Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes

Culturing transposon-mutant libraries in pools can mask complex phenotypes. Here the authors present microfluidics mediated droplet Tn-Seq, which encapsulates individual mutants, promotes isolated growth and enables cell-cell interaction analyses.

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Detalles Bibliográficos
Autores principales:	Derek Thibault, Paul A. Jensen, Stephen Wood, Christine Qabar, Stacie Clark, Mara G. Shainheit, Ralph R. Isberg, Tim van Opijnen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ea4ad1594e3847579e9ba764776164b2
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spelling	oai:doaj.org-article:ea4ad1594e3847579e9ba764776164b22021-12-02T16:57:07ZDroplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes10.1038/s41467-019-13719-92041-1723https://doaj.org/article/ea4ad1594e3847579e9ba764776164b22019-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13719-9https://doaj.org/toc/2041-1723Culturing transposon-mutant libraries in pools can mask complex phenotypes. Here the authors present microfluidics mediated droplet Tn-Seq, which encapsulates individual mutants, promotes isolated growth and enables cell-cell interaction analyses.Derek ThibaultPaul A. JensenStephen WoodChristine QabarStacie ClarkMara G. ShainheitRalph R. IsbergTim van OpijnenNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Derek Thibault Paul A. Jensen Stephen Wood Christine Qabar Stacie Clark Mara G. Shainheit Ralph R. Isberg Tim van Opijnen Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes
description	Culturing transposon-mutant libraries in pools can mask complex phenotypes. Here the authors present microfluidics mediated droplet Tn-Seq, which encapsulates individual mutants, promotes isolated growth and enables cell-cell interaction analyses.
format	article
author	Derek Thibault Paul A. Jensen Stephen Wood Christine Qabar Stacie Clark Mara G. Shainheit Ralph R. Isberg Tim van Opijnen
author_facet	Derek Thibault Paul A. Jensen Stephen Wood Christine Qabar Stacie Clark Mara G. Shainheit Ralph R. Isberg Tim van Opijnen
author_sort	Derek Thibault
title	Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes
title_short	Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes
title_full	Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes
title_fullStr	Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes
title_full_unstemmed	Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes
title_sort	droplet tn-seq combines microfluidics with tn-seq for identifying complex single-cell phenotypes
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/ea4ad1594e3847579e9ba764776164b2
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Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes

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