Relationships between cell cycle regulator gene copy numbers and protein expression levels in Schizosaccharomyces pombe.

We previously determined the copy number limits of overexpression for cell division cycle (cdc) regulatory genes in the fission yeast Schizosaccharomyces pombe using the "genetic tug-of-war" (gTOW) method. In this study, we measured the levels of tandem affinity purification (TAP)-tagged t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ayako Chino, Koji Makanae, Hisao Moriya
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ea197515343e4fd5a539f7f04c0cecc7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ea197515343e4fd5a539f7f04c0cecc7
record_format	dspace
spelling	oai:doaj.org-article:ea197515343e4fd5a539f7f04c0cecc72021-11-18T08:57:12ZRelationships between cell cycle regulator gene copy numbers and protein expression levels in Schizosaccharomyces pombe.1932-620310.1371/journal.pone.0073319https://doaj.org/article/ea197515343e4fd5a539f7f04c0cecc72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24019917/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We previously determined the copy number limits of overexpression for cell division cycle (cdc) regulatory genes in the fission yeast Schizosaccharomyces pombe using the "genetic tug-of-war" (gTOW) method. In this study, we measured the levels of tandem affinity purification (TAP)-tagged target proteins when their copy numbers are increased in gTOW. Twenty analyzed genes showed roughly linear correlations between increased protein levels and gene copy numbers, which suggested a general lack of compensation for gene dosage in S. pombe. Cdc16 and Sid2 protein levels but not their mRNA levels were much lower than that expected by their copy numbers, which suggested the existence of a post-transcriptional down regulation of these genes. The cyclin Cig1 protein level and its mRNA level were much higher than that expected by its copy numbers, which suggested a positive feedback mechanism for its expression. A higher Cdc10 protein level and its mRNA level, probably due to cloning its gene into a plasmid, indicated that Cdc10 regulation was more robust than that previously predicted.Ayako ChinoKoji MakanaeHisao MoriyaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e73319 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ayako Chino Koji Makanae Hisao Moriya Relationships between cell cycle regulator gene copy numbers and protein expression levels in Schizosaccharomyces pombe.
description	We previously determined the copy number limits of overexpression for cell division cycle (cdc) regulatory genes in the fission yeast Schizosaccharomyces pombe using the "genetic tug-of-war" (gTOW) method. In this study, we measured the levels of tandem affinity purification (TAP)-tagged target proteins when their copy numbers are increased in gTOW. Twenty analyzed genes showed roughly linear correlations between increased protein levels and gene copy numbers, which suggested a general lack of compensation for gene dosage in S. pombe. Cdc16 and Sid2 protein levels but not their mRNA levels were much lower than that expected by their copy numbers, which suggested the existence of a post-transcriptional down regulation of these genes. The cyclin Cig1 protein level and its mRNA level were much higher than that expected by its copy numbers, which suggested a positive feedback mechanism for its expression. A higher Cdc10 protein level and its mRNA level, probably due to cloning its gene into a plasmid, indicated that Cdc10 regulation was more robust than that previously predicted.
format	article
author	Ayako Chino Koji Makanae Hisao Moriya
author_facet	Ayako Chino Koji Makanae Hisao Moriya
author_sort	Ayako Chino
title	Relationships between cell cycle regulator gene copy numbers and protein expression levels in Schizosaccharomyces pombe.
title_short	Relationships between cell cycle regulator gene copy numbers and protein expression levels in Schizosaccharomyces pombe.
title_full	Relationships between cell cycle regulator gene copy numbers and protein expression levels in Schizosaccharomyces pombe.
title_fullStr	Relationships between cell cycle regulator gene copy numbers and protein expression levels in Schizosaccharomyces pombe.
title_full_unstemmed	Relationships between cell cycle regulator gene copy numbers and protein expression levels in Schizosaccharomyces pombe.
title_sort	relationships between cell cycle regulator gene copy numbers and protein expression levels in schizosaccharomyces pombe.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ea197515343e4fd5a539f7f04c0cecc7
work_keys_str_mv	AT ayakochino relationshipsbetweencellcycleregulatorgenecopynumbersandproteinexpressionlevelsinschizosaccharomycespombe AT kojimakanae relationshipsbetweencellcycleregulatorgenecopynumbersandproteinexpressionlevelsinschizosaccharomycespombe AT hisaomoriya relationshipsbetweencellcycleregulatorgenecopynumbersandproteinexpressionlevelsinschizosaccharomycespombe
_version_	1718421116579479552

Relationships between cell cycle regulator gene copy numbers and protein expression levels in Schizosaccharomyces pombe.

Ejemplares similares