Anxiety and Cognition in Cre- Collagen Type II Sirt1 K/O Male Mice

IntroductionUsing transgenic collagen type II-specific Sirt1 knockout (CKO) mice we studied the role of Sirt1 in nutritional induced catch up growth (CUG) and we found that these mice have a less organized growth plate and reduced efficiency of CUG. In addition, we noted that they weigh more than co...

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Autores principales:	Biana Shtaif, Shay Henry Hornfeld, Michal Yackobovitch-Gavan, Moshe Phillip, Galia Gat-Yablonski
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	SIRT1 anxiety cognition collagen type II osteocalcin Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/ea76f0e04a9e409d84af1482490cff1c
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spelling	oai:doaj.org-article:ea76f0e04a9e409d84af1482490cff1c2021-11-19T15:08:45ZAnxiety and Cognition in Cre- Collagen Type II Sirt1 K/O Male Mice1664-239210.3389/fendo.2021.756909https://doaj.org/article/ea76f0e04a9e409d84af1482490cff1c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fendo.2021.756909/fullhttps://doaj.org/toc/1664-2392IntroductionUsing transgenic collagen type II-specific Sirt1 knockout (CKO) mice we studied the role of Sirt1 in nutritional induced catch up growth (CUG) and we found that these mice have a less organized growth plate and reduced efficiency of CUG. In addition, we noted that they weigh more than control (CTL) mice. Studying the reason for the increased weigh, we found differences in activity and brain function.MethodsSeveral tests for behavior and activity were used: open field; elevated plus maze, Morris water maze, and home cage running wheels. The level of Glu- osteocalcin, known to connect bone and brain function, was measured by Elisa; brain Sirt1 was analyzed by western blot.ResultsWe found that CKO mice had increased anxiety, with less spatial memory, learning capabilities and reduced activity in their home cages. No significant differences were found between CKO and CTL mice in Glu- osteocalcin levels; nor in the level of brain SIRT1.Discussion/ConclusionUsing transgenic collagen type II-specific Sirt1 knockout (CKO) mice we found a close connection between linear growth and brain function. Using a collagen type II derived system we affected a central regulatory mechanism leading to hypo activity, increased anxiety, and slower learning, without affecting circadian period. As children with idiopathic short stature are more likely to have lower IQ, with substantial deficits in working memory than healthy controls, the results of the current study suggest that SIRT1 may be the underlying factor connecting growth and brain function.Biana ShtaifBiana ShtaifShay Henry HornfeldMichal Yackobovitch-GavanMichal Yackobovitch-GavanMoshe PhillipMoshe PhillipMoshe PhillipGalia Gat-YablonskiGalia Gat-YablonskiGalia Gat-YablonskiFrontiers Media S.A.articleSIRT1anxietycognitioncollagen type IIosteocalcinDiseases of the endocrine glands. Clinical endocrinologyRC648-665ENFrontiers in Endocrinology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SIRT1 anxiety cognition collagen type II osteocalcin Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	SIRT1 anxiety cognition collagen type II osteocalcin Diseases of the endocrine glands. Clinical endocrinology RC648-665 Biana Shtaif Biana Shtaif Shay Henry Hornfeld Michal Yackobovitch-Gavan Michal Yackobovitch-Gavan Moshe Phillip Moshe Phillip Moshe Phillip Galia Gat-Yablonski Galia Gat-Yablonski Galia Gat-Yablonski Anxiety and Cognition in Cre- Collagen Type II Sirt1 K/O Male Mice
description	IntroductionUsing transgenic collagen type II-specific Sirt1 knockout (CKO) mice we studied the role of Sirt1 in nutritional induced catch up growth (CUG) and we found that these mice have a less organized growth plate and reduced efficiency of CUG. In addition, we noted that they weigh more than control (CTL) mice. Studying the reason for the increased weigh, we found differences in activity and brain function.MethodsSeveral tests for behavior and activity were used: open field; elevated plus maze, Morris water maze, and home cage running wheels. The level of Glu- osteocalcin, known to connect bone and brain function, was measured by Elisa; brain Sirt1 was analyzed by western blot.ResultsWe found that CKO mice had increased anxiety, with less spatial memory, learning capabilities and reduced activity in their home cages. No significant differences were found between CKO and CTL mice in Glu- osteocalcin levels; nor in the level of brain SIRT1.Discussion/ConclusionUsing transgenic collagen type II-specific Sirt1 knockout (CKO) mice we found a close connection between linear growth and brain function. Using a collagen type II derived system we affected a central regulatory mechanism leading to hypo activity, increased anxiety, and slower learning, without affecting circadian period. As children with idiopathic short stature are more likely to have lower IQ, with substantial deficits in working memory than healthy controls, the results of the current study suggest that SIRT1 may be the underlying factor connecting growth and brain function.
format	article
author	Biana Shtaif Biana Shtaif Shay Henry Hornfeld Michal Yackobovitch-Gavan Michal Yackobovitch-Gavan Moshe Phillip Moshe Phillip Moshe Phillip Galia Gat-Yablonski Galia Gat-Yablonski Galia Gat-Yablonski
author_facet	Biana Shtaif Biana Shtaif Shay Henry Hornfeld Michal Yackobovitch-Gavan Michal Yackobovitch-Gavan Moshe Phillip Moshe Phillip Moshe Phillip Galia Gat-Yablonski Galia Gat-Yablonski Galia Gat-Yablonski
author_sort	Biana Shtaif
title	Anxiety and Cognition in Cre- Collagen Type II Sirt1 K/O Male Mice
title_short	Anxiety and Cognition in Cre- Collagen Type II Sirt1 K/O Male Mice
title_full	Anxiety and Cognition in Cre- Collagen Type II Sirt1 K/O Male Mice
title_fullStr	Anxiety and Cognition in Cre- Collagen Type II Sirt1 K/O Male Mice
title_full_unstemmed	Anxiety and Cognition in Cre- Collagen Type II Sirt1 K/O Male Mice
title_sort	anxiety and cognition in cre- collagen type ii sirt1 k/o male mice
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ea76f0e04a9e409d84af1482490cff1c
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Anxiety and Cognition in Cre- Collagen Type II Sirt1 K/O Male Mice

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