Two-colour live-cell nanoscale imaging of intracellular targets

The intracellular applications of STED microscopy are limited by the availability of dyes. Here the authors develop a two-colour labelling strategy based on SiR and ATTO590 dyes, and apply their strategy to image various subcellular membrane compartments.

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Autores principales:	Francesca Bottanelli, Emil B. Kromann, Edward S. Allgeyer, Roman S. Erdmann, Stephanie Wood Baguley, George Sirinakis, Alanna Schepartz, David Baddeley, Derek K. Toomre, James E. Rothman, Joerg Bewersdorf
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ea973638e80347418c454d3d0a47cc6b
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spelling	oai:doaj.org-article:ea973638e80347418c454d3d0a47cc6b2021-12-02T16:56:45ZTwo-colour live-cell nanoscale imaging of intracellular targets10.1038/ncomms107782041-1723https://doaj.org/article/ea973638e80347418c454d3d0a47cc6b2016-03-01T00:00:00Zhttps://doi.org/10.1038/ncomms10778https://doaj.org/toc/2041-1723The intracellular applications of STED microscopy are limited by the availability of dyes. Here the authors develop a two-colour labelling strategy based on SiR and ATTO590 dyes, and apply their strategy to image various subcellular membrane compartments.Francesca BottanelliEmil B. KromannEdward S. AllgeyerRoman S. ErdmannStephanie Wood BaguleyGeorge SirinakisAlanna SchepartzDavid BaddeleyDerek K. ToomreJames E. RothmanJoerg BewersdorfNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-5 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Francesca Bottanelli Emil B. Kromann Edward S. Allgeyer Roman S. Erdmann Stephanie Wood Baguley George Sirinakis Alanna Schepartz David Baddeley Derek K. Toomre James E. Rothman Joerg Bewersdorf Two-colour live-cell nanoscale imaging of intracellular targets
description	The intracellular applications of STED microscopy are limited by the availability of dyes. Here the authors develop a two-colour labelling strategy based on SiR and ATTO590 dyes, and apply their strategy to image various subcellular membrane compartments.
format	article
author	Francesca Bottanelli Emil B. Kromann Edward S. Allgeyer Roman S. Erdmann Stephanie Wood Baguley George Sirinakis Alanna Schepartz David Baddeley Derek K. Toomre James E. Rothman Joerg Bewersdorf
author_facet	Francesca Bottanelli Emil B. Kromann Edward S. Allgeyer Roman S. Erdmann Stephanie Wood Baguley George Sirinakis Alanna Schepartz David Baddeley Derek K. Toomre James E. Rothman Joerg Bewersdorf
author_sort	Francesca Bottanelli
title	Two-colour live-cell nanoscale imaging of intracellular targets
title_short	Two-colour live-cell nanoscale imaging of intracellular targets
title_full	Two-colour live-cell nanoscale imaging of intracellular targets
title_fullStr	Two-colour live-cell nanoscale imaging of intracellular targets
title_full_unstemmed	Two-colour live-cell nanoscale imaging of intracellular targets
title_sort	two-colour live-cell nanoscale imaging of intracellular targets
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/ea973638e80347418c454d3d0a47cc6b
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Two-colour live-cell nanoscale imaging of intracellular targets

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