MR-compatible optical microscope for in-situ dual-mode MR-optical microscopy.

We present the development of a dual-mode imaging platform that combines optical microscopy with magnetic resonance microscopy. Our microscope is designed to operate inside a 9.4T small animal scanner with the option to use a 72mm bore animal RF coil or different integrated linear micro coils. With...

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Autores principales:	Matthias C Wapler, Frederik Testud, Patrick Hucker, Jochen Leupold, Dominik von Elverfeldt, Maxim Zaitsev, Ulrike Wallrabe
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ea7211c335ec404daf76e7eb60c21c7e
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spelling	oai:doaj.org-article:ea7211c335ec404daf76e7eb60c21c7e2021-12-02T20:11:23ZMR-compatible optical microscope for in-situ dual-mode MR-optical microscopy.1932-620310.1371/journal.pone.0250903https://doaj.org/article/ea7211c335ec404daf76e7eb60c21c7e2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0250903https://doaj.org/toc/1932-6203We present the development of a dual-mode imaging platform that combines optical microscopy with magnetic resonance microscopy. Our microscope is designed to operate inside a 9.4T small animal scanner with the option to use a 72mm bore animal RF coil or different integrated linear micro coils. With a design that minimizes the magnetic distortions near the sample, we achieved a field inhomogeneity of 19 ppb RMS. We further integrated a waveguide in the optical layout for the electromagnetic shielding of the camera, which minimizes the noise increase in the MR and optical images below practical relevance. The optical layout uses an adaptive lens for focusing, 2 × 2 modular combinations of objectives with 0.6mm to 2.3mm field of view and 4 configurable RGBW illumination channels and achieves a plano-apochromatic optical aberration correction with 0.6μm to 2.3μm resolution. We present the design, implementation and characterization of the prototype including the general optical and MR-compatible design strategies, a knife-edge optical characterization and different concurrent imaging demonstrations.Matthias C WaplerFrederik TestudPatrick HuckerJochen LeupoldDominik von ElverfeldtMaxim ZaitsevUlrike WallrabePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0250903 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matthias C Wapler Frederik Testud Patrick Hucker Jochen Leupold Dominik von Elverfeldt Maxim Zaitsev Ulrike Wallrabe MR-compatible optical microscope for in-situ dual-mode MR-optical microscopy.
description	We present the development of a dual-mode imaging platform that combines optical microscopy with magnetic resonance microscopy. Our microscope is designed to operate inside a 9.4T small animal scanner with the option to use a 72mm bore animal RF coil or different integrated linear micro coils. With a design that minimizes the magnetic distortions near the sample, we achieved a field inhomogeneity of 19 ppb RMS. We further integrated a waveguide in the optical layout for the electromagnetic shielding of the camera, which minimizes the noise increase in the MR and optical images below practical relevance. The optical layout uses an adaptive lens for focusing, 2 × 2 modular combinations of objectives with 0.6mm to 2.3mm field of view and 4 configurable RGBW illumination channels and achieves a plano-apochromatic optical aberration correction with 0.6μm to 2.3μm resolution. We present the design, implementation and characterization of the prototype including the general optical and MR-compatible design strategies, a knife-edge optical characterization and different concurrent imaging demonstrations.
format	article
author	Matthias C Wapler Frederik Testud Patrick Hucker Jochen Leupold Dominik von Elverfeldt Maxim Zaitsev Ulrike Wallrabe
author_facet	Matthias C Wapler Frederik Testud Patrick Hucker Jochen Leupold Dominik von Elverfeldt Maxim Zaitsev Ulrike Wallrabe
author_sort	Matthias C Wapler
title	MR-compatible optical microscope for in-situ dual-mode MR-optical microscopy.
title_short	MR-compatible optical microscope for in-situ dual-mode MR-optical microscopy.
title_full	MR-compatible optical microscope for in-situ dual-mode MR-optical microscopy.
title_fullStr	MR-compatible optical microscope for in-situ dual-mode MR-optical microscopy.
title_full_unstemmed	MR-compatible optical microscope for in-situ dual-mode MR-optical microscopy.
title_sort	mr-compatible optical microscope for in-situ dual-mode mr-optical microscopy.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ea7211c335ec404daf76e7eb60c21c7e
work_keys_str_mv	AT matthiascwapler mrcompatibleopticalmicroscopeforinsitudualmodemropticalmicroscopy AT frederiktestud mrcompatibleopticalmicroscopeforinsitudualmodemropticalmicroscopy AT patrickhucker mrcompatibleopticalmicroscopeforinsitudualmodemropticalmicroscopy AT jochenleupold mrcompatibleopticalmicroscopeforinsitudualmodemropticalmicroscopy AT dominikvonelverfeldt mrcompatibleopticalmicroscopeforinsitudualmodemropticalmicroscopy AT maximzaitsev mrcompatibleopticalmicroscopeforinsitudualmodemropticalmicroscopy AT ulrikewallrabe mrcompatibleopticalmicroscopeforinsitudualmodemropticalmicroscopy
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MR-compatible optical microscope for in-situ dual-mode MR-optical microscopy.

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