MicroRNA miR-874-3p inhibits osteoporosis by targeting leptin (LEP)

MicroRNAs (miRNAs) regulate osteogenic differentiation and influence osteoporosis (OP). The aim of this study was to determine the potential role of miR-874-3p in OP. The expression levels of miR-874-3p and leptin (LEP) in the femoral neck trabeculae of 35 patients with or without OP were measured b...

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Autores principales:	Ling Mei, Min Li, Tao Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	osteoblast differentiation hbmscs mir-874-3p lep osteoporosis Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/ea4152c9b1494de68d2ca9ed0f62137f
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spelling	oai:doaj.org-article:ea4152c9b1494de68d2ca9ed0f62137f2021-11-26T11:19:50ZMicroRNA miR-874-3p inhibits osteoporosis by targeting leptin (LEP)2165-59792165-598710.1080/21655979.2021.2009618https://doaj.org/article/ea4152c9b1494de68d2ca9ed0f62137f2021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2009618https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987MicroRNAs (miRNAs) regulate osteogenic differentiation and influence osteoporosis (OP). The aim of this study was to determine the potential role of miR-874-3p in OP. The expression levels of miR-874-3p and leptin (LEP) in the femoral neck trabeculae of 35 patients with or without OP were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The effects of miR-874-3p or LEP on the cell proliferation and alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), and osterix (OSX) levels were observed by upregulating miR-874-3p in human bone marrow mesenchymal stem cells (hBMSCs). Additionally, calcium deposition levels were evaluated using alizarin red staining (ARS). Molecular mechanisms of miR-874-3p and LEP underlying the osteogenic differentiation of hBMSCs were also evaluated using bioinformatics analysis, luciferase reporter assays, and RNA pull-down assays. The miR-874-3p levels were significantly lower in the femoral neck trabeculae of patients with OP than those of the control group, while the opposite was observed regarding the levels of LEP. Expression levels of miR-874-3p in hBMSCs were upregulated during osteogenic differentiation, while those of LEP were downregulated. Moreover, miR-874-3p upregulation promoted ALP, RUNX2, OCN, and OSX mRNA expression, cell proliferation, and calcium deposition in hBMSCs. LEP was found to be a target gene of miR-874-3p. Overexpression of LEP inhibited the expression of osteoblast markers and reversed the effect of osteogenic differentiation induced by the upregulation of miR-874-3p. In conclusion, miR-874-3p promoted the proliferation and differentiation of hBMSCs by downregulating the expression of LEP, thus inhibiting OP.Ling MeiMin LiTao ZhangTaylor & Francis Grouparticleosteoblast differentiationhbmscsmir-874-3pleposteoporosisBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	osteoblast differentiation hbmscs mir-874-3p lep osteoporosis Biotechnology TP248.13-248.65
spellingShingle	osteoblast differentiation hbmscs mir-874-3p lep osteoporosis Biotechnology TP248.13-248.65 Ling Mei Min Li Tao Zhang MicroRNA miR-874-3p inhibits osteoporosis by targeting leptin (LEP)
description	MicroRNAs (miRNAs) regulate osteogenic differentiation and influence osteoporosis (OP). The aim of this study was to determine the potential role of miR-874-3p in OP. The expression levels of miR-874-3p and leptin (LEP) in the femoral neck trabeculae of 35 patients with or without OP were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The effects of miR-874-3p or LEP on the cell proliferation and alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), and osterix (OSX) levels were observed by upregulating miR-874-3p in human bone marrow mesenchymal stem cells (hBMSCs). Additionally, calcium deposition levels were evaluated using alizarin red staining (ARS). Molecular mechanisms of miR-874-3p and LEP underlying the osteogenic differentiation of hBMSCs were also evaluated using bioinformatics analysis, luciferase reporter assays, and RNA pull-down assays. The miR-874-3p levels were significantly lower in the femoral neck trabeculae of patients with OP than those of the control group, while the opposite was observed regarding the levels of LEP. Expression levels of miR-874-3p in hBMSCs were upregulated during osteogenic differentiation, while those of LEP were downregulated. Moreover, miR-874-3p upregulation promoted ALP, RUNX2, OCN, and OSX mRNA expression, cell proliferation, and calcium deposition in hBMSCs. LEP was found to be a target gene of miR-874-3p. Overexpression of LEP inhibited the expression of osteoblast markers and reversed the effect of osteogenic differentiation induced by the upregulation of miR-874-3p. In conclusion, miR-874-3p promoted the proliferation and differentiation of hBMSCs by downregulating the expression of LEP, thus inhibiting OP.
format	article
author	Ling Mei Min Li Tao Zhang
author_facet	Ling Mei Min Li Tao Zhang
author_sort	Ling Mei
title	MicroRNA miR-874-3p inhibits osteoporosis by targeting leptin (LEP)
title_short	MicroRNA miR-874-3p inhibits osteoporosis by targeting leptin (LEP)
title_full	MicroRNA miR-874-3p inhibits osteoporosis by targeting leptin (LEP)
title_fullStr	MicroRNA miR-874-3p inhibits osteoporosis by targeting leptin (LEP)
title_full_unstemmed	MicroRNA miR-874-3p inhibits osteoporosis by targeting leptin (LEP)
title_sort	microrna mir-874-3p inhibits osteoporosis by targeting leptin (lep)
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/ea4152c9b1494de68d2ca9ed0f62137f
work_keys_str_mv	AT lingmei micrornamir8743pinhibitsosteoporosisbytargetingleptinlep AT minli micrornamir8743pinhibitsosteoporosisbytargetingleptinlep AT taozhang micrornamir8743pinhibitsosteoporosisbytargetingleptinlep
_version_	1718409505706868736

MicroRNA miR-874-3p inhibits osteoporosis by targeting leptin (LEP)

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