Expression of the miR-302/367 microRNA cluster is regulated by a conserved long non-coding host-gene

Abstract MicroRNAs are important regulators of cellular functions. MiR-302/367 is a polycistronic miRNA cluster that can induce and maintain pluripotency. Here we investigate the transcriptional control and the processing of the miR-302 host-gene in mice. Our results indicate that the mmu-miR-302 ho...

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Autores principales:	Karim Rahimi, Annette Christine Füchtbauer, Fardin Fathi, Seyed Javad Mowla, Ernst-Martin Füchtbauer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ea8678ee12cb4e4f9c63b22ecb7f8bb3
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spelling	oai:doaj.org-article:ea8678ee12cb4e4f9c63b22ecb7f8bb32021-12-02T15:49:45ZExpression of the miR-302/367 microRNA cluster is regulated by a conserved long non-coding host-gene10.1038/s41598-021-89080-z2045-2322https://doaj.org/article/ea8678ee12cb4e4f9c63b22ecb7f8bb32021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89080-zhttps://doaj.org/toc/2045-2322Abstract MicroRNAs are important regulators of cellular functions. MiR-302/367 is a polycistronic miRNA cluster that can induce and maintain pluripotency. Here we investigate the transcriptional control and the processing of the miR-302 host-gene in mice. Our results indicate that the mmu-miR-302 host-gene is alternatively spliced, polyadenylated and exported from the nucleus. The regulatory sequences extend at least 2 kb upstream of the transcription start site and contain several conserved binding sites for both transcriptional activators and repressors. The gene structure and regulatory elements are highly conserved between mouse and human. So far, regulating miR-302 expression is the only known function of the miR-302 host-gene. Even though we here only provide one example, regulation of microRNA transcription might be a so far little recognized function of long non-coding RNA genes.Karim RahimiAnnette Christine FüchtbauerFardin FathiSeyed Javad MowlaErnst-Martin FüchtbauerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karim Rahimi Annette Christine Füchtbauer Fardin Fathi Seyed Javad Mowla Ernst-Martin Füchtbauer Expression of the miR-302/367 microRNA cluster is regulated by a conserved long non-coding host-gene
description	Abstract MicroRNAs are important regulators of cellular functions. MiR-302/367 is a polycistronic miRNA cluster that can induce and maintain pluripotency. Here we investigate the transcriptional control and the processing of the miR-302 host-gene in mice. Our results indicate that the mmu-miR-302 host-gene is alternatively spliced, polyadenylated and exported from the nucleus. The regulatory sequences extend at least 2 kb upstream of the transcription start site and contain several conserved binding sites for both transcriptional activators and repressors. The gene structure and regulatory elements are highly conserved between mouse and human. So far, regulating miR-302 expression is the only known function of the miR-302 host-gene. Even though we here only provide one example, regulation of microRNA transcription might be a so far little recognized function of long non-coding RNA genes.
format	article
author	Karim Rahimi Annette Christine Füchtbauer Fardin Fathi Seyed Javad Mowla Ernst-Martin Füchtbauer
author_facet	Karim Rahimi Annette Christine Füchtbauer Fardin Fathi Seyed Javad Mowla Ernst-Martin Füchtbauer
author_sort	Karim Rahimi
title	Expression of the miR-302/367 microRNA cluster is regulated by a conserved long non-coding host-gene
title_short	Expression of the miR-302/367 microRNA cluster is regulated by a conserved long non-coding host-gene
title_full	Expression of the miR-302/367 microRNA cluster is regulated by a conserved long non-coding host-gene
title_fullStr	Expression of the miR-302/367 microRNA cluster is regulated by a conserved long non-coding host-gene
title_full_unstemmed	Expression of the miR-302/367 microRNA cluster is regulated by a conserved long non-coding host-gene
title_sort	expression of the mir-302/367 microrna cluster is regulated by a conserved long non-coding host-gene
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ea8678ee12cb4e4f9c63b22ecb7f8bb3
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Expression of the miR-302/367 microRNA cluster is regulated by a conserved long non-coding host-gene

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