The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats

The anti-inflammatory and cholesterol lowering capabilities of probiotic bacteria highlight them as potential prophylactics against chronic inflammatory diseases, particularly cardiovascular disease. Previous studies in silico, in vitro, and in vivo suggest that the Lab4 probiotic consortium may har...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Thomas S. Webberley, Giulia Masetti, Laura M. Baker, Jordanna Dally, Timothy R. Hughes, Julian R. Marchesi, Alison A. Jack, Sue F. Plummer, Guru Ramanathan, Paul D. Facey, Daryn R. Michael
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	probiotic inflammation SCFA cholesterol bile cardiovascular Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/ea4801e0729649bd94e425beaf15c7f5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ea4801e0729649bd94e425beaf15c7f5
record_format	dspace
spelling	oai:doaj.org-article:ea4801e0729649bd94e425beaf15c7f52021-12-01T01:24:00ZThe Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats2296-861X10.3389/fnut.2021.778289https://doaj.org/article/ea4801e0729649bd94e425beaf15c7f52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnut.2021.778289/fullhttps://doaj.org/toc/2296-861XThe anti-inflammatory and cholesterol lowering capabilities of probiotic bacteria highlight them as potential prophylactics against chronic inflammatory diseases, particularly cardiovascular disease. Previous studies in silico, in vitro, and in vivo suggest that the Lab4 probiotic consortium may harbour such capabilities and in the current study, we assessed plasma levels of cytokines/chemokines, short chain fatty acids and lipids and faecal levels of bile acids in a subpopulation of healthy Wistar rats included in 90-day repeat dose oral toxicity study. In the rats receiving Lab4, circulating levels of pro-inflammatory interleukin-6, tumour necrosis factor-α and keratinocyte chemoattractant/growth regulated oncogene were significantly lower compared to the control group demonstrating a systemic anti-inflammatory effect. These changes occurred alongside significant reductions in plasma low density lipoprotein cholesterol and increases in faecal bile acid excretion implying the ability to lower circulating cholesterol via the deconjugation of intestinal bile acids. Correlative analysis identified significant associations between plasma tumour necrosis factor-α and the plasma total cholesterol:high density lipoprotein cholesterol ratio and faecal levels of bifidobacteria in the Lab4 rats. Together, these data highlight Lab4 supplementation as a holistic approach to CVD prevention and encourages further studies in humans.Thomas S. WebberleyGiulia MasettiLaura M. BakerJordanna DallyTimothy R. HughesJulian R. MarchesiAlison A. JackSue F. PlummerGuru RamanathanPaul D. FaceyDaryn R. MichaelFrontiers Media S.A.articleprobioticinflammationSCFAcholesterolbilecardiovascularNutrition. Foods and food supplyTX341-641ENFrontiers in Nutrition, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	probiotic inflammation SCFA cholesterol bile cardiovascular Nutrition. Foods and food supply TX341-641
spellingShingle	probiotic inflammation SCFA cholesterol bile cardiovascular Nutrition. Foods and food supply TX341-641 Thomas S. Webberley Giulia Masetti Laura M. Baker Jordanna Dally Timothy R. Hughes Julian R. Marchesi Alison A. Jack Sue F. Plummer Guru Ramanathan Paul D. Facey Daryn R. Michael The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats
description	The anti-inflammatory and cholesterol lowering capabilities of probiotic bacteria highlight them as potential prophylactics against chronic inflammatory diseases, particularly cardiovascular disease. Previous studies in silico, in vitro, and in vivo suggest that the Lab4 probiotic consortium may harbour such capabilities and in the current study, we assessed plasma levels of cytokines/chemokines, short chain fatty acids and lipids and faecal levels of bile acids in a subpopulation of healthy Wistar rats included in 90-day repeat dose oral toxicity study. In the rats receiving Lab4, circulating levels of pro-inflammatory interleukin-6, tumour necrosis factor-α and keratinocyte chemoattractant/growth regulated oncogene were significantly lower compared to the control group demonstrating a systemic anti-inflammatory effect. These changes occurred alongside significant reductions in plasma low density lipoprotein cholesterol and increases in faecal bile acid excretion implying the ability to lower circulating cholesterol via the deconjugation of intestinal bile acids. Correlative analysis identified significant associations between plasma tumour necrosis factor-α and the plasma total cholesterol:high density lipoprotein cholesterol ratio and faecal levels of bifidobacteria in the Lab4 rats. Together, these data highlight Lab4 supplementation as a holistic approach to CVD prevention and encourages further studies in humans.
format	article
author	Thomas S. Webberley Giulia Masetti Laura M. Baker Jordanna Dally Timothy R. Hughes Julian R. Marchesi Alison A. Jack Sue F. Plummer Guru Ramanathan Paul D. Facey Daryn R. Michael
author_facet	Thomas S. Webberley Giulia Masetti Laura M. Baker Jordanna Dally Timothy R. Hughes Julian R. Marchesi Alison A. Jack Sue F. Plummer Guru Ramanathan Paul D. Facey Daryn R. Michael
author_sort	Thomas S. Webberley
title	The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats
title_short	The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats
title_full	The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats
title_fullStr	The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats
title_full_unstemmed	The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats
title_sort	impact of lab4 probiotic supplementation in a 90-day study in wistar rats
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ea4801e0729649bd94e425beaf15c7f5
work_keys_str_mv	AT thomasswebberley theimpactoflab4probioticsupplementationina90daystudyinwistarrats AT giuliamasetti theimpactoflab4probioticsupplementationina90daystudyinwistarrats AT laurambaker theimpactoflab4probioticsupplementationina90daystudyinwistarrats AT jordannadally theimpactoflab4probioticsupplementationina90daystudyinwistarrats AT timothyrhughes theimpactoflab4probioticsupplementationina90daystudyinwistarrats AT julianrmarchesi theimpactoflab4probioticsupplementationina90daystudyinwistarrats AT alisonajack theimpactoflab4probioticsupplementationina90daystudyinwistarrats AT suefplummer theimpactoflab4probioticsupplementationina90daystudyinwistarrats AT gururamanathan theimpactoflab4probioticsupplementationina90daystudyinwistarrats AT pauldfacey theimpactoflab4probioticsupplementationina90daystudyinwistarrats AT darynrmichael theimpactoflab4probioticsupplementationina90daystudyinwistarrats AT thomasswebberley impactoflab4probioticsupplementationina90daystudyinwistarrats AT giuliamasetti impactoflab4probioticsupplementationina90daystudyinwistarrats AT laurambaker impactoflab4probioticsupplementationina90daystudyinwistarrats AT jordannadally impactoflab4probioticsupplementationina90daystudyinwistarrats AT timothyrhughes impactoflab4probioticsupplementationina90daystudyinwistarrats AT julianrmarchesi impactoflab4probioticsupplementationina90daystudyinwistarrats AT alisonajack impactoflab4probioticsupplementationina90daystudyinwistarrats AT suefplummer impactoflab4probioticsupplementationina90daystudyinwistarrats AT gururamanathan impactoflab4probioticsupplementationina90daystudyinwistarrats AT pauldfacey impactoflab4probioticsupplementationina90daystudyinwistarrats AT darynrmichael impactoflab4probioticsupplementationina90daystudyinwistarrats
_version_	1718405994582638592

The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats

Ejemplares similares