The effect of primer choice and short read sequences on the outcome of 16S rRNA gene based diversity studies.

Different regions of the bacterial 16S rRNA gene evolve at different evolutionary rates. The scientific outcome of short read sequencing studies therefore alters with the gene region sequenced. We wanted to gain insight in the impact of primer choice on the outcome of short read sequencing efforts....

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Bibliographic Details
Main Authors: Jonas Ghyselinck, Stefan Pfeiffer, Kim Heylen, Angela Sessitsch, Paul De Vos
Format: article
Language:EN
Published: Public Library of Science (PLoS) 2013
Subjects:
R
Q
Online Access:https://doaj.org/article/ea3b8b9b92254950b771d94543b9bc2a
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