Cytotoxicity analysis of three Bacillus thuringiensis subsp. israelensis δ-endotoxins towards insect and mammalian cells.

Three members of the δ-endotoxin group of toxins expressed by Bacillus thuringiensis subsp. israelensis, Cyt2Ba, Cry4Aa and Cry11A, were individually expressed in recombinant acrystalliferous B. thuringiensis strains for in vitro evaluation of their toxic activities against insect and mammalian cell...

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Autores principales:	Roberto Franco Teixeira Corrêa, Daniel Mendes Pereira Ardisson-Araújo, Rose Gomes Monnerat, Bergmann Morais Ribeiro
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ea73ad0bbf2e47509df910fa210f406e
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spelling	oai:doaj.org-article:ea73ad0bbf2e47509df910fa210f406e2021-11-18T07:04:27ZCytotoxicity analysis of three Bacillus thuringiensis subsp. israelensis δ-endotoxins towards insect and mammalian cells.1932-620310.1371/journal.pone.0046121https://doaj.org/article/ea73ad0bbf2e47509df910fa210f406e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23029407/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Three members of the δ-endotoxin group of toxins expressed by Bacillus thuringiensis subsp. israelensis, Cyt2Ba, Cry4Aa and Cry11A, were individually expressed in recombinant acrystalliferous B. thuringiensis strains for in vitro evaluation of their toxic activities against insect and mammalian cell lines. Both Cry4Aa and Cry11A toxins, activated with either trypsin or Spodoptera frugiperda gastric juice (GJ), resulted in different cleavage patterns for the activated toxins as seen by SDS-PAGE. The GJ-processed proteins were not cytotoxic to insect cell cultures. On the other hand, the combination of the trypsin-activated Cry4Aa and Cry11A toxins yielded the highest levels of cytotoxicity to all insect cells tested. The combination of activated Cyt2Ba and Cry11A also showed higher toxic activity than that of toxins activated individually. When activated Cry4Aa, Cry11A and Cyt2Ba were used simultaneously in the same assay a decrease in toxic activity was observed in all insect cells tested. No toxic effect was observed for the trypsin-activated Cry toxins in mammalian cells, but activated Cyt2Ba was toxic to human breast cancer cells (MCF-7) when tested at 20 µg/mL.Roberto Franco Teixeira CorrêaDaniel Mendes Pereira Ardisson-AraújoRose Gomes MonneratBergmann Morais RibeiroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e46121 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Roberto Franco Teixeira Corrêa Daniel Mendes Pereira Ardisson-Araújo Rose Gomes Monnerat Bergmann Morais Ribeiro Cytotoxicity analysis of three Bacillus thuringiensis subsp. israelensis δ-endotoxins towards insect and mammalian cells.
description	Three members of the δ-endotoxin group of toxins expressed by Bacillus thuringiensis subsp. israelensis, Cyt2Ba, Cry4Aa and Cry11A, were individually expressed in recombinant acrystalliferous B. thuringiensis strains for in vitro evaluation of their toxic activities against insect and mammalian cell lines. Both Cry4Aa and Cry11A toxins, activated with either trypsin or Spodoptera frugiperda gastric juice (GJ), resulted in different cleavage patterns for the activated toxins as seen by SDS-PAGE. The GJ-processed proteins were not cytotoxic to insect cell cultures. On the other hand, the combination of the trypsin-activated Cry4Aa and Cry11A toxins yielded the highest levels of cytotoxicity to all insect cells tested. The combination of activated Cyt2Ba and Cry11A also showed higher toxic activity than that of toxins activated individually. When activated Cry4Aa, Cry11A and Cyt2Ba were used simultaneously in the same assay a decrease in toxic activity was observed in all insect cells tested. No toxic effect was observed for the trypsin-activated Cry toxins in mammalian cells, but activated Cyt2Ba was toxic to human breast cancer cells (MCF-7) when tested at 20 µg/mL.
format	article
author	Roberto Franco Teixeira Corrêa Daniel Mendes Pereira Ardisson-Araújo Rose Gomes Monnerat Bergmann Morais Ribeiro
author_facet	Roberto Franco Teixeira Corrêa Daniel Mendes Pereira Ardisson-Araújo Rose Gomes Monnerat Bergmann Morais Ribeiro
author_sort	Roberto Franco Teixeira Corrêa
title	Cytotoxicity analysis of three Bacillus thuringiensis subsp. israelensis δ-endotoxins towards insect and mammalian cells.
title_short	Cytotoxicity analysis of three Bacillus thuringiensis subsp. israelensis δ-endotoxins towards insect and mammalian cells.
title_full	Cytotoxicity analysis of three Bacillus thuringiensis subsp. israelensis δ-endotoxins towards insect and mammalian cells.
title_fullStr	Cytotoxicity analysis of three Bacillus thuringiensis subsp. israelensis δ-endotoxins towards insect and mammalian cells.
title_full_unstemmed	Cytotoxicity analysis of three Bacillus thuringiensis subsp. israelensis δ-endotoxins towards insect and mammalian cells.
title_sort	cytotoxicity analysis of three bacillus thuringiensis subsp. israelensis δ-endotoxins towards insect and mammalian cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/ea73ad0bbf2e47509df910fa210f406e
work_keys_str_mv	AT robertofrancoteixeiracorrea cytotoxicityanalysisofthreebacillusthuringiensissubspisraelensisdendotoxinstowardsinsectandmammaliancells AT danielmendespereiraardissonaraujo cytotoxicityanalysisofthreebacillusthuringiensissubspisraelensisdendotoxinstowardsinsectandmammaliancells AT rosegomesmonnerat cytotoxicityanalysisofthreebacillusthuringiensissubspisraelensisdendotoxinstowardsinsectandmammaliancells AT bergmannmoraisribeiro cytotoxicityanalysisofthreebacillusthuringiensissubspisraelensisdendotoxinstowardsinsectandmammaliancells
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Cytotoxicity analysis of three Bacillus thuringiensis subsp. israelensis δ-endotoxins towards insect and mammalian cells.

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