Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells

Abstract Human pluripotent stem cells are a potentially powerful cellular resource for application in regenerative medicine. Because such applications require large numbers of human pluripotent stem cell-derived cells, a scalable culture system of human pluripotent stem cell needs to be developed. S...

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Autores principales:	Kazuhiro Ikeda, Shogo Nagata, Teru Okitsu, Shoji Takeuchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ea4e09456b9f49ba84a24c8902e06b26
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spelling	oai:doaj.org-article:ea4e09456b9f49ba84a24c8902e06b262021-12-02T16:06:02ZCell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells10.1038/s41598-017-03246-22045-2322https://doaj.org/article/ea4e09456b9f49ba84a24c8902e06b262017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03246-2https://doaj.org/toc/2045-2322Abstract Human pluripotent stem cells are a potentially powerful cellular resource for application in regenerative medicine. Because such applications require large numbers of human pluripotent stem cell-derived cells, a scalable culture system of human pluripotent stem cell needs to be developed. Several suspension culture systems for human pluripotent stem cell expansion exist; however, it is difficult to control the thickness of cell aggregations in these systems, leading to increased cell death likely caused by limited diffusion of gases and nutrients into the aggregations. Here, we describe a scalable culture system using the cell fiber technology for the expansion of human induced pluripotent stem (iPS) cells. The cells were encapsulated and cultured within the core region of core-shell hydrogel microfibers, resulting in the formation of rod-shaped or fiber-shaped cell aggregations with sustained thickness and high viability. By encapsulating the cells with type I collagen, we demonstrated a long-term culture of the cells by serial passaging at a high expansion rate (14-fold in four days) while retaining its pluripotency. Therefore, our culture system could be used for large-scale expansion of human pluripotent stem cells for use in regenerative medicine.Kazuhiro IkedaShogo NagataTeru OkitsuShoji TakeuchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kazuhiro Ikeda Shogo Nagata Teru Okitsu Shoji Takeuchi Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells
description	Abstract Human pluripotent stem cells are a potentially powerful cellular resource for application in regenerative medicine. Because such applications require large numbers of human pluripotent stem cell-derived cells, a scalable culture system of human pluripotent stem cell needs to be developed. Several suspension culture systems for human pluripotent stem cell expansion exist; however, it is difficult to control the thickness of cell aggregations in these systems, leading to increased cell death likely caused by limited diffusion of gases and nutrients into the aggregations. Here, we describe a scalable culture system using the cell fiber technology for the expansion of human induced pluripotent stem (iPS) cells. The cells were encapsulated and cultured within the core region of core-shell hydrogel microfibers, resulting in the formation of rod-shaped or fiber-shaped cell aggregations with sustained thickness and high viability. By encapsulating the cells with type I collagen, we demonstrated a long-term culture of the cells by serial passaging at a high expansion rate (14-fold in four days) while retaining its pluripotency. Therefore, our culture system could be used for large-scale expansion of human pluripotent stem cells for use in regenerative medicine.
format	article
author	Kazuhiro Ikeda Shogo Nagata Teru Okitsu Shoji Takeuchi
author_facet	Kazuhiro Ikeda Shogo Nagata Teru Okitsu Shoji Takeuchi
author_sort	Kazuhiro Ikeda
title	Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells
title_short	Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells
title_full	Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells
title_fullStr	Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells
title_full_unstemmed	Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells
title_sort	cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ea4e09456b9f49ba84a24c8902e06b26
work_keys_str_mv	AT kazuhiroikeda cellfiberbasedthreedimensionalculturesystemforhighlyefficientexpansionofhumaninducedpluripotentstemcells AT shogonagata cellfiberbasedthreedimensionalculturesystemforhighlyefficientexpansionofhumaninducedpluripotentstemcells AT teruokitsu cellfiberbasedthreedimensionalculturesystemforhighlyefficientexpansionofhumaninducedpluripotentstemcells AT shojitakeuchi cellfiberbasedthreedimensionalculturesystemforhighlyefficientexpansionofhumaninducedpluripotentstemcells
_version_	1718385182282612736

Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells

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