Conjugated polymers mediate effective activation of the Mammalian Ion Channel Transient Receptor Potential Vanilloid 1

Abstract Selective and rapid regulation of ionic channels is pivotal to the understanding of physiological processes and has a crucial impact in developing novel therapeutic strategies. Transient Receptor Potential (TRP) channels are emerging as essential cellular switches that allow animals to resp...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	F. Lodola, N. Martino, G. Tullii, G. Lanzani, M. R. Antognazza
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ea056b5203764a46914a56ced95195e8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ea056b5203764a46914a56ced95195e8
record_format	dspace
spelling	oai:doaj.org-article:ea056b5203764a46914a56ced95195e82021-12-02T11:52:39ZConjugated polymers mediate effective activation of the Mammalian Ion Channel Transient Receptor Potential Vanilloid 110.1038/s41598-017-08541-62045-2322https://doaj.org/article/ea056b5203764a46914a56ced95195e82017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08541-6https://doaj.org/toc/2045-2322Abstract Selective and rapid regulation of ionic channels is pivotal to the understanding of physiological processes and has a crucial impact in developing novel therapeutic strategies. Transient Receptor Potential (TRP) channels are emerging as essential cellular switches that allow animals to respond to their environment. In particular, the Vanilloid Receptor 1 (TRPV1), besides being involved in the body temperature regulation and in the response to pain, has important roles in several neuronal functions, as cytoskeleton dynamics, injured neurons regeneration, synaptic plasticity. Currently available tools to modulate TRPV1 activity suffer from limited spatial selectivity, do not allow for temporally precise control, and are usually not reversible, thus limiting their application potential. The use of optical excitation would allow for overcoming all these limitations. Here, we propose a novel strategy, based on the use of light-sensitive, conjugated polymers. We demonstrate that illumination of a polymer thin film leads to reliable, robust and temporally precise control of TRPV1 channels. Interestingly, the activation of the channel is due to the combination of two different, locally confined effects, namely the release of thermal energy from the polymer surface and the variation of the local ionic concentration at the cell/polymer interface, both mediated by the polymer photoexcitation.F. LodolaN. MartinoG. TulliiG. LanzaniM. R. AntognazzaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q F. Lodola N. Martino G. Tullii G. Lanzani M. R. Antognazza Conjugated polymers mediate effective activation of the Mammalian Ion Channel Transient Receptor Potential Vanilloid 1
description	Abstract Selective and rapid regulation of ionic channels is pivotal to the understanding of physiological processes and has a crucial impact in developing novel therapeutic strategies. Transient Receptor Potential (TRP) channels are emerging as essential cellular switches that allow animals to respond to their environment. In particular, the Vanilloid Receptor 1 (TRPV1), besides being involved in the body temperature regulation and in the response to pain, has important roles in several neuronal functions, as cytoskeleton dynamics, injured neurons regeneration, synaptic plasticity. Currently available tools to modulate TRPV1 activity suffer from limited spatial selectivity, do not allow for temporally precise control, and are usually not reversible, thus limiting their application potential. The use of optical excitation would allow for overcoming all these limitations. Here, we propose a novel strategy, based on the use of light-sensitive, conjugated polymers. We demonstrate that illumination of a polymer thin film leads to reliable, robust and temporally precise control of TRPV1 channels. Interestingly, the activation of the channel is due to the combination of two different, locally confined effects, namely the release of thermal energy from the polymer surface and the variation of the local ionic concentration at the cell/polymer interface, both mediated by the polymer photoexcitation.
format	article
author	F. Lodola N. Martino G. Tullii G. Lanzani M. R. Antognazza
author_facet	F. Lodola N. Martino G. Tullii G. Lanzani M. R. Antognazza
author_sort	F. Lodola
title	Conjugated polymers mediate effective activation of the Mammalian Ion Channel Transient Receptor Potential Vanilloid 1
title_short	Conjugated polymers mediate effective activation of the Mammalian Ion Channel Transient Receptor Potential Vanilloid 1
title_full	Conjugated polymers mediate effective activation of the Mammalian Ion Channel Transient Receptor Potential Vanilloid 1
title_fullStr	Conjugated polymers mediate effective activation of the Mammalian Ion Channel Transient Receptor Potential Vanilloid 1
title_full_unstemmed	Conjugated polymers mediate effective activation of the Mammalian Ion Channel Transient Receptor Potential Vanilloid 1
title_sort	conjugated polymers mediate effective activation of the mammalian ion channel transient receptor potential vanilloid 1
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ea056b5203764a46914a56ced95195e8
work_keys_str_mv	AT flodola conjugatedpolymersmediateeffectiveactivationofthemammalianionchanneltransientreceptorpotentialvanilloid1 AT nmartino conjugatedpolymersmediateeffectiveactivationofthemammalianionchanneltransientreceptorpotentialvanilloid1 AT gtullii conjugatedpolymersmediateeffectiveactivationofthemammalianionchanneltransientreceptorpotentialvanilloid1 AT glanzani conjugatedpolymersmediateeffectiveactivationofthemammalianionchanneltransientreceptorpotentialvanilloid1 AT mrantognazza conjugatedpolymersmediateeffectiveactivationofthemammalianionchanneltransientreceptorpotentialvanilloid1
_version_	1718395019292835840

Conjugated polymers mediate effective activation of the Mammalian Ion Channel Transient Receptor Potential Vanilloid 1

Ejemplares similares