Visualizing the in-vivo application of zinc in sensitive skin using reflectance confocal microscopy

Abstract Findings obtained on objective assessments to evaluate sensitive skin do not correlate well with the symptomatology. We utilized reflectance confocal microscopy (RCM) to compare transepidermal application of zinc in sensitive and non-sensitive skin. Thirty-six subjects participated in this...

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Autores principales:	Hye-Jin Ahn, Hae Jin Kim, Hyein Ham, Ji Hwoon Baek, Young Lee, Mahin Alamgir, Babar Rao, Min Kyung Shin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ea5868ad0bd74de9b06c5dc410ac682a
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spelling	oai:doaj.org-article:ea5868ad0bd74de9b06c5dc410ac682a2021-12-02T14:37:46ZVisualizing the in-vivo application of zinc in sensitive skin using reflectance confocal microscopy10.1038/s41598-021-87346-02045-2322https://doaj.org/article/ea5868ad0bd74de9b06c5dc410ac682a2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87346-0https://doaj.org/toc/2045-2322Abstract Findings obtained on objective assessments to evaluate sensitive skin do not correlate well with the symptomatology. We utilized reflectance confocal microscopy (RCM) to compare transepidermal application of zinc in sensitive and non-sensitive skin. Thirty-six subjects participated in this study. They were divided into groups based on lactic acid sting test (LAST):‘stinger’ and ‘non-stinger’; transepidermal water loss (TEWL) measurements; and sensitivity self-assessments: ‘sensitive’ and ‘non-sensitive’. RCM images were taken to visualize transepidermal application of topically-applied zinc. The intensity of zinc reflectance at different depths was measured by ImageJ software. Based on LAST scores, the ‘stinger’ group showed significantly higher reflectance of zinc at 8 µm (stratum corneum) [face (P < 0.001), forearm (P = 0.004)], and at 80–104 µm (dermo-epidermal junction layer) on the face. High-TEWL group showed increased zinc reflectance at 8–24 µm (tight junction layer, P < 0.001). There were no significant differences amongst subjects self-reporting ‘sensitive’ and ‘non-sensitive’ skin. RCM demonstrates that in sensitive skin, there is deeper and higher reflectance of zinc at multiple depths. Structural differences are also visualized. We suggest that RCM is a useful tool for evaluating skin barrier integrity.Hye-Jin AhnHae Jin KimHyein HamJi Hwoon BaekYoung LeeMahin AlamgirBabar RaoMin Kyung ShinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hye-Jin Ahn Hae Jin Kim Hyein Ham Ji Hwoon Baek Young Lee Mahin Alamgir Babar Rao Min Kyung Shin Visualizing the in-vivo application of zinc in sensitive skin using reflectance confocal microscopy
description	Abstract Findings obtained on objective assessments to evaluate sensitive skin do not correlate well with the symptomatology. We utilized reflectance confocal microscopy (RCM) to compare transepidermal application of zinc in sensitive and non-sensitive skin. Thirty-six subjects participated in this study. They were divided into groups based on lactic acid sting test (LAST):‘stinger’ and ‘non-stinger’; transepidermal water loss (TEWL) measurements; and sensitivity self-assessments: ‘sensitive’ and ‘non-sensitive’. RCM images were taken to visualize transepidermal application of topically-applied zinc. The intensity of zinc reflectance at different depths was measured by ImageJ software. Based on LAST scores, the ‘stinger’ group showed significantly higher reflectance of zinc at 8 µm (stratum corneum) [face (P < 0.001), forearm (P = 0.004)], and at 80–104 µm (dermo-epidermal junction layer) on the face. High-TEWL group showed increased zinc reflectance at 8–24 µm (tight junction layer, P < 0.001). There were no significant differences amongst subjects self-reporting ‘sensitive’ and ‘non-sensitive’ skin. RCM demonstrates that in sensitive skin, there is deeper and higher reflectance of zinc at multiple depths. Structural differences are also visualized. We suggest that RCM is a useful tool for evaluating skin barrier integrity.
format	article
author	Hye-Jin Ahn Hae Jin Kim Hyein Ham Ji Hwoon Baek Young Lee Mahin Alamgir Babar Rao Min Kyung Shin
author_facet	Hye-Jin Ahn Hae Jin Kim Hyein Ham Ji Hwoon Baek Young Lee Mahin Alamgir Babar Rao Min Kyung Shin
author_sort	Hye-Jin Ahn
title	Visualizing the in-vivo application of zinc in sensitive skin using reflectance confocal microscopy
title_short	Visualizing the in-vivo application of zinc in sensitive skin using reflectance confocal microscopy
title_full	Visualizing the in-vivo application of zinc in sensitive skin using reflectance confocal microscopy
title_fullStr	Visualizing the in-vivo application of zinc in sensitive skin using reflectance confocal microscopy
title_full_unstemmed	Visualizing the in-vivo application of zinc in sensitive skin using reflectance confocal microscopy
title_sort	visualizing the in-vivo application of zinc in sensitive skin using reflectance confocal microscopy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ea5868ad0bd74de9b06c5dc410ac682a
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Visualizing the in-vivo application of zinc in sensitive skin using reflectance confocal microscopy

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