Administration of troxerutin and cerebroprotein hydrolysate injection alleviates cerebral ischemia/reperfusion injury by down-regulating caspase molecules

Rubo Sui, Lie Zang, Yanjuan BaiDepartment of Neurology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, Liaoning, People’s Republic of ChinaCorrespondence: Rubo SuiDepartment of Neurology, The First Affiliated Hospital of Jinzhou Medical University, 2 Renmin Ro...

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Autores principales:	Sui R, Zang L, Bai Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	ischemia/reperfusion injury troxerutin and cerebroprotein hydrolysate injections apoptosis caspase Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/ea9454ef9aa54d30a25df9e6728bb5b0
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spelling	oai:doaj.org-article:ea9454ef9aa54d30a25df9e6728bb5b02021-12-02T05:41:53ZAdministration of troxerutin and cerebroprotein hydrolysate injection alleviates cerebral ischemia/reperfusion injury by down-regulating caspase molecules1178-2021https://doaj.org/article/ea9454ef9aa54d30a25df9e6728bb5b02019-08-01T00:00:00Zhttps://www.dovepress.com/administration-of-troxerutin-and-cerebroprotein-hydrolysate-injection--peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Rubo Sui, Lie Zang, Yanjuan BaiDepartment of Neurology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, Liaoning, People’s Republic of ChinaCorrespondence: Rubo SuiDepartment of Neurology, The First Affiliated Hospital of Jinzhou Medical University, 2 Renmin Road, Jinzhou 121000, Liaoning, People’s Republic of ChinaEmail yaanddoy@sina.comBackground: Cerebral ischemia/reperfusion injury (I/R injury) is an important pathological process for nervous system. The I/R injury usually causes cerebral hypoxia, infarct or stroke. This study aimed to evaluate effects of troxerutin and cerebroprotein hydrolysate injection (TC) on I/R injury in rat models.Methods: Middle-cerebral artery occlusion/reperfusion (MCAO/R) rat models were established. Rats were divided into normal control (NC), MCAO/R rat model (injecting saline) and MCAO/R rats administrating with TC group (injecting with TC at concentration of 2 mL/100 g body weight). Neurological scores were evaluated with Garcia scale. Magnetic resonance imaging (MRI) was employed to observe infarct area, contralateral area and apparent diffusion coefficient (ADC) values. Cerebral infarct size was examined and visualized by staining with 2,3,5-triphenyltetrazolium chloride (TTC). Western blotting assay was used to determine caspase-1, caspase-3 and caspase-8 expression.Results: The infarct size of mice in MCAO/R+TC group was smaller significantly compared to that in MCAO/R group (p<0.05). The infarct/contralateral area ratio of T2 and T2 Flair signals in MCAO/R+TC group were lower significantly compared to that in MCAO/R group (p<0.05). ADC values in MCAO/R+TC group were significantly enhanced compared to that in MCAO/R group (p<0.05). The troxerutin and cerebroprotein treatment significantly increased neurological scores compared to that in MCAO/R group (p<0.05). Troxerutin and cerebroprotein treatment significantly decreased expression of caspase-1, caspase-3, caspase-8 compared to that in MCAO/R group (p<0.05).Conclusion: Troxerutin and cerebroprotein administration alleviated cerebral I/R injury by down-regulating caspase molecules.Keywords: ischemia/reperfusion injury, troxerutin and cerebroprotein hydrolysate injections, apoptosis, caspaseSui RZang LBai YDove Medical Pressarticleischemia/reperfusion injurytroxerutin and cerebroprotein hydrolysate injectionsapoptosiscaspaseNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 15, Pp 2345-2352 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	ischemia/reperfusion injury troxerutin and cerebroprotein hydrolysate injections apoptosis caspase Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	ischemia/reperfusion injury troxerutin and cerebroprotein hydrolysate injections apoptosis caspase Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Sui R Zang L Bai Y Administration of troxerutin and cerebroprotein hydrolysate injection alleviates cerebral ischemia/reperfusion injury by down-regulating caspase molecules
description	Rubo Sui, Lie Zang, Yanjuan BaiDepartment of Neurology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, Liaoning, People’s Republic of ChinaCorrespondence: Rubo SuiDepartment of Neurology, The First Affiliated Hospital of Jinzhou Medical University, 2 Renmin Road, Jinzhou 121000, Liaoning, People’s Republic of ChinaEmail yaanddoy@sina.comBackground: Cerebral ischemia/reperfusion injury (I/R injury) is an important pathological process for nervous system. The I/R injury usually causes cerebral hypoxia, infarct or stroke. This study aimed to evaluate effects of troxerutin and cerebroprotein hydrolysate injection (TC) on I/R injury in rat models.Methods: Middle-cerebral artery occlusion/reperfusion (MCAO/R) rat models were established. Rats were divided into normal control (NC), MCAO/R rat model (injecting saline) and MCAO/R rats administrating with TC group (injecting with TC at concentration of 2 mL/100 g body weight). Neurological scores were evaluated with Garcia scale. Magnetic resonance imaging (MRI) was employed to observe infarct area, contralateral area and apparent diffusion coefficient (ADC) values. Cerebral infarct size was examined and visualized by staining with 2,3,5-triphenyltetrazolium chloride (TTC). Western blotting assay was used to determine caspase-1, caspase-3 and caspase-8 expression.Results: The infarct size of mice in MCAO/R+TC group was smaller significantly compared to that in MCAO/R group (p<0.05). The infarct/contralateral area ratio of T2 and T2 Flair signals in MCAO/R+TC group were lower significantly compared to that in MCAO/R group (p<0.05). ADC values in MCAO/R+TC group were significantly enhanced compared to that in MCAO/R group (p<0.05). The troxerutin and cerebroprotein treatment significantly increased neurological scores compared to that in MCAO/R group (p<0.05). Troxerutin and cerebroprotein treatment significantly decreased expression of caspase-1, caspase-3, caspase-8 compared to that in MCAO/R group (p<0.05).Conclusion: Troxerutin and cerebroprotein administration alleviated cerebral I/R injury by down-regulating caspase molecules.Keywords: ischemia/reperfusion injury, troxerutin and cerebroprotein hydrolysate injections, apoptosis, caspase
format	article
author	Sui R Zang L Bai Y
author_facet	Sui R Zang L Bai Y
author_sort	Sui R
title	Administration of troxerutin and cerebroprotein hydrolysate injection alleviates cerebral ischemia/reperfusion injury by down-regulating caspase molecules
title_short	Administration of troxerutin and cerebroprotein hydrolysate injection alleviates cerebral ischemia/reperfusion injury by down-regulating caspase molecules
title_full	Administration of troxerutin and cerebroprotein hydrolysate injection alleviates cerebral ischemia/reperfusion injury by down-regulating caspase molecules
title_fullStr	Administration of troxerutin and cerebroprotein hydrolysate injection alleviates cerebral ischemia/reperfusion injury by down-regulating caspase molecules
title_full_unstemmed	Administration of troxerutin and cerebroprotein hydrolysate injection alleviates cerebral ischemia/reperfusion injury by down-regulating caspase molecules
title_sort	administration of troxerutin and cerebroprotein hydrolysate injection alleviates cerebral ischemia/reperfusion injury by down-regulating caspase molecules
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/ea9454ef9aa54d30a25df9e6728bb5b0
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Administration of troxerutin and cerebroprotein hydrolysate injection alleviates cerebral ischemia/reperfusion injury by down-regulating caspase molecules

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