Synergistic Effects of Physicochemical Parameters on Bio-Fabrication of Mint Silver Nanoparticles: Structural Evaluation and Action Against HCT116 Colon Cancer Cells

Bilal Javed,1,2 Zia-ur-Rehman Mashwani2 1Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323, USA; 2Department of Botany, PMAS Arid Agriculture University, Rawalpindi, Punjab 46300, PakistanCorrespondence: Bilal Javed; Zia-ur-Reh...

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Autores principales:	Javed B, Mashwani ZR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	anticancer cell cycle analysis colorectal cancer green synthesis mentha arvensis reaction dynamics. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/eaa51f4b4a9647a892c7487b97f5035a
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spelling	oai:doaj.org-article:eaa51f4b4a9647a892c7487b97f5035a2021-12-02T12:00:36ZSynergistic Effects of Physicochemical Parameters on Bio-Fabrication of Mint Silver Nanoparticles: Structural Evaluation and Action Against HCT116 Colon Cancer Cells1178-2013https://doaj.org/article/eaa51f4b4a9647a892c7487b97f5035a2020-05-01T00:00:00Zhttps://www.dovepress.com/synergistic-effects-of-physicochemical-parameters-on-bio-fabrication-o-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Bilal Javed,1,2 Zia-ur-Rehman Mashwani2 1Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323, USA; 2Department of Botany, PMAS Arid Agriculture University, Rawalpindi, Punjab 46300, PakistanCorrespondence: Bilal Javed; Zia-ur-Rehman MashwaniDepartment of Botany, PMAS Arid Agriculture University, Rawalpindi, Punjab 46300, PakistanEmail javedbilal87@gmail.com; zia.botany@gmail.comBackground: Physicochemical parameters such as temperature, pH, the concentration of the AgNO3 and ratio of reactants act synergistically to influence the reaction kinetics, molecular mechanics, enzymatic catalysis and protein conformations that aid to affect the size, shape and biochemical corona of nanoparticles. The present study was performed to investigate the influence of reaction parameters on the bio-fabrication of silver nanoparticles (AgNPs) by using Mentha arvensis and to determine their potential to control the proliferation of colon cancer cells’.Methods: Plant-mediated method was used for the bio-fabrication and stabilization of AgNPs. Reaction parameters were arranged, and surface plasmon resonance (SPR) bands of AgNPs were collected by using a UV-Visible spectrophotometer. NPs were characterized structurally and optically by using SEM, AFM, EDX and DLS techniques. AgNPs and plant aqueous extract were tested against HCT116 colon cancer cells by using SRB assay, Annexin V assay and cell cycle analysis.Results: Spectrophotometric comparison of various reaction conditions manifested that 5 mM of AgNO3, 60 °C in an acidic pH and a mixing ratio of 1:9 of plant extract and AgNO3, respectively, are the optimized conditions for AgNP synthesis. Structural evaluation by SEM, AFM and particle size analysis confirmed that the NPs are < 100 nm and are anisotropic, spherical, triangular and moderately dispersed in the colloidal mixture. SRB assay expressed biomass-stabilized AgNPs as effective cytotoxic particles against HCT116 colon cancer cells, and the IC50 was measured at 1.7 μg/mL. Annexin V apoptosis assay further confirmed that the AgNPs induce apoptosis in a dose-dependent manner. Experimental evidence manifested that the AgNPs arrest cell cycle and expressed entrapment of a greater number of cells in the Sub-G1 phase, further verifying the anticancer abilities of AgNPs.Conclusion: These findings explain the synergistic effects of physicochemical parameters to optimize the phytosynthesis of biocompatible AgNPs to overcome the limitations of conventional chemotherapeutic treatments of colon cancer cells.Keywords: anticancer, cell cycle analysis, colorectal cancer, green synthesis, Mentha arvensis, reaction dynamicsJaved BMashwani ZRDove Medical Pressarticleanticancercell cycle analysiscolorectal cancergreen synthesismentha arvensisreaction dynamics.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 3621-3637 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	anticancer cell cycle analysis colorectal cancer green synthesis mentha arvensis reaction dynamics. Medicine (General) R5-920
spellingShingle	anticancer cell cycle analysis colorectal cancer green synthesis mentha arvensis reaction dynamics. Medicine (General) R5-920 Javed B Mashwani ZR Synergistic Effects of Physicochemical Parameters on Bio-Fabrication of Mint Silver Nanoparticles: Structural Evaluation and Action Against HCT116 Colon Cancer Cells
