Comparative genome analysis of Mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease.

Mycobacterium avium complex (MAC) infection causes disseminated disease in immunocompromised hosts, such as human immunodeficiency virus (HIV)-positive patients, and pulmonary disease in persons without systemic immunosuppression, which has been increasing in many countries. In Japan, the incidence...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kei-ichi Uchiya, Hiroyasu Takahashi, Tetsuya Yagi, Makoto Moriyama, Takayuki Inagaki, Kazuya Ichikawa, Taku Nakagawa, Toshiaki Nikai, Kenji Ogawa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ea15867fa5894df9be8b4c2ceef96abe
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ea15867fa5894df9be8b4c2ceef96abe
record_format	dspace
spelling	oai:doaj.org-article:ea15867fa5894df9be8b4c2ceef96abe2021-11-18T08:58:37ZComparative genome analysis of Mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease.1932-620310.1371/journal.pone.0071831https://doaj.org/article/ea15867fa5894df9be8b4c2ceef96abe2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23990995/?tool=EBIhttps://doaj.org/toc/1932-6203Mycobacterium avium complex (MAC) infection causes disseminated disease in immunocompromised hosts, such as human immunodeficiency virus (HIV)-positive patients, and pulmonary disease in persons without systemic immunosuppression, which has been increasing in many countries. In Japan, the incidence of pulmonary MAC disease caused by M. avium is about 7 times higher than that caused by M. intracellulare. To explore the bacterial factors that affect the pathological state of MAC disease caused by M. avium, we determined the complete genome sequence of the previously unreported M. avium subsp. hominissuis strain TH135 isolated from a HIV-negative patient with pulmonary MAC disease and compared it with the known genomic sequence of M. avium strain 104 derived from an acquired immunodeficiency syndrome patient with MAC disease. The genome of strain TH135 consists of a 4,951,217-bp circular chromosome with 4,636 coding sequences. Comparative analysis revealed that 4,012 genes are shared between the two strains, and strains TH135 and 104 have 624 and 1,108 unique genes, respectively. Many strain-specific regions including virulence-associated genes were found in genomes of both strains, and except for some regions, the G+C content in the specific regions was low compared with the mean G+C content of the corresponding chromosome. Screening of clinical isolates for genes located in the strain-specific regions revealed that the detection rates of strain TH135-specific genes were relatively high in specimens isolated from pulmonary MAC disease patients, while, those of strain 104-specific genes were relatively high in those from HIV-positive patients. Collectively, M. avium strains that cause pulmonary and disseminated disease possess genetically distinct features, and it suggests that the acquisition of specific genes during strain evolution has played an important role in the pathological manifestations of MAC disease.Kei-ichi UchiyaHiroyasu TakahashiTetsuya YagiMakoto MoriyamaTakayuki InagakiKazuya IchikawaTaku NakagawaToshiaki NikaiKenji OgawaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e71831 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kei-ichi Uchiya Hiroyasu Takahashi Tetsuya Yagi Makoto Moriyama Takayuki Inagaki Kazuya Ichikawa Taku Nakagawa Toshiaki Nikai Kenji Ogawa Comparative genome analysis of Mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease.
description	Mycobacterium avium complex (MAC) infection causes disseminated disease in immunocompromised hosts, such as human immunodeficiency virus (HIV)-positive patients, and pulmonary disease in persons without systemic immunosuppression, which has been increasing in many countries. In Japan, the incidence of pulmonary MAC disease caused by M. avium is about 7 times higher than that caused by M. intracellulare. To explore the bacterial factors that affect the pathological state of MAC disease caused by M. avium, we determined the complete genome sequence of the previously unreported M. avium subsp. hominissuis strain TH135 isolated from a HIV-negative patient with pulmonary MAC disease and compared it with the known genomic sequence of M. avium strain 104 derived from an acquired immunodeficiency syndrome patient with MAC disease. The genome of strain TH135 consists of a 4,951,217-bp circular chromosome with 4,636 coding sequences. Comparative analysis revealed that 4,012 genes are shared between the two strains, and strains TH135 and 104 have 624 and 1,108 unique genes, respectively. Many strain-specific regions including virulence-associated genes were found in genomes of both strains, and except for some regions, the G+C content in the specific regions was low compared with the mean G+C content of the corresponding chromosome. Screening of clinical isolates for genes located in the strain-specific regions revealed that the detection rates of strain TH135-specific genes were relatively high in specimens isolated from pulmonary MAC disease patients, while, those of strain 104-specific genes were relatively high in those from HIV-positive patients. Collectively, M. avium strains that cause pulmonary and disseminated disease possess genetically distinct features, and it suggests that the acquisition of specific genes during strain evolution has played an important role in the pathological manifestations of MAC disease.
format	article
author	Kei-ichi Uchiya Hiroyasu Takahashi Tetsuya Yagi Makoto Moriyama Takayuki Inagaki Kazuya Ichikawa Taku Nakagawa Toshiaki Nikai Kenji Ogawa
author_facet	Kei-ichi Uchiya Hiroyasu Takahashi Tetsuya Yagi Makoto Moriyama Takayuki Inagaki Kazuya Ichikawa Taku Nakagawa Toshiaki Nikai Kenji Ogawa
author_sort	Kei-ichi Uchiya
title	Comparative genome analysis of Mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease.
title_short	Comparative genome analysis of Mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease.
title_full	Comparative genome analysis of Mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease.
title_fullStr	Comparative genome analysis of Mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease.
title_full_unstemmed	Comparative genome analysis of Mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease.
title_sort	comparative genome analysis of mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ea15867fa5894df9be8b4c2ceef96abe
work_keys_str_mv	AT keiichiuchiya comparativegenomeanalysisofmycobacteriumaviumrevealedgeneticdiversityinstrainsthatcausepulmonaryanddisseminateddisease AT hiroyasutakahashi comparativegenomeanalysisofmycobacteriumaviumrevealedgeneticdiversityinstrainsthatcausepulmonaryanddisseminateddisease AT tetsuyayagi comparativegenomeanalysisofmycobacteriumaviumrevealedgeneticdiversityinstrainsthatcausepulmonaryanddisseminateddisease AT makotomoriyama comparativegenomeanalysisofmycobacteriumaviumrevealedgeneticdiversityinstrainsthatcausepulmonaryanddisseminateddisease AT takayukiinagaki comparativegenomeanalysisofmycobacteriumaviumrevealedgeneticdiversityinstrainsthatcausepulmonaryanddisseminateddisease AT kazuyaichikawa comparativegenomeanalysisofmycobacteriumaviumrevealedgeneticdiversityinstrainsthatcausepulmonaryanddisseminateddisease AT takunakagawa comparativegenomeanalysisofmycobacteriumaviumrevealedgeneticdiversityinstrainsthatcausepulmonaryanddisseminateddisease AT toshiakinikai comparativegenomeanalysisofmycobacteriumaviumrevealedgeneticdiversityinstrainsthatcausepulmonaryanddisseminateddisease AT kenjiogawa comparativegenomeanalysisofmycobacteriumaviumrevealedgeneticdiversityinstrainsthatcausepulmonaryanddisseminateddisease
_version_	1718421116367667200

Comparative genome analysis of Mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease.

Ejemplares similares