Does each bead count? A reduced-cost approach for recovering waterborne protozoa from challenge water using immunomagnetic separation

Giardia duodenalis and Cryptosporidium spp. are two of the most prominent aetiological agents of waterborne diseases. Therefore, efficient and affordable methodologies for identifying and quantifying these parasites in water are increasingly necessary. USEPA Method 1623.1 is a widely used and valida...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Natália de Melo Nasser Fava, Kamila Jessie Sammarro Silva, William John Snelling, Nigel George Ternan, James Stephen Gerard Dooley, Lyda Patricia Sabogal-Paz
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	cryptosporidium spp. oocysts giardia spp. cysts low-cost recovery methods parasitic protozoa recovery efficiency Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/ea37d9424b484244b7b1c244a93a4f1c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ea37d9424b484244b7b1c244a93a4f1c
record_format	dspace
spelling	oai:doaj.org-article:ea37d9424b484244b7b1c244a93a4f1c2021-11-06T06:05:06ZDoes each bead count? A reduced-cost approach for recovering waterborne protozoa from challenge water using immunomagnetic separation1477-89201996-782910.2166/wh.2021.005https://doaj.org/article/ea37d9424b484244b7b1c244a93a4f1c2021-06-01T00:00:00Zhttp://jwh.iwaponline.com/content/19/3/436https://doaj.org/toc/1477-8920https://doaj.org/toc/1996-7829Giardia duodenalis and Cryptosporidium spp. are two of the most prominent aetiological agents of waterborne diseases. Therefore, efficient and affordable methodologies for identifying and quantifying these parasites in water are increasingly necessary. USEPA Method 1623.1 is a widely used and validated protocol for detecting these parasites in water samples. It consists of a concentration step, followed by parasite purification and visualization by immunofluorescence microscopy. Although efficient, this method has a high cost particularly due to the immunomagnetic separation (IMS) step, which is most needed with complex and highly contaminated samples. Based on this, the present study aimed to determine whether it is possible to maintain the efficiency of Method 1623.1 while reducing the amount of beads per reaction, using as a matrix the challenge water recommended by the World Health Organization. As for Giardia cysts, a satisfactory recovery efficiency (RE) was obtained using 50% less IMS beads. This was evaluated both with a commercial cyst suspension (56.1% recovery) and an analytical quality assessment (47.5% recovery). Although RE rates obtained for Cryptosporidium parvum did not meet Method 1623.1 criteria in any of the experimental conditions tested, results presented in this paper indicated the relevance of the described adaptations, even in challenge water. HIGHLIGHTS The high cost of current protozoa detection methods limits their widespread use in limited settings.; Immunomagnetic separation improves detection by cleaning the sample.; Recovery efficiency is maintained for Giardia duodenalis with 50% less beads.; Organisms adhering to beads after dissociation may impact recovery levels.;Natália de Melo Nasser FavaKamila Jessie Sammarro SilvaWilliam John SnellingNigel George TernanJames Stephen Gerard DooleyLyda Patricia Sabogal-PazIWA Publishingarticlecryptosporidium spp. oocystsgiardia spp. cystslow-cost recovery methodsparasitic protozoarecovery efficiencyPublic aspects of medicineRA1-1270ENJournal of Water and Health, Vol 19, Iss 3, Pp 436-447 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cryptosporidium spp. oocysts giardia spp. cysts low-cost recovery methods parasitic protozoa recovery efficiency Public aspects of medicine RA1-1270
spellingShingle	cryptosporidium spp. oocysts giardia spp. cysts low-cost recovery methods parasitic protozoa recovery efficiency Public aspects of medicine RA1-1270 Natália de Melo Nasser Fava Kamila Jessie Sammarro Silva William John Snelling Nigel George Ternan James Stephen Gerard Dooley Lyda Patricia Sabogal-Paz Does each bead count? A reduced-cost approach for recovering waterborne protozoa from challenge water using immunomagnetic separation
