The world of ribonucleases from pseudomonads: a short trip through the main features and singularities

Summary The development of synthetic biology has brought an unprecedented increase in the number molecular tools applicable into a microbial chassis. The exploration of such tools into different bacteria revealed not only the challenges of context dependency of biological functions but also the comp...

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Autores principales:	Patrícia Apura, Luis G. Gonçalves, Sandra C. Viegas, Cecília M. Arraiano
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/ea6e06f0be934d16b320021dbf276563
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spelling	oai:doaj.org-article:ea6e06f0be934d16b320021dbf2765632021-11-18T15:39:52ZThe world of ribonucleases from pseudomonads: a short trip through the main features and singularities1751-791510.1111/1751-7915.13890https://doaj.org/article/ea6e06f0be934d16b320021dbf2765632021-11-01T00:00:00Zhttps://doi.org/10.1111/1751-7915.13890https://doaj.org/toc/1751-7915Summary The development of synthetic biology has brought an unprecedented increase in the number molecular tools applicable into a microbial chassis. The exploration of such tools into different bacteria revealed not only the challenges of context dependency of biological functions but also the complexity and diversity of regulatory layers in bacterial cells. Most of the standardized genetic tools and principles/functions have been mostly based on model microorganisms, namely Escherichia coli. In contrast, the non‐model pseudomonads lack a deeper understanding of their regulatory layers and have limited molecular tools. They are resistant pathogens and promising alternative bacterial chassis, making them attractive targets for further studies. Ribonucleases (RNases) are key players in the post‐transcriptional control of gene expression by degrading or processing the RNA molecules in the cell. These enzymes act according to the cellular requirements and can also be seen as the recyclers of ribonucleotides, allowing a continuous input of these cellular resources. This makes these post‐transcriptional regulators perfect candidates to regulate microbial physiology. This review summarizes the current knowledge and unique properties of ribonucleases in the world of pseudomonads, taking into account genomic context analysis, biological function and strategies to use ribonucleases to improve biotechnological processes.Patrícia ApuraLuis G. GonçalvesSandra C. ViegasCecília M. ArraianoWileyarticleBiotechnologyTP248.13-248.65ENMicrobial Biotechnology, Vol 14, Iss 6, Pp 2316-2333 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65
spellingShingle	Biotechnology TP248.13-248.65 Patrícia Apura Luis G. Gonçalves Sandra C. Viegas Cecília M. Arraiano The world of ribonucleases from pseudomonads: a short trip through the main features and singularities
description	Summary The development of synthetic biology has brought an unprecedented increase in the number molecular tools applicable into a microbial chassis. The exploration of such tools into different bacteria revealed not only the challenges of context dependency of biological functions but also the complexity and diversity of regulatory layers in bacterial cells. Most of the standardized genetic tools and principles/functions have been mostly based on model microorganisms, namely Escherichia coli. In contrast, the non‐model pseudomonads lack a deeper understanding of their regulatory layers and have limited molecular tools. They are resistant pathogens and promising alternative bacterial chassis, making them attractive targets for further studies. Ribonucleases (RNases) are key players in the post‐transcriptional control of gene expression by degrading or processing the RNA molecules in the cell. These enzymes act according to the cellular requirements and can also be seen as the recyclers of ribonucleotides, allowing a continuous input of these cellular resources. This makes these post‐transcriptional regulators perfect candidates to regulate microbial physiology. This review summarizes the current knowledge and unique properties of ribonucleases in the world of pseudomonads, taking into account genomic context analysis, biological function and strategies to use ribonucleases to improve biotechnological processes.
format	article
author	Patrícia Apura Luis G. Gonçalves Sandra C. Viegas Cecília M. Arraiano
author_facet	Patrícia Apura Luis G. Gonçalves Sandra C. Viegas Cecília M. Arraiano
author_sort	Patrícia Apura
title	The world of ribonucleases from pseudomonads: a short trip through the main features and singularities
title_short	The world of ribonucleases from pseudomonads: a short trip through the main features and singularities
title_full	The world of ribonucleases from pseudomonads: a short trip through the main features and singularities
title_fullStr	The world of ribonucleases from pseudomonads: a short trip through the main features and singularities
title_full_unstemmed	The world of ribonucleases from pseudomonads: a short trip through the main features and singularities
title_sort	world of ribonucleases from pseudomonads: a short trip through the main features and singularities
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/ea6e06f0be934d16b320021dbf276563
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The world of ribonucleases from pseudomonads: a short trip through the main features and singularities

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