosms (with and without H. diversicolor addition) revealed two distinct microbial communities characterized by different phylotypes associated to known hydrocarbonoclastic bacteria and dominated by Gammaproteobacteria and Deltaproteobacteria. In the oiled-microcosms, the addition of H. diversicolor reduced the phylotype-richness, sequences associated to Actinobacteria, Firmicutes and Plantomycetes were not detected. These observations highlight the influence of the bioturbation on the bacterial community structure without affecting the biodegradation capacities.Magalie StauffertCristiana Cravo-LaureauRonan JézéquelSandra BarantalPhilippe CunyFranck GilbertChristine CagnonCécile MilitonDavid AmourouxFatima MahdaouiBrice BouyssiereGeorges StoraFrançois-Xavier MerlinRobert DuranPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e65347 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Magalie Stauffert Cristiana Cravo-Laureau Ronan Jézéquel Sandra Barantal Philippe Cuny Franck Gilbert Christine Cagnon Cécile Militon David Amouroux Fatima Mahdaoui Brice Bouyssiere Georges Stora François-Xavier Merlin Robert Duran Impact of oil on bacterial community structure in bioturbated sediments.
description	Oil spills threaten coastlines where biological processes supply essential ecosystem services. Therefore, it is crucial to understand how oil influences the microbial communities in sediments that play key roles in ecosystem functioning. Ecosystems such as sediments are characterized by intensive bioturbation due to burrowing macrofauna that may modify the microbial metabolisms. It is thus essential to consider the bioturbation when determining the impact of oil on microbial communities. In this study, an experimental laboratory device maintaining pristine collected mudflat sediments in microcosms closer to true environmental conditions--with tidal cycles and natural seawater--was used to simulate an oil spill under bioturbation conditions. Different conditions were applied to the microcosms including an addition of: standardized oil (Blend Arabian Light crude oil, 25.6 mg.g⁻¹ wet sediment), the common burrowing organism Hediste (Nereis) diversicolor and both the oil and H. diversicolor. The addition of H. diversicolor and its associated bioturbation did not affect the removal of petroleum hydrocarbons. After 270 days, 60% of hydrocarbons had been removed in all microcosms irrespective of the H. diversicolor addition. However, 16S-rRNA gene and 16S-cDNA T-RFLP and RT-PCR-amplicon libraries analysis showed an effect of the condition on the bacterial community structure, composition, and dynamics, supported by PerMANOVA analysis. The 16S-cDNA libraries from microcosms where H. diversicolor was added (oiled and un-oiled) showed a marked dominance of sequences related to Gammaproteobacteria. However, in the oiled-library sequences associated to Deltaproteobacteria and Bacteroidetes were also highly represented. The 16S-cDNA libraries from oiled-microcosms (with and without H. diversicolor addition) revealed two distinct microbial communities characterized by different phylotypes associated to known hydrocarbonoclastic bacteria and dominated by Gammaproteobacteria and Deltaproteobacteria. In the oiled-microcosms, the addition of H. diversicolor reduced the phylotype-richness, sequences associated to Actinobacteria, Firmicutes and Plantomycetes were not detected. These observations highlight the influence of the bioturbation on the bacterial community structure without affecting the biodegradation capacities.
format	article
author	Magalie Stauffert Cristiana Cravo-Laureau Ronan Jézéquel Sandra Barantal Philippe Cuny Franck Gilbert Christine Cagnon Cécile Militon David Amouroux Fatima Mahdaoui Brice Bouyssiere Georges Stora François-Xavier Merlin Robert Duran
author_facet	Magalie Stauffert Cristiana Cravo-Laureau Ronan Jézéquel Sandra Barantal Philippe Cuny Franck Gilbert Christine Cagnon Cécile Militon David Amouroux Fatima Mahdaoui Brice Bouyssiere Georges Stora François-Xavier Merlin Robert Duran
author_sort	Magalie Stauffert
title	Impact of oil on bacterial community structure in bioturbated sediments.
title_short	Impact of oil on bacterial community structure in bioturbated sediments.
title_full	Impact of oil on bacterial community structure in bioturbated sediments.
title_fullStr	Impact of oil on bacterial community structure in bioturbated sediments.
title_full_unstemmed	Impact of oil on bacterial community structure in bioturbated sediments.
title_sort	impact of oil on bacterial community structure in bioturbated sediments.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e9f8478705f64672a51418a929734bb3
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Impact of oil on bacterial community structure in bioturbated sediments.

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