description	Bilal Javed,1,2 Zia-ur-Rehman Mashwani2 1Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323, USA; 2Department of Botany, PMAS Arid Agriculture University, Rawalpindi, Punjab 46300, PakistanCorrespondence: Bilal Javed; Zia-ur-Rehman MashwaniDepartment of Botany, PMAS Arid Agriculture University, Rawalpindi, Punjab 46300, PakistanEmail javedbilal87@gmail.com; zia.botany@gmail.comBackground: Physicochemical parameters such as temperature, pH, the concentration of the AgNO3 and ratio of reactants act synergistically to influence the reaction kinetics, molecular mechanics, enzymatic catalysis and protein conformations that aid to affect the size, shape and biochemical corona of nanoparticles. The present study was performed to investigate the influence of reaction parameters on the bio-fabrication of silver nanoparticles (AgNPs) by using Mentha arvensis and to determine their potential to control the proliferation of colon cancer cells’.Methods: Plant-mediated method was used for the bio-fabrication and stabilization of AgNPs. Reaction parameters were arranged, and surface plasmon resonance (SPR) bands of AgNPs were collected by using a UV-Visible spectrophotometer. NPs were characterized structurally and optically by using SEM, AFM, EDX and DLS techniques. AgNPs and plant aqueous extract were tested against HCT116 colon cancer cells by using SRB assay, Annexin V assay and cell cycle analysis.Results: Spectrophotometric comparison of various reaction conditions manifested that 5 mM of AgNO3, 60 °C in an acidic pH and a mixing ratio of 1:9 of plant extract and AgNO3, respectively, are the optimized conditions for AgNP synthesis. Structural evaluation by SEM, AFM and particle size analysis confirmed that the NPs are < 100 nm and are anisotropic, spherical, triangular and moderately dispersed in the colloidal mixture. SRB assay expressed biomass-stabilized AgNPs as effective cytotoxic particles against HCT116 colon cancer cells, and the IC50 was measured at 1.7 μg/mL. Annexin V apoptosis assay further confirmed that the AgNPs induce apoptosis in a dose-dependent manner. Experimental evidence manifested that the AgNPs arrest cell cycle and expressed entrapment of a greater number of cells in the Sub-G1 phase, further verifying the anticancer abilities of AgNPs.Conclusion: These findings explain the synergistic effects of physicochemical parameters to optimize the phytosynthesis of biocompatible AgNPs to overcome the limitations of conventional chemotherapeutic treatments of colon cancer cells.Keywords: anticancer, cell cycle analysis, colorectal cancer, green synthesis, Mentha arvensis, reaction dynamics
format	article
author	Javed B Mashwani ZR
author_facet	Javed B Mashwani ZR
author_sort	Javed B
title	Synergistic Effects of Physicochemical Parameters on Bio-Fabrication of Mint Silver Nanoparticles: Structural Evaluation and Action Against HCT116 Colon Cancer Cells
title_short	Synergistic Effects of Physicochemical Parameters on Bio-Fabrication of Mint Silver Nanoparticles: Structural Evaluation and Action Against HCT116 Colon Cancer Cells
title_full	Synergistic Effects of Physicochemical Parameters on Bio-Fabrication of Mint Silver Nanoparticles: Structural Evaluation and Action Against HCT116 Colon Cancer Cells
title_fullStr	Synergistic Effects of Physicochemical Parameters on Bio-Fabrication of Mint Silver Nanoparticles: Structural Evaluation and Action Against HCT116 Colon Cancer Cells
title_full_unstemmed	Synergistic Effects of Physicochemical Parameters on Bio-Fabrication of Mint Silver Nanoparticles: Structural Evaluation and Action Against HCT116 Colon Cancer Cells
title_sort	synergistic effects of physicochemical parameters on bio-fabrication of mint silver nanoparticles: structural evaluation and action against hct116 colon cancer cells
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/eaa51f4b4a9647a892c7487b97f5035a
work_keys_str_mv	AT javedb synergisticeffectsofphysicochemicalparametersonbiofabricationofmintsilvernanoparticlesstructuralevaluationandactionagainsthct116coloncancercells AT mashwanizr synergisticeffectsofphysicochemicalparametersonbiofabricationofmintsilvernanoparticlesstructuralevaluationandactionagainsthct116coloncancercells
_version_	1718394782642864128

Synergistic Effects of Physicochemical Parameters on Bio-Fabrication of Mint Silver Nanoparticles: Structural Evaluation and Action Against HCT116 Colon Cancer Cells

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