Acute and subacute toxicity studies of a new herbal formula induced apoptosis in the highly metastatic MDA-MB-231 cells

Breast cancer is one of the major causes of cancer deaths worldwide, and survival rate remains low even with the advancement of screening and treatment methods. Polyherbal medicines have been widely used for cancer treatment. CBBh and SNH are two new polyherbal formulations that showed anticancer ef...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Saad M. Aljari, Nael Abutaha, Lamya Ahmed Al-Keridis, Amin A. Al-Doaiss, Fahd AL-Mekhlafi, Muhammad A. Wadaan
Formato: article
Lenguaje:EN
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://doaj.org/article/ea787456991a4f6ab4c7002c84f90b03
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:ea787456991a4f6ab4c7002c84f90b03
record_format dspace
spelling oai:doaj.org-article:ea787456991a4f6ab4c7002c84f90b032021-11-18T04:44:21ZAcute and subacute toxicity studies of a new herbal formula induced apoptosis in the highly metastatic MDA-MB-231 cells1018-364710.1016/j.jksus.2021.101646https://doaj.org/article/ea787456991a4f6ab4c7002c84f90b032021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721003086https://doaj.org/toc/1018-3647Breast cancer is one of the major causes of cancer deaths worldwide, and survival rate remains low even with the advancement of screening and treatment methods. Polyherbal medicines have been widely used for cancer treatment. CBBh and SNH are two new polyherbal formulations that showed anticancer effects.MDA-MB-231 and MCF-7 cells were used for in vitro investigation. After treatments with CBBh and SNH, the proliferation and apoptosis of cells were detected by MTT assay, fluorescent microscopy, and Muse cell analyzer. The acute and subacute toxicities were studied following Organization for Economic Cooperation and Development (OECD) guidelines. Gas chromatography-mass spectrometry (GC–MS) was applied for analysis of chemical constituents of the extract.CBBh extract inhibited proliferation of MCF-7 (IC50: 109.7 μg/mL) and MDA-MB-231 (IC50: 71.1 μg/mL) cancer cells, whereas the IC50 value for SNH was higher than 400 μg/mL against both cancer cell lines. The percentages of apoptotic cells following treatment with 200 and 300 μg/mL for 24 h were 38.9 ± 2.8% and 76.2 ± 2.1% MDA-MB231 cells respectively, versus 0.07% and 5.21% respectively in vehicle-treated cells. No acute or subacute oral toxicity was observed. CBBh extract was tolerated in concentrations up to 2000 mg/kg. Acute and subacute histopathological examination of the liver and kidneys revealed no abnormalities in their architecture.The new polyherbal formula CBBh could suppress breast cancer through apoptosis. Therefore, our findings could provide a novel herbal formula for the treatment of breast cancer.Saad M. AljariNael AbutahaLamya Ahmed Al-KeridisAmin A. Al-DoaissFahd AL-MekhlafiMuhammad A. WadaanElsevierarticlePolyherbal formulationsAnticancerApoptosisToxicityScience (General)Q1-390ENJournal of King Saud University: Science, Vol 33, Iss 8, Pp 101646- (2021)
institution DOAJ
collection DOAJ
language EN
topic Polyherbal formulations
Anticancer
Apoptosis
Toxicity
Science (General)
Q1-390
spellingShingle Polyherbal formulations
Anticancer
Apoptosis
Toxicity
Science (General)
Q1-390
Saad M. Aljari
Nael Abutaha
Lamya Ahmed Al-Keridis
Amin A. Al-Doaiss
Fahd AL-Mekhlafi
Muhammad A. Wadaan
Acute and subacute toxicity studies of a new herbal formula induced apoptosis in the highly metastatic MDA-MB-231 cells
description Breast cancer is one of the major causes of cancer deaths worldwide, and survival rate remains low even with the advancement of screening and treatment methods. Polyherbal medicines have been widely used for cancer treatment. CBBh and SNH are two new polyherbal formulations that showed anticancer effects.MDA-MB-231 and MCF-7 cells were used for in vitro investigation. After treatments with CBBh and SNH, the proliferation and apoptosis of cells were detected by MTT assay, fluorescent microscopy, and Muse cell analyzer. The acute and subacute toxicities were studied following Organization for Economic Cooperation and Development (OECD) guidelines. Gas chromatography-mass spectrometry (GC–MS) was applied for analysis of chemical constituents of the extract.CBBh extract inhibited proliferation of MCF-7 (IC50: 109.7 μg/mL) and MDA-MB-231 (IC50: 71.1 μg/mL) cancer cells, whereas the IC50 value for SNH was higher than 400 μg/mL against both cancer cell lines. The percentages of apoptotic cells following treatment with 200 and 300 μg/mL for 24 h were 38.9 ± 2.8% and 76.2 ± 2.1% MDA-MB231 cells respectively, versus 0.07% and 5.21% respectively in vehicle-treated cells. No acute or subacute oral toxicity was observed. CBBh extract was tolerated in concentrations up to 2000 mg/kg. Acute and subacute histopathological examination of the liver and kidneys revealed no abnormalities in their architecture.The new polyherbal formula CBBh could suppress breast cancer through apoptosis. Therefore, our findings could provide a novel herbal formula for the treatment of breast cancer.
format article
author Saad M. Aljari
Nael Abutaha
Lamya Ahmed Al-Keridis
Amin A. Al-Doaiss
Fahd AL-Mekhlafi
Muhammad A. Wadaan
author_facet Saad M. Aljari
Nael Abutaha
Lamya Ahmed Al-Keridis
Amin A. Al-Doaiss
Fahd AL-Mekhlafi
Muhammad A. Wadaan
author_sort Saad M. Aljari
title Acute and subacute toxicity studies of a new herbal formula induced apoptosis in the highly metastatic MDA-MB-231 cells
title_short Acute and subacute toxicity studies of a new herbal formula induced apoptosis in the highly metastatic MDA-MB-231 cells
title_full Acute and subacute toxicity studies of a new herbal formula induced apoptosis in the highly metastatic MDA-MB-231 cells
title_fullStr Acute and subacute toxicity studies of a new herbal formula induced apoptosis in the highly metastatic MDA-MB-231 cells
title_full_unstemmed Acute and subacute toxicity studies of a new herbal formula induced apoptosis in the highly metastatic MDA-MB-231 cells
title_sort acute and subacute toxicity studies of a new herbal formula induced apoptosis in the highly metastatic mda-mb-231 cells
publisher Elsevier
publishDate 2021
url https://doaj.org/article/ea787456991a4f6ab4c7002c84f90b03
work_keys_str_mv AT saadmaljari acuteandsubacutetoxicitystudiesofanewherbalformulainducedapoptosisinthehighlymetastaticmdamb231cells
AT naelabutaha acuteandsubacutetoxicitystudiesofanewherbalformulainducedapoptosisinthehighlymetastaticmdamb231cells
AT lamyaahmedalkeridis acuteandsubacutetoxicitystudiesofanewherbalformulainducedapoptosisinthehighlymetastaticmdamb231cells
AT aminaaldoaiss acuteandsubacutetoxicitystudiesofanewherbalformulainducedapoptosisinthehighlymetastaticmdamb231cells
AT fahdalmekhlafi acuteandsubacutetoxicitystudiesofanewherbalformulainducedapoptosisinthehighlymetastaticmdamb231cells
AT muhammadawadaan acuteandsubacutetoxicitystudiesofanewherbalformulainducedapoptosisinthehighlymetastaticmdamb231cells
_version_ 1718425135395897344