Mutations in Transcriptional Regulators Allow Selective Engineering of Signal Integration Logic

ABSTRACT Bacterial cells monitor their environment by sensing a set of signals. Typically, these environmental signals affect promoter activities by altering the activity of transcription regulatory proteins. Promoters are often regulated by more than one regulatory protein, and in these cases the r...

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Autor principal:	Szabolcs Semsey
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ea1f7d3503da4acba21f1ab3961614a3
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spelling	oai:doaj.org-article:ea1f7d3503da4acba21f1ab3961614a32021-11-15T15:47:38ZMutations in Transcriptional Regulators Allow Selective Engineering of Signal Integration Logic10.1128/mBio.01171-142150-7511https://doaj.org/article/ea1f7d3503da4acba21f1ab3961614a32014-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01171-14https://doaj.org/toc/2150-7511ABSTRACT Bacterial cells monitor their environment by sensing a set of signals. Typically, these environmental signals affect promoter activities by altering the activity of transcription regulatory proteins. Promoters are often regulated by more than one regulatory protein, and in these cases the relevant signals are integrated by certain logic. In this work, we study how single amino acid substitutions in a regulatory protein (GalR) affect transcriptional regulation and signal integration logic at a set of engineered promoters. Our results suggest that point mutations in regulatory genes allow independent evolution of regulatory logic at different promoters. IMPORTANCE Gene regulatory networks are built from simple building blocks, such as promoters, transcription regulatory proteins, and their binding sites on DNA. Many promoters are regulated by more than one regulatory input. In these cases, the inputs are integrated and allow transcription only in certain combinations of input signals. Gene regulatory networks can be easily rewired, because the function of cis-regulatory elements and promoters can be altered by point mutations. In this work, we tested how point mutations in transcription regulatory proteins can affect signal integration logic. We found that such mutations allow context-dependent engineering of signal integration logic at promoters, further contributing to the plasticity of gene regulatory networks.Szabolcs SemseyAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 3 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Szabolcs Semsey Mutations in Transcriptional Regulators Allow Selective Engineering of Signal Integration Logic
description	ABSTRACT Bacterial cells monitor their environment by sensing a set of signals. Typically, these environmental signals affect promoter activities by altering the activity of transcription regulatory proteins. Promoters are often regulated by more than one regulatory protein, and in these cases the relevant signals are integrated by certain logic. In this work, we study how single amino acid substitutions in a regulatory protein (GalR) affect transcriptional regulation and signal integration logic at a set of engineered promoters. Our results suggest that point mutations in regulatory genes allow independent evolution of regulatory logic at different promoters. IMPORTANCE Gene regulatory networks are built from simple building blocks, such as promoters, transcription regulatory proteins, and their binding sites on DNA. Many promoters are regulated by more than one regulatory input. In these cases, the inputs are integrated and allow transcription only in certain combinations of input signals. Gene regulatory networks can be easily rewired, because the function of cis-regulatory elements and promoters can be altered by point mutations. In this work, we tested how point mutations in transcription regulatory proteins can affect signal integration logic. We found that such mutations allow context-dependent engineering of signal integration logic at promoters, further contributing to the plasticity of gene regulatory networks.
format	article
author	Szabolcs Semsey
author_facet	Szabolcs Semsey
author_sort	Szabolcs Semsey
title	Mutations in Transcriptional Regulators Allow Selective Engineering of Signal Integration Logic
title_short	Mutations in Transcriptional Regulators Allow Selective Engineering of Signal Integration Logic
title_full	Mutations in Transcriptional Regulators Allow Selective Engineering of Signal Integration Logic
title_fullStr	Mutations in Transcriptional Regulators Allow Selective Engineering of Signal Integration Logic
title_full_unstemmed	Mutations in Transcriptional Regulators Allow Selective Engineering of Signal Integration Logic
title_sort	mutations in transcriptional regulators allow selective engineering of signal integration logic
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/ea1f7d3503da4acba21f1ab3961614a3
work_keys_str_mv	AT szabolcssemsey mutationsintranscriptionalregulatorsallowselectiveengineeringofsignalintegrationlogic
_version_	1718427555078340608

Mutations in Transcriptional Regulators Allow Selective Engineering of Signal Integration Logic

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