Genetic Characterization of Corynebacterium pseudotuberculosis Isolates in Egypt

Corynebacterium pseudotuberculosis is a small Gram-positive bacillus containing mycolic acid in the structure of the cell wall. The bacterium is responsible for Caseous Lymphadenitis (CLA) in small ruminants (sheep and goats). The bacteria are also responsible for Ulcerative Lymphangitis in equines....

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Autores principales:	Nadine A. El-Sebay, Marwah M. Mohamed, Elham F. El-Sergany, Ashraf M. Abbas, Roukaya M. Osman, Dalia A. M. Abd El-Moaty
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	c. pseudotuberculosis pld gene quadruplex pcr sequence analysis Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2021.154576 https://doaj.org/article/ea2fa004fe8a40579862a7549676ad3f
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spelling	oai:doaj.org-article:ea2fa004fe8a40579862a7549676ad3f2021-12-02T18:03:08ZGenetic Characterization of Corynebacterium pseudotuberculosis Isolates in Egypthttps://dx.doi.org/10.21608/javs.2021.1545761687-40722090-3308https://doaj.org/article/ea2fa004fe8a40579862a7549676ad3f2021-04-01T00:00:00Zhttps://javs.journals.ekb.eg/article_154576.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Corynebacterium pseudotuberculosis is a small Gram-positive bacillus containing mycolic acid in the structure of the cell wall. The bacterium is responsible for Caseous Lymphadenitis (CLA) in small ruminants (sheep and goats). The bacteria are also responsible for Ulcerative Lymphangitis in equines. The disease causes great economic loss in the animal industry. This work aimed to check the ability of Quadruplex PCR (Q-PCR) for genotyping and identification of Egyptian isolates of C. pseudotuberculosis and sequence analysis of phospholipase D (PLD) gene of local isolates. Four of C. pseudotuberculosis local isolates previously biochemically identified were tested for narG gene (nitrate reductase gene). Both nitrate negative biovar (ovis) and nitrate positive biovar (equi) showed a positive result for 16S rRNA, rpoB and PLD genes of C. pseudotuberculosis species. The sequence analysis of our local isolates' PLD gene revealed minor changes in PLD proteins between ovine and equine strains compared with other published PLD sequences in GenBank. It was concluded that the Q-PCR method is able to differentiate between C. pseudotuberculosis equi and ovis biovars. Also, the sequence of PLD gene of local isolates representing the two biovars revealed some variation, which leads to an accurate diagnosis of C. pseudotuberculosis biovars and generates a mapping of immerged local isolates. Further, the PCR and sequence of these isolates provide rapid and accurate genotyping, especially with hyperimmune serum's unavailability. Nadine A. El-SebayMarwah M. MohamedElham F. El-SerganyAshraf M. AbbasRoukaya M. OsmanDalia A. M. Abd El-MoatyEgyptian Society for Animal Managementarticlec. pseudotuberculosispld genequadruplex pcrsequence analysisZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 2, Pp 15-21 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	c. pseudotuberculosis pld gene quadruplex pcr sequence analysis Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	c. pseudotuberculosis pld gene quadruplex pcr sequence analysis Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Nadine A. El-Sebay Marwah M. Mohamed Elham F. El-Sergany Ashraf M. Abbas Roukaya M. Osman Dalia A. M. Abd El-Moaty Genetic Characterization of Corynebacterium pseudotuberculosis Isolates in Egypt
description	Corynebacterium pseudotuberculosis is a small Gram-positive bacillus containing mycolic acid in the structure of the cell wall. The bacterium is responsible for Caseous Lymphadenitis (CLA) in small ruminants (sheep and goats). The bacteria are also responsible for Ulcerative Lymphangitis in equines. The disease causes great economic loss in the animal industry. This work aimed to check the ability of Quadruplex PCR (Q-PCR) for genotyping and identification of Egyptian isolates of C. pseudotuberculosis and sequence analysis of phospholipase D (PLD) gene of local isolates. Four of C. pseudotuberculosis local isolates previously biochemically identified were tested for narG gene (nitrate reductase gene). Both nitrate negative biovar (ovis) and nitrate positive biovar (equi) showed a positive result for 16S rRNA, rpoB and PLD genes of C. pseudotuberculosis species. The sequence analysis of our local isolates' PLD gene revealed minor changes in PLD proteins between ovine and equine strains compared with other published PLD sequences in GenBank. It was concluded that the Q-PCR method is able to differentiate between C. pseudotuberculosis equi and ovis biovars. Also, the sequence of PLD gene of local isolates representing the two biovars revealed some variation, which leads to an accurate diagnosis of C. pseudotuberculosis biovars and generates a mapping of immerged local isolates. Further, the PCR and sequence of these isolates provide rapid and accurate genotyping, especially with hyperimmune serum's unavailability.
format	article
author	Nadine A. El-Sebay Marwah M. Mohamed Elham F. El-Sergany Ashraf M. Abbas Roukaya M. Osman Dalia A. M. Abd El-Moaty
author_facet	Nadine A. El-Sebay Marwah M. Mohamed Elham F. El-Sergany Ashraf M. Abbas Roukaya M. Osman Dalia A. M. Abd El-Moaty
author_sort	Nadine A. El-Sebay
title	Genetic Characterization of Corynebacterium pseudotuberculosis Isolates in Egypt
title_short	Genetic Characterization of Corynebacterium pseudotuberculosis Isolates in Egypt
title_full	Genetic Characterization of Corynebacterium pseudotuberculosis Isolates in Egypt
title_fullStr	Genetic Characterization of Corynebacterium pseudotuberculosis Isolates in Egypt
title_full_unstemmed	Genetic Characterization of Corynebacterium pseudotuberculosis Isolates in Egypt
title_sort	genetic characterization of corynebacterium pseudotuberculosis isolates in egypt
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://dx.doi.org/10.21608/javs.2021.154576 https://doaj.org/article/ea2fa004fe8a40579862a7549676ad3f
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Genetic Characterization of Corynebacterium pseudotuberculosis Isolates in Egypt

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