description	Giardia duodenalis and Cryptosporidium spp. are two of the most prominent aetiological agents of waterborne diseases. Therefore, efficient and affordable methodologies for identifying and quantifying these parasites in water are increasingly necessary. USEPA Method 1623.1 is a widely used and validated protocol for detecting these parasites in water samples. It consists of a concentration step, followed by parasite purification and visualization by immunofluorescence microscopy. Although efficient, this method has a high cost particularly due to the immunomagnetic separation (IMS) step, which is most needed with complex and highly contaminated samples. Based on this, the present study aimed to determine whether it is possible to maintain the efficiency of Method 1623.1 while reducing the amount of beads per reaction, using as a matrix the challenge water recommended by the World Health Organization. As for Giardia cysts, a satisfactory recovery efficiency (RE) was obtained using 50% less IMS beads. This was evaluated both with a commercial cyst suspension (56.1% recovery) and an analytical quality assessment (47.5% recovery). Although RE rates obtained for Cryptosporidium parvum did not meet Method 1623.1 criteria in any of the experimental conditions tested, results presented in this paper indicated the relevance of the described adaptations, even in challenge water. HIGHLIGHTS The high cost of current protozoa detection methods limits their widespread use in limited settings.; Immunomagnetic separation improves detection by cleaning the sample.; Recovery efficiency is maintained for Giardia duodenalis with 50% less beads.; Organisms adhering to beads after dissociation may impact recovery levels.;
format	article
author	Natália de Melo Nasser Fava Kamila Jessie Sammarro Silva William John Snelling Nigel George Ternan James Stephen Gerard Dooley Lyda Patricia Sabogal-Paz
author_facet	Natália de Melo Nasser Fava Kamila Jessie Sammarro Silva William John Snelling Nigel George Ternan James Stephen Gerard Dooley Lyda Patricia Sabogal-Paz
author_sort	Natália de Melo Nasser Fava
title	Does each bead count? A reduced-cost approach for recovering waterborne protozoa from challenge water using immunomagnetic separation
title_short	Does each bead count? A reduced-cost approach for recovering waterborne protozoa from challenge water using immunomagnetic separation
title_full	Does each bead count? A reduced-cost approach for recovering waterborne protozoa from challenge water using immunomagnetic separation
title_fullStr	Does each bead count? A reduced-cost approach for recovering waterborne protozoa from challenge water using immunomagnetic separation
title_full_unstemmed	Does each bead count? A reduced-cost approach for recovering waterborne protozoa from challenge water using immunomagnetic separation
title_sort	does each bead count? a reduced-cost approach for recovering waterborne protozoa from challenge water using immunomagnetic separation
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/ea37d9424b484244b7b1c244a93a4f1c
work_keys_str_mv	AT nataliademelonasserfava doeseachbeadcountareducedcostapproachforrecoveringwaterborneprotozoafromchallengewaterusingimmunomagneticseparation AT kamilajessiesammarrosilva doeseachbeadcountareducedcostapproachforrecoveringwaterborneprotozoafromchallengewaterusingimmunomagneticseparation AT williamjohnsnelling doeseachbeadcountareducedcostapproachforrecoveringwaterborneprotozoafromchallengewaterusingimmunomagneticseparation AT nigelgeorgeternan doeseachbeadcountareducedcostapproachforrecoveringwaterborneprotozoafromchallengewaterusingimmunomagneticseparation AT jamesstephengerarddooley doeseachbeadcountareducedcostapproachforrecoveringwaterborneprotozoafromchallengewaterusingimmunomagneticseparation AT lydapatriciasabogalpaz doeseachbeadcountareducedcostapproachforrecoveringwaterborneprotozoafromchallengewaterusingimmunomagneticseparation
_version_	1718443890342625280

Does each bead count? A reduced-cost approach for recovering waterborne protozoa from challenge water using immunomagnetic separation

Ejemplares similares