Phage therapy in antibiotic resistant pneumonia: immunomodulation or redistribution?

Our report concerns the observations made during the treatment of pneumonia with individually selected bacteriophages in HCAI patients on mechanical ventilation. 19 patients on mechanical ventilation whose condition was complicated by antibiotic-resistant pneumonia were examined. The treatment of pa...

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Autores principales:	S. S. Bochkareva, I. M. Fedorova, O. N. Ershova, S. I. Koteleva, I. V. Kapustin, M. S. Blyakher, L. I. Novikova, A. V. Aleshkin, А. М. Vorobev
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	phage therapy effects on the immune system activated t lymphocytes nk cells ifnγ ifnα antibiotic-resistant pneumonia Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ea180e8e489042858b8bfec5531a3298
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spelling	oai:doaj.org-article:ea180e8e489042858b8bfec5531a32982021-11-18T08:03:50ZPhage therapy in antibiotic resistant pneumonia: immunomodulation or redistribution?1563-06252313-741X10.15789/1563-0625-PTI-2012https://doaj.org/article/ea180e8e489042858b8bfec5531a32982021-03-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2012https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XOur report concerns the observations made during the treatment of pneumonia with individually selected bacteriophages in HCAI patients on mechanical ventilation. 19 patients on mechanical ventilation whose condition was complicated by antibiotic-resistant pneumonia were examined. The treatment of patients was supplemented with phage therapy, bacteriophages were selected individually for each patient, taking into account the microbial etiology of the disease (Pseudomonas aeruginosa, Кlebsiella pneumoniae, Acinetobacter baumanii). Immunophenotyping of blood lymphocytes was carried out using 2-3-parameter flow cytometry. The functional activity of blood leukocytes was assessed by their ability to produce IFNα and IFNγ during cultivation. The level of interferons production in supernatants collected after cultivation was quantitatively evaluated both by their concentration (ELISA, reagents from “Vector-Best-Europe”, Russia) and by their biological activity. Statistical processing of the results was carried out using the Statistica 6 program according to the nonparametric Mann-Whitney U-test. In the course of successful phage therapy with individually selected bacteriophages overcoming of lymphopenia (if there was one) and an increase in both the number and functional activity of peripheral blood lymphocytes in all patients with pneumonia observed are noted. The relationship between the microbial load (mono- or mixed infection, the number of CFU pathogens of pneumonia, the need for repeated courses of phage therapy) and the degree of deficiency in one or another subpopulation of lymphocytes was not detected. Activation of the immune system achieved after one course of phage therapy was maintained for at least 3 weeks after phage administration was discontinued.S. S. BochkarevaI. M. FedorovaO. N. ErshovaS. I. KotelevaI. V. KapustinM. S. BlyakherL. I. NovikovaA. V. AleshkinА. М. VorobevSPb RAACIarticlephage therapyeffects on the immune systemactivated t lymphocytesnk cellsifnγifnαantibiotic-resistant pneumoniaImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 1, Pp 179-184 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	phage therapy effects on the immune system activated t lymphocytes nk cells ifnγ ifnα antibiotic-resistant pneumonia Immunologic diseases. Allergy RC581-607
spellingShingle	phage therapy effects on the immune system activated t lymphocytes nk cells ifnγ ifnα antibiotic-resistant pneumonia Immunologic diseases. Allergy RC581-607 S. S. Bochkareva I. M. Fedorova O. N. Ershova S. I. Koteleva I. V. Kapustin M. S. Blyakher L. I. Novikova A. V. Aleshkin А. М. Vorobev Phage therapy in antibiotic resistant pneumonia: immunomodulation or redistribution?
description	Our report concerns the observations made during the treatment of pneumonia with individually selected bacteriophages in HCAI patients on mechanical ventilation. 19 patients on mechanical ventilation whose condition was complicated by antibiotic-resistant pneumonia were examined. The treatment of patients was supplemented with phage therapy, bacteriophages were selected individually for each patient, taking into account the microbial etiology of the disease (Pseudomonas aeruginosa, Кlebsiella pneumoniae, Acinetobacter baumanii). Immunophenotyping of blood lymphocytes was carried out using 2-3-parameter flow cytometry. The functional activity of blood leukocytes was assessed by their ability to produce IFNα and IFNγ during cultivation. The level of interferons production in supernatants collected after cultivation was quantitatively evaluated both by their concentration (ELISA, reagents from “Vector-Best-Europe”, Russia) and by their biological activity. Statistical processing of the results was carried out using the Statistica 6 program according to the nonparametric Mann-Whitney U-test. In the course of successful phage therapy with individually selected bacteriophages overcoming of lymphopenia (if there was one) and an increase in both the number and functional activity of peripheral blood lymphocytes in all patients with pneumonia observed are noted. The relationship between the microbial load (mono- or mixed infection, the number of CFU pathogens of pneumonia, the need for repeated courses of phage therapy) and the degree of deficiency in one or another subpopulation of lymphocytes was not detected. Activation of the immune system achieved after one course of phage therapy was maintained for at least 3 weeks after phage administration was discontinued.
format	article
author	S. S. Bochkareva I. M. Fedorova O. N. Ershova S. I. Koteleva I. V. Kapustin M. S. Blyakher L. I. Novikova A. V. Aleshkin А. М. Vorobev
author_facet	S. S. Bochkareva I. M. Fedorova O. N. Ershova S. I. Koteleva I. V. Kapustin M. S. Blyakher L. I. Novikova A. V. Aleshkin А. М. Vorobev
author_sort	S. S. Bochkareva
title	Phage therapy in antibiotic resistant pneumonia: immunomodulation or redistribution?
title_short	Phage therapy in antibiotic resistant pneumonia: immunomodulation or redistribution?
title_full	Phage therapy in antibiotic resistant pneumonia: immunomodulation or redistribution?
title_fullStr	Phage therapy in antibiotic resistant pneumonia: immunomodulation or redistribution?
title_full_unstemmed	Phage therapy in antibiotic resistant pneumonia: immunomodulation or redistribution?
title_sort	phage therapy in antibiotic resistant pneumonia: immunomodulation or redistribution?
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/ea180e8e489042858b8bfec5531a3298
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Phage therapy in antibiotic resistant pneumonia: immunomodulation or redistribution?

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