Adhesion molecule expression and function of primary endothelial cells in benign and malignant tissues correlates with proliferation.

<h4>Background</h4>Comparative analysis of the cellular biology of the microvasculature in different tissues requires the availability of viable primary endothelial cells (ECs). This study describes a novel method to isolate primary ECs from healthy organs, repair blastemas and tumors as...

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Autores principales:	Wolfgang Sievert, Soile Tapio, Stephanie Breuninger, Udo Gaipl, Nicolaus Andratschke, Klaus-Rüdiger Trott, Gabriele Multhoff
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ea02263b8f5a45e4b3c434a0c497f9b2
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spelling	oai:doaj.org-article:ea02263b8f5a45e4b3c434a0c497f9b22021-11-18T08:28:03ZAdhesion molecule expression and function of primary endothelial cells in benign and malignant tissues correlates with proliferation.1932-620310.1371/journal.pone.0091808https://doaj.org/article/ea02263b8f5a45e4b3c434a0c497f9b22014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24632811/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Comparative analysis of the cellular biology of the microvasculature in different tissues requires the availability of viable primary endothelial cells (ECs). This study describes a novel method to isolate primary ECs from healthy organs, repair blastemas and tumors as examples of non-proliferating and proliferating benign and malignant tissues and their functional characterization.<h4>Methodology/principal findings</h4>Single cell suspensions from hearts, lungs, repair blastemas and tumors were incubated consecutively with an anti-CD31 antibody and magnetic micro-beads, coupled to a derivative of biotin and streptavidin, respectively. Following magnetic bead separation, CD31-positive ECs were released by biotin-streptavidin competition. In the absence of micro-beads, ECs became adherent to plastic surfaces. ECs from proliferating repair blastemas and tumors were larger and exhibited higher expression densities of CD31, CD105 and CD102 compared to those from non-proliferating normal tissues such as heart and lung. The expression density of CD34 was particularly high in tumor-derived ECs, and that of CD54 and CD144 in ECs of repair blastemas. Functionally, ECs of non-proliferating and proliferating tissues differed in their capacity to form tubes in matrigel and to align under flow conditions.<h4>Conclusions/significance</h4>This method provides a powerful tool to generate high yields of viable, primary ECs of different origins. The results suggest that an altered expression of adhesion molecules on ECs in proliferating tissues contribute to loss of EC function that might cause a chaotic tumor vasculature.Wolfgang SievertSoile TapioStephanie BreuningerUdo GaiplNicolaus AndratschkeKlaus-Rüdiger TrottGabriele MulthoffPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91808 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wolfgang Sievert Soile Tapio Stephanie Breuninger Udo Gaipl Nicolaus Andratschke Klaus-Rüdiger Trott Gabriele Multhoff Adhesion molecule expression and function of primary endothelial cells in benign and malignant tissues correlates with proliferation.
description	<h4>Background</h4>Comparative analysis of the cellular biology of the microvasculature in different tissues requires the availability of viable primary endothelial cells (ECs). This study describes a novel method to isolate primary ECs from healthy organs, repair blastemas and tumors as examples of non-proliferating and proliferating benign and malignant tissues and their functional characterization.<h4>Methodology/principal findings</h4>Single cell suspensions from hearts, lungs, repair blastemas and tumors were incubated consecutively with an anti-CD31 antibody and magnetic micro-beads, coupled to a derivative of biotin and streptavidin, respectively. Following magnetic bead separation, CD31-positive ECs were released by biotin-streptavidin competition. In the absence of micro-beads, ECs became adherent to plastic surfaces. ECs from proliferating repair blastemas and tumors were larger and exhibited higher expression densities of CD31, CD105 and CD102 compared to those from non-proliferating normal tissues such as heart and lung. The expression density of CD34 was particularly high in tumor-derived ECs, and that of CD54 and CD144 in ECs of repair blastemas. Functionally, ECs of non-proliferating and proliferating tissues differed in their capacity to form tubes in matrigel and to align under flow conditions.<h4>Conclusions/significance</h4>This method provides a powerful tool to generate high yields of viable, primary ECs of different origins. The results suggest that an altered expression of adhesion molecules on ECs in proliferating tissues contribute to loss of EC function that might cause a chaotic tumor vasculature.
format	article
author	Wolfgang Sievert Soile Tapio Stephanie Breuninger Udo Gaipl Nicolaus Andratschke Klaus-Rüdiger Trott Gabriele Multhoff
author_facet	Wolfgang Sievert Soile Tapio Stephanie Breuninger Udo Gaipl Nicolaus Andratschke Klaus-Rüdiger Trott Gabriele Multhoff
author_sort	Wolfgang Sievert
title	Adhesion molecule expression and function of primary endothelial cells in benign and malignant tissues correlates with proliferation.
title_short	Adhesion molecule expression and function of primary endothelial cells in benign and malignant tissues correlates with proliferation.
title_full	Adhesion molecule expression and function of primary endothelial cells in benign and malignant tissues correlates with proliferation.
title_fullStr	Adhesion molecule expression and function of primary endothelial cells in benign and malignant tissues correlates with proliferation.
title_full_unstemmed	Adhesion molecule expression and function of primary endothelial cells in benign and malignant tissues correlates with proliferation.
title_sort	adhesion molecule expression and function of primary endothelial cells in benign and malignant tissues correlates with proliferation.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ea02263b8f5a45e4b3c434a0c497f9b2
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Adhesion molecule expression and function of primary endothelial cells in benign and malignant tissues correlates with proliferation.

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