Evaluating the effect of Clostridium difficile conditioned medium on fecal microbiota community structure

Abstract Clostridium difficile infection (CDI) is typically associated with disturbed gut microbiota and changes related to decreased colonization resistance against C. difficile are well described. However, nothing is known about possible effects of C. difficile on gut microbiota restoration during...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sabina Horvat, Aleksander Mahnic, Martin Breskvar, Saso Dzeroski, Maja Rupnik
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eaa16ff3bd484cab85affe9033708e48
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:eaa16ff3bd484cab85affe9033708e48
record_format	dspace
spelling	oai:doaj.org-article:eaa16ff3bd484cab85affe9033708e482021-12-02T15:05:18ZEvaluating the effect of Clostridium difficile conditioned medium on fecal microbiota community structure10.1038/s41598-017-15434-12045-2322https://doaj.org/article/eaa16ff3bd484cab85affe9033708e482017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-15434-1https://doaj.org/toc/2045-2322Abstract Clostridium difficile infection (CDI) is typically associated with disturbed gut microbiota and changes related to decreased colonization resistance against C. difficile are well described. However, nothing is known about possible effects of C. difficile on gut microbiota restoration during or after CDI. In this study, we have mimicked such a situation by using C. difficile conditioned medium of six different C. difficile strains belonging to PCR ribotypes 027 and 014/020 for cultivation of fecal microbiota. A marked decrease of microbial diversity was observed in conditioned medium of both tested ribotypes. The majority of differences occurred within the phylum Firmicutes, with a general decrease of gut commensals with putative protective functions (i.e. Lactobacillus, Clostridium_XIVa) and an increase in opportunistic pathogens (i.e. Enterococcus). Bacterial populations in conditioned medium differed between the two C. difficile ribotypes, 027 and 014/020 and are likely associated with nutrient availability. Fecal microbiota cultivated in medium conditioned by E. coli, Salmonella Enteritidis or Staphylococcus epidermidis grouped together and was clearly different from microbiota cultivated in C. difficile conditioned medium suggesting that C. difficile effects are specific. Our results show that the changes observed in microbiota of CDI patients are partially directly influenced by C. difficile.Sabina HorvatAleksander MahnicMartin BreskvarSaso DzeroskiMaja RupnikNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sabina Horvat Aleksander Mahnic Martin Breskvar Saso Dzeroski Maja Rupnik Evaluating the effect of Clostridium difficile conditioned medium on fecal microbiota community structure
description	Abstract Clostridium difficile infection (CDI) is typically associated with disturbed gut microbiota and changes related to decreased colonization resistance against C. difficile are well described. However, nothing is known about possible effects of C. difficile on gut microbiota restoration during or after CDI. In this study, we have mimicked such a situation by using C. difficile conditioned medium of six different C. difficile strains belonging to PCR ribotypes 027 and 014/020 for cultivation of fecal microbiota. A marked decrease of microbial diversity was observed in conditioned medium of both tested ribotypes. The majority of differences occurred within the phylum Firmicutes, with a general decrease of gut commensals with putative protective functions (i.e. Lactobacillus, Clostridium_XIVa) and an increase in opportunistic pathogens (i.e. Enterococcus). Bacterial populations in conditioned medium differed between the two C. difficile ribotypes, 027 and 014/020 and are likely associated with nutrient availability. Fecal microbiota cultivated in medium conditioned by E. coli, Salmonella Enteritidis or Staphylococcus epidermidis grouped together and was clearly different from microbiota cultivated in C. difficile conditioned medium suggesting that C. difficile effects are specific. Our results show that the changes observed in microbiota of CDI patients are partially directly influenced by C. difficile.
format	article
author	Sabina Horvat Aleksander Mahnic Martin Breskvar Saso Dzeroski Maja Rupnik
author_facet	Sabina Horvat Aleksander Mahnic Martin Breskvar Saso Dzeroski Maja Rupnik
author_sort	Sabina Horvat
title	Evaluating the effect of Clostridium difficile conditioned medium on fecal microbiota community structure
title_short	Evaluating the effect of Clostridium difficile conditioned medium on fecal microbiota community structure
title_full	Evaluating the effect of Clostridium difficile conditioned medium on fecal microbiota community structure
title_fullStr	Evaluating the effect of Clostridium difficile conditioned medium on fecal microbiota community structure
title_full_unstemmed	Evaluating the effect of Clostridium difficile conditioned medium on fecal microbiota community structure
title_sort	evaluating the effect of clostridium difficile conditioned medium on fecal microbiota community structure
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/eaa16ff3bd484cab85affe9033708e48
work_keys_str_mv	AT sabinahorvat evaluatingtheeffectofclostridiumdifficileconditionedmediumonfecalmicrobiotacommunitystructure AT aleksandermahnic evaluatingtheeffectofclostridiumdifficileconditionedmediumonfecalmicrobiotacommunitystructure AT martinbreskvar evaluatingtheeffectofclostridiumdifficileconditionedmediumonfecalmicrobiotacommunitystructure AT sasodzeroski evaluatingtheeffectofclostridiumdifficileconditionedmediumonfecalmicrobiotacommunitystructure AT majarupnik evaluatingtheeffectofclostridiumdifficileconditionedmediumonfecalmicrobiotacommunitystructure
_version_	1718388928731414528

Evaluating the effect of Clostridium difficile conditioned medium on fecal microbiota community structure

Ejemplares similares