Impact of Low Interferon-γ and IL-10 Levels on TNF-α and IL-6 Production by PHA-Induced PBMCs in Type 2 Diabetes Mellitus
Rona Kartika,1 Dyah Purnamasari,2 Saraswati Pradipta,1 Rahma A Larasati,3 Heri Wibowo4 1Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia; 2Division of Metabolic Endocrinology and Diabetes, Department of Internal Medicine, Faculty of Medicine...
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Dove Medical Press
2020
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oai:doaj.org-article:eaa7bb82c51545469cdc1a42fd7def952021-12-02T09:54:14ZImpact of Low Interferon-γ and IL-10 Levels on TNF-α and IL-6 Production by PHA-Induced PBMCs in Type 2 Diabetes Mellitus1178-7031https://doaj.org/article/eaa7bb82c51545469cdc1a42fd7def952020-04-01T00:00:00Zhttps://www.dovepress.com/impact-of-low-interferon-gamma-and-il-10-levels-on-tnf-alpha-and-il-6--peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Rona Kartika,1 Dyah Purnamasari,2 Saraswati Pradipta,1 Rahma A Larasati,3 Heri Wibowo4 1Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia; 2Division of Metabolic Endocrinology and Diabetes, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia; 3Department of Biomedicines, Faculty of Medicine, Universitas Muhammadiyah Jakarta, Jakarta, Indonesia; 4Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaCorrespondence: Heri WibowoDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaTel +62-812-8328-1905Email bowoheri04@gmail.comPurpose: In this study, we analyzed the production of interferon γ (IFN-γ) and interleukin 10 (IL-10) by peripheral blood mononuclear cells (PBMCs) in patients with type 2 diabetes mellitus (T2DM). We aimed to investigate the capacity of monocytes to produce tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) following IFN-γ stimulation and the associated role of IL-10 in TNF-α and IL-6 production.Patients and Methods: In vitro experiments were conducted on PBMCs obtained from 19 patients with T2DM and 17 healthy participants. PBMCs were isolated from venous blood by density gradient centrifugation, followed by 3-day phytohemagglutinin induction. In vitro production of TNF-α, IL-6, IFN-γ, and IL-10 was measured using the multiplex immunoassay. Statistical analysis was performed using IBM SPSS 23 version.Results: IFN-γ concentration in the T2DM group was significantly lower than that in control group (T2DM 7,700.86 ± 3,037.77 vs control 10,672.69 ± 5,625.50 pg/mL; p = 0.048). However, TNF-α, IL-6, and IL-10 levels showed no significant difference between the two groups. The TNF-α/IFN-γ and IL-6/IFN-γ ratios were significantly higher in T2DM than in the control group (p = 0.026 and p = 0.048, respectively). In T2DM, the high TNF-α/IFN-γ ratio was consistent, with the low baseline IL-10 level (p = 0.022).Conclusion: In T2DM, T-cell response is impaired with significantly reduced IFN-γ production, and simultaneously, circulatory monocytes show enhanced cellular responsiveness to inflammatory stimuli. The low baseline IL-10 level likely contributes to such an immune response.Keywords: type 2 diabetes mellitus, proinflammatory response, cytokinesKartika RPurnamasari DPradipta SLarasati RAWibowo HDove Medical Pressarticletype 2 diabetes mellitusproinflammatory responsecytokinesPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 13, Pp 187-193 (2020) |
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type 2 diabetes mellitus proinflammatory response cytokines Pathology RB1-214 Therapeutics. Pharmacology RM1-950 |
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type 2 diabetes mellitus proinflammatory response cytokines Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Kartika R Purnamasari D Pradipta S Larasati RA Wibowo H Impact of Low Interferon-γ and IL-10 Levels on TNF-α and IL-6 Production by PHA-Induced PBMCs in Type 2 Diabetes Mellitus |
| description |
Rona Kartika,1 Dyah Purnamasari,2 Saraswati Pradipta,1 Rahma A Larasati,3 Heri Wibowo4 1Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia; 2Division of Metabolic Endocrinology and Diabetes, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia; 3Department of Biomedicines, Faculty of Medicine, Universitas Muhammadiyah Jakarta, Jakarta, Indonesia; 4Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaCorrespondence: Heri WibowoDepartment of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaTel +62-812-8328-1905Email bowoheri04@gmail.comPurpose: In this study, we analyzed the production of interferon γ (IFN-γ) and interleukin 10 (IL-10) by peripheral blood mononuclear cells (PBMCs) in patients with type 2 diabetes mellitus (T2DM). We aimed to investigate the capacity of monocytes to produce tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) following IFN-γ stimulation and the associated role of IL-10 in TNF-α and IL-6 production.Patients and Methods: In vitro experiments were conducted on PBMCs obtained from 19 patients with T2DM and 17 healthy participants. PBMCs were isolated from venous blood by density gradient centrifugation, followed by 3-day phytohemagglutinin induction. In vitro production of TNF-α, IL-6, IFN-γ, and IL-10 was measured using the multiplex immunoassay. Statistical analysis was performed using IBM SPSS 23 version.Results: IFN-γ concentration in the T2DM group was significantly lower than that in control group (T2DM 7,700.86 ± 3,037.77 vs control 10,672.69 ± 5,625.50 pg/mL; p = 0.048). However, TNF-α, IL-6, and IL-10 levels showed no significant difference between the two groups. The TNF-α/IFN-γ and IL-6/IFN-γ ratios were significantly higher in T2DM than in the control group (p = 0.026 and p = 0.048, respectively). In T2DM, the high TNF-α/IFN-γ ratio was consistent, with the low baseline IL-10 level (p = 0.022).Conclusion: In T2DM, T-cell response is impaired with significantly reduced IFN-γ production, and simultaneously, circulatory monocytes show enhanced cellular responsiveness to inflammatory stimuli. The low baseline IL-10 level likely contributes to such an immune response.Keywords: type 2 diabetes mellitus, proinflammatory response, cytokines |
| format |
article |
| author |
Kartika R Purnamasari D Pradipta S Larasati RA Wibowo H |
| author_facet |
Kartika R Purnamasari D Pradipta S Larasati RA Wibowo H |
| author_sort |
Kartika R |
| title |
Impact of Low Interferon-γ and IL-10 Levels on TNF-α and IL-6 Production by PHA-Induced PBMCs in Type 2 Diabetes Mellitus |
| title_short |
Impact of Low Interferon-γ and IL-10 Levels on TNF-α and IL-6 Production by PHA-Induced PBMCs in Type 2 Diabetes Mellitus |
| title_full |
Impact of Low Interferon-γ and IL-10 Levels on TNF-α and IL-6 Production by PHA-Induced PBMCs in Type 2 Diabetes Mellitus |
| title_fullStr |
Impact of Low Interferon-γ and IL-10 Levels on TNF-α and IL-6 Production by PHA-Induced PBMCs in Type 2 Diabetes Mellitus |
| title_full_unstemmed |
Impact of Low Interferon-γ and IL-10 Levels on TNF-α and IL-6 Production by PHA-Induced PBMCs in Type 2 Diabetes Mellitus |
| title_sort |
impact of low interferon-γ and il-10 levels on tnf-α and il-6 production by pha-induced pbmcs in type 2 diabetes mellitus |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/eaa7bb82c51545469cdc1a42fd7def95 |
| work_keys_str_mv |
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