A low-cost and open-source protocol to produce key enzymes for molecular detection assays
Summary: Here, we describe a detailed step-by-step protocol for the expression, purification, quantification, and activity determination of key enzymes for molecular detection of pathogens. Based on previous reports, we optimized the protocol for LbCas12a, Taq DNA polymerase, M-MLV reverse transcrip...
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Autores principales: | Gabriel Mendoza-Rojas, Vanessa Sarabia-Vega, Ana Sanchez-Castro, Lesia Tello, Luis Cabrera-Sosa, Jose A. Nakamoto, Katherin Peñaranda, Vanessa Adaui, Roberto Alcántara, Pohl Milón |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://doaj.org/article/ea9665530c934376806cf99d74794974 |
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