Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI.

Ex vivo rodent lung models are explored for physiological measurements of respiratory function with hyperpolarized (hp) (129)Xe MRI. It is shown that excised lung models allow for simplification of the technical challenges involved and provide valuable physiological insights that are not feasible us...

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Autores principales:	David M L Lilburn, Theodore Hughes-Riley, Joseph S Six, Karl F Stupic, Dominick E Shaw, Galina E Pavlovskaya, Thomas Meersmann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ea02a41125144e3484287ee49e716dcc
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spelling	oai:doaj.org-article:ea02a41125144e3484287ee49e716dcc2021-11-18T08:57:32ZValidating excised rodent lungs for functional hyperpolarized xenon-129 MRI.1932-620310.1371/journal.pone.0073468https://doaj.org/article/ea02a41125144e3484287ee49e716dcc2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24023683/?tool=EBIhttps://doaj.org/toc/1932-6203Ex vivo rodent lung models are explored for physiological measurements of respiratory function with hyperpolarized (hp) (129)Xe MRI. It is shown that excised lung models allow for simplification of the technical challenges involved and provide valuable physiological insights that are not feasible using in vivo MRI protocols. A custom designed breathing apparatus enables MR images of gas distribution on increasing ventilation volumes of actively inhaled hp (129)Xe. Straightforward hp (129)Xe MRI protocols provide residual lung volume (RV) data and permit for spatially resolved tracking of small hp (129)Xe probe volumes during the inhalation cycle. Hp (129)Xe MRI of lung function in the excised organ demonstrates the persistence of post mortem airway responsiveness to intravenous methacholine challenges. The presented methodology enables physiology of lung function in health and disease without additional regulatory approval requirements and reduces the technical and logistical challenges with hp gas MRI experiments. The post mortem lung functional data can augment histological measurements and should be of interest for drug development studies.David M L LilburnTheodore Hughes-RileyJoseph S SixKarl F StupicDominick E ShawGalina E PavlovskayaThomas MeersmannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e73468 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David M L Lilburn Theodore Hughes-Riley Joseph S Six Karl F Stupic Dominick E Shaw Galina E Pavlovskaya Thomas Meersmann Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI.
description	Ex vivo rodent lung models are explored for physiological measurements of respiratory function with hyperpolarized (hp) (129)Xe MRI. It is shown that excised lung models allow for simplification of the technical challenges involved and provide valuable physiological insights that are not feasible using in vivo MRI protocols. A custom designed breathing apparatus enables MR images of gas distribution on increasing ventilation volumes of actively inhaled hp (129)Xe. Straightforward hp (129)Xe MRI protocols provide residual lung volume (RV) data and permit for spatially resolved tracking of small hp (129)Xe probe volumes during the inhalation cycle. Hp (129)Xe MRI of lung function in the excised organ demonstrates the persistence of post mortem airway responsiveness to intravenous methacholine challenges. The presented methodology enables physiology of lung function in health and disease without additional regulatory approval requirements and reduces the technical and logistical challenges with hp gas MRI experiments. The post mortem lung functional data can augment histological measurements and should be of interest for drug development studies.
format	article
author	David M L Lilburn Theodore Hughes-Riley Joseph S Six Karl F Stupic Dominick E Shaw Galina E Pavlovskaya Thomas Meersmann
author_facet	David M L Lilburn Theodore Hughes-Riley Joseph S Six Karl F Stupic Dominick E Shaw Galina E Pavlovskaya Thomas Meersmann
author_sort	David M L Lilburn
title	Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI.
title_short	Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI.
title_full	Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI.
title_fullStr	Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI.
title_full_unstemmed	Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI.
title_sort	validating excised rodent lungs for functional hyperpolarized xenon-129 mri.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ea02a41125144e3484287ee49e716dcc
work_keys_str_mv	AT davidmllilburn validatingexcisedrodentlungsforfunctionalhyperpolarizedxenon129mri AT theodorehughesriley validatingexcisedrodentlungsforfunctionalhyperpolarizedxenon129mri AT josephssix validatingexcisedrodentlungsforfunctionalhyperpolarizedxenon129mri AT karlfstupic validatingexcisedrodentlungsforfunctionalhyperpolarizedxenon129mri AT dominickeshaw validatingexcisedrodentlungsforfunctionalhyperpolarizedxenon129mri AT galinaepavlovskaya validatingexcisedrodentlungsforfunctionalhyperpolarizedxenon129mri AT thomasmeersmann validatingexcisedrodentlungsforfunctionalhyperpolarizedxenon129mri
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Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI.

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