Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes
Culturing transposon-mutant libraries in pools can mask complex phenotypes. Here the authors present microfluidics mediated droplet Tn-Seq, which encapsulates individual mutants, promotes isolated growth and enables cell-cell interaction analyses.
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Auteurs principaux: | Derek Thibault, Paul A. Jensen, Stephen Wood, Christine Qabar, Stacie Clark, Mara G. Shainheit, Ralph R. Isberg, Tim van Opijnen |
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Format: | article |
Langue: | EN |
Publié: |
Nature Portfolio
2019
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Accès en ligne: | https://doaj.org/article/ea4ad1594e3847579e9ba764776164b2 |
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