Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors

The determination of Human Chorionic Gonadotropin (hCG) and Alpha Fetoprotein (AFP) levels on serum and amniotic fluid plays a fundamental role in the diagnosis and follow-up of specific physiological or pathological conditions (e.g., pregnancy, threat of abortion or germ cell tumors). Recently, the...

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Autores principales:	Annamaria D'Alessandro, Domenico Ciavardelli, Anna Pastore, Germana Giannone, Giada Del Baldo, Andrea Carai, Angela Mastronuzzi, Andrea Onetti Muda, Ottavia Porzio
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	AFP hCG cerebrospinal fluid germ cell tumors central nervous system tumors Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/eaa7a5fb9003413fb1b4ac9128126353
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spelling	oai:doaj.org-article:eaa7a5fb9003413fb1b4ac91281263532021-11-25T17:20:27ZCerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors10.3390/diagnostics111119802075-4418https://doaj.org/article/eaa7a5fb9003413fb1b4ac91281263532021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/1980https://doaj.org/toc/2075-4418The determination of Human Chorionic Gonadotropin (hCG) and Alpha Fetoprotein (AFP) levels on serum and amniotic fluid plays a fundamental role in the diagnosis and follow-up of specific physiological or pathological conditions (e.g., pregnancy, threat of abortion or germ cell tumors). Recently, the quantification of hCG and AFP in other biological fluids has gained great attention to support the diagnosis, prognosis and follow-up of neoplastic diseases deriving from trophoblastic cells, such as germinomas. Most of the commercial kits for hCG and AFP assays are developed to be used on biological fluids such as serum/plasma and/or urine by manufacturing companies. The aim of this work was to evaluate the suitability of the analytical method certified for the use on serum, and/or amniotic fluid for the quantification of hCG and AFP in cerebrospinal fluid, carrying out an internal validation protocol. The data reported here show that the automated immunochemical method is fit for quantification of hCG and AFP in cerebrospinal fluid (CSF), allowing selective and specific diagnosis of secreting germ cell tumors. This is confirmed by the positive correlation between elevated levels of hCG or AFP and the diagnosis of brain tumors.Annamaria D'AlessandroDomenico CiavardelliAnna PastoreGermana GiannoneGiada Del BaldoAndrea CaraiAngela MastronuzziAndrea Onetti MudaOttavia PorzioMDPI AGarticleAFPhCGcerebrospinal fluidgerm cell tumorscentral nervous system tumorsMedicine (General)R5-920ENDiagnostics, Vol 11, Iss 1980, p 1980 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	AFP hCG cerebrospinal fluid germ cell tumors central nervous system tumors Medicine (General) R5-920
spellingShingle	AFP hCG cerebrospinal fluid germ cell tumors central nervous system tumors Medicine (General) R5-920 Annamaria D'Alessandro Domenico Ciavardelli Anna Pastore Germana Giannone Giada Del Baldo Andrea Carai Angela Mastronuzzi Andrea Onetti Muda Ottavia Porzio Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors
description	The determination of Human Chorionic Gonadotropin (hCG) and Alpha Fetoprotein (AFP) levels on serum and amniotic fluid plays a fundamental role in the diagnosis and follow-up of specific physiological or pathological conditions (e.g., pregnancy, threat of abortion or germ cell tumors). Recently, the quantification of hCG and AFP in other biological fluids has gained great attention to support the diagnosis, prognosis and follow-up of neoplastic diseases deriving from trophoblastic cells, such as germinomas. Most of the commercial kits for hCG and AFP assays are developed to be used on biological fluids such as serum/plasma and/or urine by manufacturing companies. The aim of this work was to evaluate the suitability of the analytical method certified for the use on serum, and/or amniotic fluid for the quantification of hCG and AFP in cerebrospinal fluid, carrying out an internal validation protocol. The data reported here show that the automated immunochemical method is fit for quantification of hCG and AFP in cerebrospinal fluid (CSF), allowing selective and specific diagnosis of secreting germ cell tumors. This is confirmed by the positive correlation between elevated levels of hCG or AFP and the diagnosis of brain tumors.
format	article
author	Annamaria D'Alessandro Domenico Ciavardelli Anna Pastore Germana Giannone Giada Del Baldo Andrea Carai Angela Mastronuzzi Andrea Onetti Muda Ottavia Porzio
author_facet	Annamaria D'Alessandro Domenico Ciavardelli Anna Pastore Germana Giannone Giada Del Baldo Andrea Carai Angela Mastronuzzi Andrea Onetti Muda Ottavia Porzio
author_sort	Annamaria D'Alessandro
title	Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors
title_short	Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors
title_full	Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors
title_fullStr	Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors
title_full_unstemmed	Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors
title_sort	cerebrospinal fluid levels of afp and hcg: validation of the analytical method and application in the diagnosis of central nervous system germ cell tumors
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/eaa7a5fb9003413fb1b4ac9128126353
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Cerebrospinal Fluid Levels of AFP and hCG: Validation of the Analytical Method and Application in the Diagnosis of Central Nervous System Germ Cell Tumors

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