Global Mapping of the Macrophage-HIV-1 Transcriptome Reveals that Productive Infection Induces Remodeling of Host Cell DNA and Chromatin

Abstract It has been proposed that macrophages could serve as long-lived compartments for HIV-1 infection under in vivo situations because these cells are resistant to the virus-mediated cytopathic effect, produce progeny virus over extended periods of time and are localized in tissues that are ofte...

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Autores principales:	Alexandre Deshiere, Charles Joly-Beauparlant, Yann Breton, Michel Ouellet, Frédéric Raymond, Robert Lodge, Corinne Barat, Marc-André Roy, Jacques Corbeil, Michel J. Tremblay
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ea2329ad91314c6c8c74950e07bd5937
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spelling	oai:doaj.org-article:ea2329ad91314c6c8c74950e07bd59372021-12-02T15:05:11ZGlobal Mapping of the Macrophage-HIV-1 Transcriptome Reveals that Productive Infection Induces Remodeling of Host Cell DNA and Chromatin10.1038/s41598-017-05566-92045-2322https://doaj.org/article/ea2329ad91314c6c8c74950e07bd59372017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05566-9https://doaj.org/toc/2045-2322Abstract It has been proposed that macrophages could serve as long-lived compartments for HIV-1 infection under in vivo situations because these cells are resistant to the virus-mediated cytopathic effect, produce progeny virus over extended periods of time and are localized in tissues that are often less accessible by treatment. Comprehensive experimental studies are thus needed to characterize the HIV-1-induced modulation of host genes in these myeloid lineage cells. To shed light on this important issue, we performed comparative analyses of mRNA expression levels of host genes in uninfected bystander and HIV-1-infected human macrophages using an infectious reporter virus construct coupled with a large-scale RNA sequencing approach. We observed a rapid differential expression of several host factors in the productively infected macrophage population including genes regulating DNA replication factors and chromatin remodeling. A siRNA-mediated screening study to functionally identify host determinants involved in HIV-1 biology has provided new information on the virus molecular regulation in macrophages.Alexandre DeshiereCharles Joly-BeauparlantYann BretonMichel OuelletFrédéric RaymondRobert LodgeCorinne BaratMarc-André RoyJacques CorbeilMichel J. TremblayNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexandre Deshiere Charles Joly-Beauparlant Yann Breton Michel Ouellet Frédéric Raymond Robert Lodge Corinne Barat Marc-André Roy Jacques Corbeil Michel J. Tremblay Global Mapping of the Macrophage-HIV-1 Transcriptome Reveals that Productive Infection Induces Remodeling of Host Cell DNA and Chromatin
description	Abstract It has been proposed that macrophages could serve as long-lived compartments for HIV-1 infection under in vivo situations because these cells are resistant to the virus-mediated cytopathic effect, produce progeny virus over extended periods of time and are localized in tissues that are often less accessible by treatment. Comprehensive experimental studies are thus needed to characterize the HIV-1-induced modulation of host genes in these myeloid lineage cells. To shed light on this important issue, we performed comparative analyses of mRNA expression levels of host genes in uninfected bystander and HIV-1-infected human macrophages using an infectious reporter virus construct coupled with a large-scale RNA sequencing approach. We observed a rapid differential expression of several host factors in the productively infected macrophage population including genes regulating DNA replication factors and chromatin remodeling. A siRNA-mediated screening study to functionally identify host determinants involved in HIV-1 biology has provided new information on the virus molecular regulation in macrophages.
format	article
author	Alexandre Deshiere Charles Joly-Beauparlant Yann Breton Michel Ouellet Frédéric Raymond Robert Lodge Corinne Barat Marc-André Roy Jacques Corbeil Michel J. Tremblay
author_facet	Alexandre Deshiere Charles Joly-Beauparlant Yann Breton Michel Ouellet Frédéric Raymond Robert Lodge Corinne Barat Marc-André Roy Jacques Corbeil Michel J. Tremblay
author_sort	Alexandre Deshiere
title	Global Mapping of the Macrophage-HIV-1 Transcriptome Reveals that Productive Infection Induces Remodeling of Host Cell DNA and Chromatin
title_short	Global Mapping of the Macrophage-HIV-1 Transcriptome Reveals that Productive Infection Induces Remodeling of Host Cell DNA and Chromatin
title_full	Global Mapping of the Macrophage-HIV-1 Transcriptome Reveals that Productive Infection Induces Remodeling of Host Cell DNA and Chromatin
title_fullStr	Global Mapping of the Macrophage-HIV-1 Transcriptome Reveals that Productive Infection Induces Remodeling of Host Cell DNA and Chromatin
title_full_unstemmed	Global Mapping of the Macrophage-HIV-1 Transcriptome Reveals that Productive Infection Induces Remodeling of Host Cell DNA and Chromatin
title_sort	global mapping of the macrophage-hiv-1 transcriptome reveals that productive infection induces remodeling of host cell dna and chromatin
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ea2329ad91314c6c8c74950e07bd5937
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Global Mapping of the Macrophage-HIV-1 Transcriptome Reveals that Productive Infection Induces Remodeling of Host Cell DNA and Chromatin

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