Effects of Adding Laccase to Bacterial Consortia Degrading Heavy Oil

High-efficiency bioremediation technology for heavy oil pollution has been a popular research topic in recent years. Laccase is very promising for the remediation of heavy oil pollution because it can not only convert bio-refractory hydrocarbons into less toxic or completely harmless compounds, but...

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Autores principales:	Xiaoli Dai, Jing Lv, Wenxia Wei, Shaohui Guo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	heavy oil pollution bioremediation laccase bacterial consortia degradation Chemical technology TP1-1185 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/ea5fe9f0b40c4acebf6af4b89a8ef850
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spelling	oai:doaj.org-article:ea5fe9f0b40c4acebf6af4b89a8ef8502021-11-25T18:51:34ZEffects of Adding Laccase to Bacterial Consortia Degrading Heavy Oil10.3390/pr91120252227-9717https://doaj.org/article/ea5fe9f0b40c4acebf6af4b89a8ef8502021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9717/9/11/2025https://doaj.org/toc/2227-9717High-efficiency bioremediation technology for heavy oil pollution has been a popular research topic in recent years. Laccase is very promising for the remediation of heavy oil pollution because it can not only convert bio-refractory hydrocarbons into less toxic or completely harmless compounds, but also accelerate the biodegradation efficiency of heavy oil. However, there are few reports on the use of laccase to enhance the biodegradation of heavy oil. In this study, we investigated the effect of laccase on the bacterial consortia degradation of heavy oil. The degradation efficiencies of bacterial consortia and the laccase-bacterial consortia were 60.6 ± 0.1% and 68.2 ± 0.6%, respectively, and the corresponding heavy oil degradation rate constants were 0.112 day<sup>−1</sup> and 0.198 day<sup>−1</sup>, respectively. The addition of laccase increased the heavy oil biodegradation efficiency (<i>p</i> < 0.05) and biodegradation rate of the bacterial consortia. Moreover, gas chromatography–mass spectrometry analysis showed that the biodegradation efficiencies of the laccase-bacterial consortia for saturated hydrocarbons and aromatic hydrocarbons were 82.5 ± 0.7% and 76.2 ± 0.9%, respectively, which were 16.0 ± 0.3% and 13.0 ± 1.8% higher than those of the bacterial consortia, respectively. In addition, the degradation rate constants of the laccase-bacterial consortia for saturated hydrocarbons and aromatic hydrocarbons were 0.267 day<sup>−1</sup> and 0.226 day<sup>−1</sup>, respectively, which were 1.07 and 1.15 times higher than those of the bacterial consortia, respectively. The degradation of C15 to C35 n-alkanes and 2 to 5-ring polycyclic aromatic hydrocarbons by laccase-bacterial consortia was higher than individual bacterial consortia. It is further seen that the addition of laccase significantly improved the biodegradation of long-chain n-alkanes of C22–C35 (<i>p</i> < 0.05). Overall, this study shows that the combination of laccase and bacterial consortia is an effective remediation technology for heavy oil pollution. Adding laccase can significantly improve the heavy oil biodegradation efficiency and biodegradation rate of the bacterial consortia.Xiaoli DaiJing LvWenxia WeiShaohui GuoMDPI AGarticleheavy oil pollutionbioremediationlaccasebacterial consortiadegradationChemical technologyTP1-1185ChemistryQD1-999ENProcesses, Vol 9, Iss 2025, p 2025 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	heavy oil pollution bioremediation laccase bacterial consortia degradation Chemical technology TP1-1185 Chemistry QD1-999
spellingShingle	heavy oil pollution bioremediation laccase bacterial consortia degradation Chemical technology TP1-1185 Chemistry QD1-999 Xiaoli Dai Jing Lv Wenxia Wei Shaohui Guo Effects of Adding Laccase to Bacterial Consortia Degrading Heavy Oil
description	High-efficiency bioremediation technology for heavy oil pollution has been a popular research topic in recent years. Laccase is very promising for the remediation of heavy oil pollution because it can not only convert bio-refractory hydrocarbons into less toxic or completely harmless compounds, but also accelerate the biodegradation efficiency of heavy oil. However, there are few reports on the use of laccase to enhance the biodegradation of heavy oil. In this study, we investigated the effect of laccase on the bacterial consortia degradation of heavy oil. The degradation efficiencies of bacterial consortia and the laccase-bacterial consortia were 60.6 ± 0.1% and 68.2 ± 0.6%, respectively, and the corresponding heavy oil degradation rate constants were 0.112 day<sup>−1</sup> and 0.198 day<sup>−1</sup>, respectively. The addition of laccase increased the heavy oil biodegradation efficiency (<i>p</i> < 0.05) and biodegradation rate of the bacterial consortia. Moreover, gas chromatography–mass spectrometry analysis showed that the biodegradation efficiencies of the laccase-bacterial consortia for saturated hydrocarbons and aromatic hydrocarbons were 82.5 ± 0.7% and 76.2 ± 0.9%, respectively, which were 16.0 ± 0.3% and 13.0 ± 1.8% higher than those of the bacterial consortia, respectively. In addition, the degradation rate constants of the laccase-bacterial consortia for saturated hydrocarbons and aromatic hydrocarbons were 0.267 day<sup>−1</sup> and 0.226 day<sup>−1</sup>, respectively, which were 1.07 and 1.15 times higher than those of the bacterial consortia, respectively. The degradation of C15 to C35 n-alkanes and 2 to 5-ring polycyclic aromatic hydrocarbons by laccase-bacterial consortia was higher than individual bacterial consortia. It is further seen that the addition of laccase significantly improved the biodegradation of long-chain n-alkanes of C22–C35 (<i>p</i> < 0.05). Overall, this study shows that the combination of laccase and bacterial consortia is an effective remediation technology for heavy oil pollution. Adding laccase can significantly improve the heavy oil biodegradation efficiency and biodegradation rate of the bacterial consortia.
format	article
author	Xiaoli Dai Jing Lv Wenxia Wei Shaohui Guo
author_facet	Xiaoli Dai Jing Lv Wenxia Wei Shaohui Guo
author_sort	Xiaoli Dai
title	Effects of Adding Laccase to Bacterial Consortia Degrading Heavy Oil
title_short	Effects of Adding Laccase to Bacterial Consortia Degrading Heavy Oil
title_full	Effects of Adding Laccase to Bacterial Consortia Degrading Heavy Oil
title_fullStr	Effects of Adding Laccase to Bacterial Consortia Degrading Heavy Oil
title_full_unstemmed	Effects of Adding Laccase to Bacterial Consortia Degrading Heavy Oil
title_sort	effects of adding laccase to bacterial consortia degrading heavy oil
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ea5fe9f0b40c4acebf6af4b89a8ef850
work_keys_str_mv	AT xiaolidai effectsofaddinglaccasetobacterialconsortiadegradingheavyoil AT jinglv effectsofaddinglaccasetobacterialconsortiadegradingheavyoil AT wenxiawei effectsofaddinglaccasetobacterialconsortiadegradingheavyoil AT shaohuiguo effectsofaddinglaccasetobacterialconsortiadegradingheavyoil
_version_	1718410685773250560

Effects of Adding Laccase to Bacterial Consortia Degrading Heavy Oil

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