Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway

Zhengming Li1,*, Pijian Yang2,*, Yuzhen Liang1,*, Ning Xia2,*, Yingrong Li1, Hongye Su1, Hailin Pan1 1Department of Endocrinology and Metabolism, Second Affiliated Hospital of Guangxi Medical University, Nanning, People’s Republic of China; 2Department of Endocrinology and Metabolism, The...

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Autores principales:	Li Z, Yang P, Liang Y, Xia N, Li Y, Su H, Pan H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	liraglutide obesity lipolysis adenylate cyclase 3 hormone-sensitive lipase Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/ea800619b7954806bd9de5ccd9934180
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spelling	oai:doaj.org-article:ea800619b7954806bd9de5ccd99341802021-12-02T04:10:04ZEffects of liraglutide on lipolysis and the AC3/PKA/HSL pathway1178-7007https://doaj.org/article/ea800619b7954806bd9de5ccd99341802019-09-01T00:00:00Zhttps://www.dovepress.com/effects-of-liraglutide-on-lipolysis-and-the-ac3pkahsl-pathway-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Zhengming Li1,, Pijian Yang2,, Yuzhen Liang1,, Ning Xia2,, Yingrong Li1, Hongye Su1, Hailin Pan1 1Department of Endocrinology and Metabolism, Second Affiliated Hospital of Guangxi Medical University, Nanning, People’s Republic of China; 2Department of Endocrinology and Metabolism, The First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Nanning, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yuzhen LiangDepartment of Endocrinology and Metabolism, Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, People’s Republic of ChinaEmail liangyuzhen26@163.comNing XiaDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Nanning 530021, People’s Republic of ChinaEmail xianinggxmu@163.comBackground: Liraglutide reduces blood glucose, body weight and blood lipid levels. Hormone-sensitive lipase (HSL) is a key enzyme in lipolysis. Evidence from our and other studies have demonstrated that adenylate cyclase 3 (AC3) is associated with obesity and can be upregulated by liraglutide in obese mice. In the present study, we investigated whether hepatic HSL activity is regulated by liraglutide and characterized the effect of liraglutide in the AC3/protein kinase A (PKA)/HSL signalling pathway.Methods: Obese mice or their lean littermates were treated with liraglutide or saline for 8 weeks. Serum was collected for the measurement of insulin and lipids. We investigated hepatic AC3, HSL and phosphorylated HSL Ser-660 (p-HSL(S660)) protein expression levels andAC3 and HSL mRNA expression levels and cyclic adenosine monophosphate (cAMP), PKA activity in liver tissue.Results: Liraglutide treatment decreased triglycerides (TGs) and free fatty acids (FFAs), increased glycerol, and upregulated hepatic AC3 and p-HSL(s660) levels and cAMP and PKA activities.Conclusion: The results suggest that liraglutide can upregulates AC3/PKA/HSL pathway and may promotes lipolysis.Keywords: liraglutide, obesity, lipolysis, adenylate cyclase 3, hormone-sensitive lipaseLi ZYang PLiang YXia NLi YSu HPan HDove Medical Pressarticleliraglutideobesitylipolysisadenylate cyclase 3hormone-sensitive lipaseSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 1697-1703 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	liraglutide obesity lipolysis adenylate cyclase 3 hormone-sensitive lipase Specialties of internal medicine RC581-951
spellingShingle	liraglutide obesity lipolysis adenylate cyclase 3 hormone-sensitive lipase Specialties of internal medicine RC581-951 Li Z Yang P Liang Y Xia N Li Y Su H Pan H Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway
description	Zhengming Li1,, Pijian Yang2,, Yuzhen Liang1,, Ning Xia2,, Yingrong Li1, Hongye Su1, Hailin Pan1 1Department of Endocrinology and Metabolism, Second Affiliated Hospital of Guangxi Medical University, Nanning, People’s Republic of China; 2Department of Endocrinology and Metabolism, The First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Nanning, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yuzhen LiangDepartment of Endocrinology and Metabolism, Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, People’s Republic of ChinaEmail liangyuzhen26@163.comNing XiaDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Nanning 530021, People’s Republic of ChinaEmail xianinggxmu@163.comBackground: Liraglutide reduces blood glucose, body weight and blood lipid levels. Hormone-sensitive lipase (HSL) is a key enzyme in lipolysis. Evidence from our and other studies have demonstrated that adenylate cyclase 3 (AC3) is associated with obesity and can be upregulated by liraglutide in obese mice. In the present study, we investigated whether hepatic HSL activity is regulated by liraglutide and characterized the effect of liraglutide in the AC3/protein kinase A (PKA)/HSL signalling pathway.Methods: Obese mice or their lean littermates were treated with liraglutide or saline for 8 weeks. Serum was collected for the measurement of insulin and lipids. We investigated hepatic AC3, HSL and phosphorylated HSL Ser-660 (p-HSL(S660)) protein expression levels andAC3 and HSL mRNA expression levels and cyclic adenosine monophosphate (cAMP), PKA activity in liver tissue.Results: Liraglutide treatment decreased triglycerides (TGs) and free fatty acids (FFAs), increased glycerol, and upregulated hepatic AC3 and p-HSL(s660) levels and cAMP and PKA activities.Conclusion: The results suggest that liraglutide can upregulates AC3/PKA/HSL pathway and may promotes lipolysis.Keywords: liraglutide, obesity, lipolysis, adenylate cyclase 3, hormone-sensitive lipase
format	article
author	Li Z Yang P Liang Y Xia N Li Y Su H Pan H
author_facet	Li Z Yang P Liang Y Xia N Li Y Su H Pan H
author_sort	Li Z
title	Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway
title_short	Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway
title_full	Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway
title_fullStr	Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway
title_full_unstemmed	Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway
title_sort	effects of liraglutide on lipolysis and the ac3/pka/hsl pathway
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/ea800619b7954806bd9de5ccd9934180
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Effects of liraglutide on lipolysis and the AC3/PKA/HSL pathway

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