High salt diet impairs dermal tissue remodeling in a mouse model of IMQ induced dermatitis.

Recent animal studies, as well as quantitative sodium MRI observations on humans demonstrated that remarkable amounts of sodium can be stored in the skin. It is also known that excess sodium in the tissues leads to inflammation in various organs, but its role in dermal pathophysiology has not been e...

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Autores principales:	Csenge Pajtók, Apor Veres-Székely, Róbert Agócs, Beáta Szebeni, Péter Dobosy, István Németh, Zoltán Veréb, Lajos Kemény, Attila J Szabó, Ádám Vannay, Tivadar Tulassay, Domonkos Pap
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ea5f354fcdf5445b850f5f0530352e59
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spelling	oai:doaj.org-article:ea5f354fcdf5445b850f5f0530352e592021-12-02T20:04:36ZHigh salt diet impairs dermal tissue remodeling in a mouse model of IMQ induced dermatitis.1932-620310.1371/journal.pone.0258502https://doaj.org/article/ea5f354fcdf5445b850f5f0530352e592021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258502https://doaj.org/toc/1932-6203Recent animal studies, as well as quantitative sodium MRI observations on humans demonstrated that remarkable amounts of sodium can be stored in the skin. It is also known that excess sodium in the tissues leads to inflammation in various organs, but its role in dermal pathophysiology has not been elucidated. Therefore, our aim was to study the effect of dietary salt loading on inflammatory process and related extracellular matrix (ECM) remodeling in the skin. To investigate the effect of high salt consumption on inflammation and ECM production in the skin mice were kept on normal (NSD) or high salt (HSD) diet and then dermatitis was induced with imiquimod (IMQ) treatment. The effect of high salt concentration on dermal fibroblasts (DF) and peripheral blood mononuclear cells (PBMC) was also investigated in vitro. The HSD resulted in increased sodium content in the skin of mice. Inflammatory cytokine Il17 expression was elevated in the skin of HSD mice. Expression of anti-inflammatory Il10 and Il13 decreased in the skin of HSD or HSD IMQ mice. The fibroblast marker Acta2 and ECM component Fn and Col1a1 decreased in HSD IMQ mice. Expression of ECM remodeling related Pdgfb and activation phosphorylated (p)-SMAD2/3 was lower in HSD IMQ mice. In PBMCs, production of IL10, IL13 and PDGFB was reduced due to high salt loading. In cultured DFs high salt concentration resulted in decreased cell motility and ECM production, as well. Our results demonstrate that high dietary salt intake is associated with increased dermal pro-inflammatory status. Interestingly, although inflammation induces the synthesis of ECM in most organs, the expression of ECM decreased in the inflamed skin of mice on high salt diet. Our data suggest that salt intake may alter the process of skin remodeling.Csenge PajtókApor Veres-SzékelyRóbert AgócsBeáta SzebeniPéter DobosyIstván NémethZoltán VerébLajos KeményAttila J SzabóÁdám VannayTivadar TulassayDomonkos PapPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0258502 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Csenge Pajtók Apor Veres-Székely Róbert Agócs Beáta Szebeni Péter Dobosy István Németh Zoltán Veréb Lajos Kemény Attila J Szabó Ádám Vannay Tivadar Tulassay Domonkos Pap High salt diet impairs dermal tissue remodeling in a mouse model of IMQ induced dermatitis.
description	Recent animal studies, as well as quantitative sodium MRI observations on humans demonstrated that remarkable amounts of sodium can be stored in the skin. It is also known that excess sodium in the tissues leads to inflammation in various organs, but its role in dermal pathophysiology has not been elucidated. Therefore, our aim was to study the effect of dietary salt loading on inflammatory process and related extracellular matrix (ECM) remodeling in the skin. To investigate the effect of high salt consumption on inflammation and ECM production in the skin mice were kept on normal (NSD) or high salt (HSD) diet and then dermatitis was induced with imiquimod (IMQ) treatment. The effect of high salt concentration on dermal fibroblasts (DF) and peripheral blood mononuclear cells (PBMC) was also investigated in vitro. The HSD resulted in increased sodium content in the skin of mice. Inflammatory cytokine Il17 expression was elevated in the skin of HSD mice. Expression of anti-inflammatory Il10 and Il13 decreased in the skin of HSD or HSD IMQ mice. The fibroblast marker Acta2 and ECM component Fn and Col1a1 decreased in HSD IMQ mice. Expression of ECM remodeling related Pdgfb and activation phosphorylated (p)-SMAD2/3 was lower in HSD IMQ mice. In PBMCs, production of IL10, IL13 and PDGFB was reduced due to high salt loading. In cultured DFs high salt concentration resulted in decreased cell motility and ECM production, as well. Our results demonstrate that high dietary salt intake is associated with increased dermal pro-inflammatory status. Interestingly, although inflammation induces the synthesis of ECM in most organs, the expression of ECM decreased in the inflamed skin of mice on high salt diet. Our data suggest that salt intake may alter the process of skin remodeling.
format	article
author	Csenge Pajtók Apor Veres-Székely Róbert Agócs Beáta Szebeni Péter Dobosy István Németh Zoltán Veréb Lajos Kemény Attila J Szabó Ádám Vannay Tivadar Tulassay Domonkos Pap
author_facet	Csenge Pajtók Apor Veres-Székely Róbert Agócs Beáta Szebeni Péter Dobosy István Németh Zoltán Veréb Lajos Kemény Attila J Szabó Ádám Vannay Tivadar Tulassay Domonkos Pap
author_sort	Csenge Pajtók
title	High salt diet impairs dermal tissue remodeling in a mouse model of IMQ induced dermatitis.
title_short	High salt diet impairs dermal tissue remodeling in a mouse model of IMQ induced dermatitis.
title_full	High salt diet impairs dermal tissue remodeling in a mouse model of IMQ induced dermatitis.
title_fullStr	High salt diet impairs dermal tissue remodeling in a mouse model of IMQ induced dermatitis.
title_full_unstemmed	High salt diet impairs dermal tissue remodeling in a mouse model of IMQ induced dermatitis.
title_sort	high salt diet impairs dermal tissue remodeling in a mouse model of imq induced dermatitis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ea5f354fcdf5445b850f5f0530352e59
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High salt diet impairs dermal tissue remodeling in a mouse model of IMQ induced dermatitis.

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