Development of a liposome-based formulation for vitamin K1 nebulization on the skin

Virginia Campani,1,2 Dario Marchese,1 Maria Teresa Pitaro,2 Michele Pitaro,2 Paolo Grieco,1 Giuseppe De Rosa11Department of Pharmacy, Università degli Studi di Napoli Federico II, Naples, Italy; 2Xenus Srl, Rome, ItalyAbstract: Vitamin K1 (VK1) is a very lipophilic and photosensitive mol...

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Autores principales:	Campani V, Marchese D, Pitaro MT, Pitaro M, Grieco P, De Rosa G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ea6aa67eb88b4abebe345e558e5251c4
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spelling	oai:doaj.org-article:ea6aa67eb88b4abebe345e558e5251c42021-12-02T00:07:20ZDevelopment of a liposome-based formulation for vitamin K1 nebulization on the skin1178-2013https://doaj.org/article/ea6aa67eb88b4abebe345e558e5251c42014-04-01T00:00:00Zhttp://www.dovepress.com/development-of-a-liposome-based-formulation-for-vitamin-k1-nebulizatio-a16424https://doaj.org/toc/1178-2013 Virginia Campani,1,2 Dario Marchese,1 Maria Teresa Pitaro,2 Michele Pitaro,2 Paolo Grieco,1 Giuseppe De Rosa11Department of Pharmacy, Università degli Studi di Napoli Federico II, Naples, Italy; 2Xenus Srl, Rome, ItalyAbstract: Vitamin K1 (VK1) is a very lipophilic and photosensitive molecule contained in some vegetables. Recently, the use of VK1 on the skin has been proposed for different pharmaceutical or cosmeceutical applications. In this study, an innovative strategy for the administration of VK1 on the skin was proposed. In particular, to overcome the drawbacks associated with a VK1-containing fatty ointment available on the market, an aqueous formulation suitable to be administered by nebulization was developed. The use of liposomes encapsulating VK1 enabled issues due to the lipophilicity of VK1 to be overcome. Thus, different liposomal formulations, with different VK1 concentrations, were prepared and characterized in terms of size, zeta potential, VK1 encapsulation into liposomes, and stability of the formulations during storage. After a first phase of screening, the selected formulation was tested by a portable device for nebulization. No alteration of the vesicle characteristics following the liposome supply through the nebulizer was found. Finally, permeation studies were carried out on pig-excised skin in Franz cells and the newly developed formulation was compared to a marketed VK1-containing ointment. In this test, an enhanced VK1 accumulation into the skin was found when using nebulized liposomes. In conclusion, in order to administer VK1 on the skin, the newly developed formulation could be a valid alternative to the products available on the market today. In particular, the use of liposomes could facilitate the multiple administrations per day by aerosol, but also increase, compared to a semi-solid preparation, the accumulation of VK1 into the epidermis and dermis.Keywords: liposomes, liposome stability, liposome nebulization, Franz cellsCampani VMarchese DPitaro MTPitaro MGrieco PDe Rosa GDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1823-1832 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Campani V Marchese D Pitaro MT Pitaro M Grieco P De Rosa G Development of a liposome-based formulation for vitamin K1 nebulization on the skin
description	Virginia Campani,1,2 Dario Marchese,1 Maria Teresa Pitaro,2 Michele Pitaro,2 Paolo Grieco,1 Giuseppe De Rosa11Department of Pharmacy, Università degli Studi di Napoli Federico II, Naples, Italy; 2Xenus Srl, Rome, ItalyAbstract: Vitamin K1 (VK1) is a very lipophilic and photosensitive molecule contained in some vegetables. Recently, the use of VK1 on the skin has been proposed for different pharmaceutical or cosmeceutical applications. In this study, an innovative strategy for the administration of VK1 on the skin was proposed. In particular, to overcome the drawbacks associated with a VK1-containing fatty ointment available on the market, an aqueous formulation suitable to be administered by nebulization was developed. The use of liposomes encapsulating VK1 enabled issues due to the lipophilicity of VK1 to be overcome. Thus, different liposomal formulations, with different VK1 concentrations, were prepared and characterized in terms of size, zeta potential, VK1 encapsulation into liposomes, and stability of the formulations during storage. After a first phase of screening, the selected formulation was tested by a portable device for nebulization. No alteration of the vesicle characteristics following the liposome supply through the nebulizer was found. Finally, permeation studies were carried out on pig-excised skin in Franz cells and the newly developed formulation was compared to a marketed VK1-containing ointment. In this test, an enhanced VK1 accumulation into the skin was found when using nebulized liposomes. In conclusion, in order to administer VK1 on the skin, the newly developed formulation could be a valid alternative to the products available on the market today. In particular, the use of liposomes could facilitate the multiple administrations per day by aerosol, but also increase, compared to a semi-solid preparation, the accumulation of VK1 into the epidermis and dermis.Keywords: liposomes, liposome stability, liposome nebulization, Franz cells
format	article
author	Campani V Marchese D Pitaro MT Pitaro M Grieco P De Rosa G
author_facet	Campani V Marchese D Pitaro MT Pitaro M Grieco P De Rosa G
author_sort	Campani V
title	Development of a liposome-based formulation for vitamin K1 nebulization on the skin
title_short	Development of a liposome-based formulation for vitamin K1 nebulization on the skin
title_full	Development of a liposome-based formulation for vitamin K1 nebulization on the skin
title_fullStr	Development of a liposome-based formulation for vitamin K1 nebulization on the skin
title_full_unstemmed	Development of a liposome-based formulation for vitamin K1 nebulization on the skin
title_sort	development of a liposome-based formulation for vitamin k1 nebulization on the skin
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/ea6aa67eb88b4abebe345e558e5251c4
work_keys_str_mv	AT campaniv developmentofaliposomebasedformulationforvitamink1nebulizationontheskin AT marchesed developmentofaliposomebasedformulationforvitamink1nebulizationontheskin AT pitaromt developmentofaliposomebasedformulationforvitamink1nebulizationontheskin AT pitarom developmentofaliposomebasedformulationforvitamink1nebulizationontheskin AT griecop developmentofaliposomebasedformulationforvitamink1nebulizationontheskin AT derosag developmentofaliposomebasedformulationforvitamink1nebulizationontheskin
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Development of a liposome-based formulation for vitamin K1 nebulization on the skin

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