BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo

Human embryonic stem cells (hESCs) possess an immense potential to generate clinically relevant cell types and unveil mechanisms underlying early human development. However, using hESCs for discovery or translation requires accurately identifying differentiated cell types through comparison with the...

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Autores principales:	Sapna Chhabra, Aryeh Warmflash
Formato:	article
Lenguaje:	EN
Publicado:	The Company of Biologists 2021
Materias:	amnion differentiation human embryonic stem cells scrna-seq trophectoderm bmp4 extra-embryonic mesoder Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ea2d9a68b850493db62054d63e60eaaf
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spelling	oai:doaj.org-article:ea2d9a68b850493db62054d63e60eaaf2021-11-28T16:01:08ZBMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo2046-639010.1242/bio.058617https://doaj.org/article/ea2d9a68b850493db62054d63e60eaaf2021-09-01T00:00:00Zhttp://bio.biologists.org/content/10/9/bio058617https://doaj.org/toc/2046-6390Human embryonic stem cells (hESCs) possess an immense potential to generate clinically relevant cell types and unveil mechanisms underlying early human development. However, using hESCs for discovery or translation requires accurately identifying differentiated cell types through comparison with their in vivo counterparts. Here, we set out to determine the identity of much debated BMP-treated hESCs by comparing their transcriptome to recently published single cell transcriptomic data from early human embryos ( Xiang et al., 2020). Our analyses reveal several discrepancies in the published human embryo dataset, including misclassification of putative amnion, intermediate and inner cell mass cells. These misclassifications primarily resulted from similarities in pseudogene expression, highlighting the need to carefully consider gene lists when making comparisons between cell types. In the absence of a relevant human dataset, we utilized the recently published single cell transcriptome of the early post implantation monkey embryo to discern the identity of BMP-treated hESCs. Our results suggest that BMP-treated hESCs are transcriptionally more similar to amnion cells than trophectoderm cells in the monkey embryo. Together with prior studies, this result indicates that hESCs possess a unique ability to form mature trophectoderm subtypes via an amnion-like transcriptional state. This article has an associated First Person interview with the first author of the paper.Sapna ChhabraAryeh WarmflashThe Company of Biologistsarticleamniondifferentiationhuman embryonic stem cellsscrna-seqtrophectodermbmp4extra-embryonic mesoderScienceQBiology (General)QH301-705.5ENBiology Open, Vol 10, Iss 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	amnion differentiation human embryonic stem cells scrna-seq trophectoderm bmp4 extra-embryonic mesoder Science Q Biology (General) QH301-705.5
spellingShingle	amnion differentiation human embryonic stem cells scrna-seq trophectoderm bmp4 extra-embryonic mesoder Science Q Biology (General) QH301-705.5 Sapna Chhabra Aryeh Warmflash BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo
description	Human embryonic stem cells (hESCs) possess an immense potential to generate clinically relevant cell types and unveil mechanisms underlying early human development. However, using hESCs for discovery or translation requires accurately identifying differentiated cell types through comparison with their in vivo counterparts. Here, we set out to determine the identity of much debated BMP-treated hESCs by comparing their transcriptome to recently published single cell transcriptomic data from early human embryos ( Xiang et al., 2020). Our analyses reveal several discrepancies in the published human embryo dataset, including misclassification of putative amnion, intermediate and inner cell mass cells. These misclassifications primarily resulted from similarities in pseudogene expression, highlighting the need to carefully consider gene lists when making comparisons between cell types. In the absence of a relevant human dataset, we utilized the recently published single cell transcriptome of the early post implantation monkey embryo to discern the identity of BMP-treated hESCs. Our results suggest that BMP-treated hESCs are transcriptionally more similar to amnion cells than trophectoderm cells in the monkey embryo. Together with prior studies, this result indicates that hESCs possess a unique ability to form mature trophectoderm subtypes via an amnion-like transcriptional state. This article has an associated First Person interview with the first author of the paper.
format	article
author	Sapna Chhabra Aryeh Warmflash
author_facet	Sapna Chhabra Aryeh Warmflash
author_sort	Sapna Chhabra
title	BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo
title_short	BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo
title_full	BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo
title_fullStr	BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo
title_full_unstemmed	BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo
title_sort	bmp-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo
publisher	The Company of Biologists
publishDate	2021
url	https://doaj.org/article/ea2d9a68b850493db62054d63e60eaaf
work_keys_str_mv	AT sapnachhabra bmptreatedhumanembryonicstemcellstranscriptionallyresembleamnioncellsinthemonkeyembryo AT aryehwarmflash bmptreatedhumanembryonicstemcellstranscriptionallyresembleamnioncellsinthemonkeyembryo
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BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo

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