Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes

Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes

Culturing transposon-mutant libraries in pools can mask complex phenotypes. Here the authors present microfluidics mediated droplet Tn-Seq, which encapsulates individual mutants, promotes isolated growth and enables cell-cell interaction analyses.

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Detalles Bibliográficos
Autores principales: Derek Thibault, Paul A. Jensen, Stephen Wood, Christine Qabar, Stacie Clark, Mara G. Shainheit, Ralph R. Isberg, Tim van Opijnen
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
Materias:
Science
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Acceso en línea:https://doaj.org/article/ea4ad1594e3847579e9ba764776164b2
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