A split fluorescent reporter with rapid and reversible complementation

Monitoring protein-protein interactions via bimolecular fluorescence complementation is often limited by the slow kinetics and irreversibility of the complementation. Here the authors introduce a fluorescent reporter for real-time monitoring of reversible interactions based on complementation and bi...

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Detalles Bibliográficos
Autores principales:	Alison G. Tebo, Arnaud Gautier
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ea17b9f6917e45d5a4a74a03b83be033
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spelling	oai:doaj.org-article:ea17b9f6917e45d5a4a74a03b83be0332021-12-02T15:36:23ZA split fluorescent reporter with rapid and reversible complementation10.1038/s41467-019-10855-02041-1723https://doaj.org/article/ea17b9f6917e45d5a4a74a03b83be0332019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10855-0https://doaj.org/toc/2041-1723Monitoring protein-protein interactions via bimolecular fluorescence complementation is often limited by the slow kinetics and irreversibility of the complementation. Here the authors introduce a fluorescent reporter for real-time monitoring of reversible interactions based on complementation and binding of an exogenous chromophore.Alison G. TeboArnaud GautierNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Alison G. Tebo Arnaud Gautier A split fluorescent reporter with rapid and reversible complementation
description	Monitoring protein-protein interactions via bimolecular fluorescence complementation is often limited by the slow kinetics and irreversibility of the complementation. Here the authors introduce a fluorescent reporter for real-time monitoring of reversible interactions based on complementation and binding of an exogenous chromophore.
format	article
author	Alison G. Tebo Arnaud Gautier
author_facet	Alison G. Tebo Arnaud Gautier
author_sort	Alison G. Tebo
title	A split fluorescent reporter with rapid and reversible complementation
title_short	A split fluorescent reporter with rapid and reversible complementation
title_full	A split fluorescent reporter with rapid and reversible complementation
title_fullStr	A split fluorescent reporter with rapid and reversible complementation
title_full_unstemmed	A split fluorescent reporter with rapid and reversible complementation
title_sort	split fluorescent reporter with rapid and reversible complementation
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/ea17b9f6917e45d5a4a74a03b83be033
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A split fluorescent reporter with rapid and reversible complementation

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