Study of microbiocenosis of canine dental biofilms

Abstract Dental biofilm is a complex microbial community influenced by many exogenous and endogenous factors. Despite long-term studies, its bacterial composition is still not clearly understood. While most of the research on dental biofilms was conducted in humans, much less information is availabl...

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Autores principales:	Jana Kačírová, Aladár Maďari, Rastislav Mucha, Lívia K. Fecskeová, Izabela Mujakic, Michal Koblížek, Radomíra Nemcová, Marián Maďar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ea7e649e30b24446a7fd53056a456a29
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spelling	oai:doaj.org-article:ea7e649e30b24446a7fd53056a456a292021-12-02T18:01:40ZStudy of microbiocenosis of canine dental biofilms10.1038/s41598-021-99342-52045-2322https://doaj.org/article/ea7e649e30b24446a7fd53056a456a292021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99342-5https://doaj.org/toc/2045-2322Abstract Dental biofilm is a complex microbial community influenced by many exogenous and endogenous factors. Despite long-term studies, its bacterial composition is still not clearly understood. While most of the research on dental biofilms was conducted in humans, much less information is available from companion animals. In this study, we analyzed the composition of canine dental biofilms using both standard cultivation on solid media and amplicon sequencing, and compared the two approaches. The 16S rRNA gene sequences were used to define the bacterial community of canine dental biofilm with both, culture-dependent and culture-independent methods. After DNA extraction from each sample, the V3–V4 region of the 16S rRNA gene was amplified and sequenced via Illumina MiSeq platform. Isolated bacteria were identified using universal primers and Sanger sequencing. Representatives of 18 bacterial genera belonging to 5 phyla were isolated from solid media. Amplicon sequencing largely expanded this information identifying in total 284 operational taxonomic units belonging to 10 bacterial phyla. Amplicon sequencing revealed much higher diversity of bacteria in the canine dental biofilms, when compared to standard cultivation approach. In contrast, cultured representatives of several bacterial families were not identified by amplicon sequencing.Jana KačírováAladár MaďariRastislav MuchaLívia K. FecskeováIzabela MujakicMichal KoblížekRadomíra NemcováMarián MaďarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jana Kačírová Aladár Maďari Rastislav Mucha Lívia K. Fecskeová Izabela Mujakic Michal Koblížek Radomíra Nemcová Marián Maďar Study of microbiocenosis of canine dental biofilms
description	Abstract Dental biofilm is a complex microbial community influenced by many exogenous and endogenous factors. Despite long-term studies, its bacterial composition is still not clearly understood. While most of the research on dental biofilms was conducted in humans, much less information is available from companion animals. In this study, we analyzed the composition of canine dental biofilms using both standard cultivation on solid media and amplicon sequencing, and compared the two approaches. The 16S rRNA gene sequences were used to define the bacterial community of canine dental biofilm with both, culture-dependent and culture-independent methods. After DNA extraction from each sample, the V3–V4 region of the 16S rRNA gene was amplified and sequenced via Illumina MiSeq platform. Isolated bacteria were identified using universal primers and Sanger sequencing. Representatives of 18 bacterial genera belonging to 5 phyla were isolated from solid media. Amplicon sequencing largely expanded this information identifying in total 284 operational taxonomic units belonging to 10 bacterial phyla. Amplicon sequencing revealed much higher diversity of bacteria in the canine dental biofilms, when compared to standard cultivation approach. In contrast, cultured representatives of several bacterial families were not identified by amplicon sequencing.
format	article
author	Jana Kačírová Aladár Maďari Rastislav Mucha Lívia K. Fecskeová Izabela Mujakic Michal Koblížek Radomíra Nemcová Marián Maďar
author_facet	Jana Kačírová Aladár Maďari Rastislav Mucha Lívia K. Fecskeová Izabela Mujakic Michal Koblížek Radomíra Nemcová Marián Maďar
author_sort	Jana Kačírová
title	Study of microbiocenosis of canine dental biofilms
title_short	Study of microbiocenosis of canine dental biofilms
title_full	Study of microbiocenosis of canine dental biofilms
title_fullStr	Study of microbiocenosis of canine dental biofilms
title_full_unstemmed	Study of microbiocenosis of canine dental biofilms
title_sort	study of microbiocenosis of canine dental biofilms
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ea7e649e30b24446a7fd53056a456a29
work_keys_str_mv	AT janakacirova studyofmicrobiocenosisofcaninedentalbiofilms AT aladarmadari studyofmicrobiocenosisofcaninedentalbiofilms AT rastislavmucha studyofmicrobiocenosisofcaninedentalbiofilms AT liviakfecskeova studyofmicrobiocenosisofcaninedentalbiofilms AT izabelamujakic studyofmicrobiocenosisofcaninedentalbiofilms AT michalkoblizek studyofmicrobiocenosisofcaninedentalbiofilms AT radomiranemcova studyofmicrobiocenosisofcaninedentalbiofilms AT marianmadar studyofmicrobiocenosisofcaninedentalbiofilms
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Study of microbiocenosis of canine dental biofilms

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