The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells

Background. The extract of freshwater clams has been used to protect the body against liver diseases in traditional folk medicine. This study aims at investigating the effects of freshwater clam extract on activated hepatic stellate cells (aHSCs), which are critical contributors to liver fibrosis. M...

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Autores principales:	Shou-Lun Lee, Wei-Hsiang Hsu, Chia-Ming Tu, Wen-Han Wang, Cheng-Yao Yang, Hsien-Kuang Lee, Ting-Yu Chin
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/eaa9089553c242b2923fa2d55ba3ac7d
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spelling	oai:doaj.org-article:eaa9089553c242b2923fa2d55ba3ac7d2021-11-22T01:10:20ZThe Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells1741-428810.1155/2021/6065168https://doaj.org/article/eaa9089553c242b2923fa2d55ba3ac7d2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/6065168https://doaj.org/toc/1741-4288Background. The extract of freshwater clams has been used to protect the body against liver diseases in traditional folk medicine. This study aims at investigating the effects of freshwater clam extract on activated hepatic stellate cells (aHSCs), which are critical contributors to liver fibrosis. Methods. The aHSCs used in this study were derived from hepatic stellate cells that were isolated and purified from the livers of male Wistar rats and then transformed into the activated phenotype by culturing on uncoated plastic dishes. Freshwater clam extract (CE) was collected after the outflow from the live freshwater clams in a water bath at 100°C for 60 min. The effects of CE on aHSCs were analyzed by MTT assay, flow cytometry, Oil Red O (ORO) staining, western blot, and real-time RT-PCR. Results. The results indicated that CE suppressed the proliferation of aHSCs through G0/G1 cell cycle arrest by downregulating cyclin D1 and upregulating p27. The expression levels of a-SMA, collagen I, TGF-β, and TNF-α were inhibited in the CE-treated aHSCs. In addition, the CE treatment increased the lipid contents in aHSCs by promoting PPARγ expression. Furthermore, CE modulated the expression of ECM-related genes, i.e., by upregulating MMP-9 and downregulating TIMP-II. Conclusions. These data revealed that CE could induce the deactivation of aHSCs. We therefore suggest that CE has potential as an adjuvant therapeutic agent against hepatic fibrosis.Shou-Lun LeeWei-Hsiang HsuChia-Ming TuWen-Han WangCheng-Yao YangHsien-Kuang LeeTing-Yu ChinHindawi LimitedarticleOther systems of medicineRZ201-999ENEvidence-Based Complementary and Alternative Medicine, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Other systems of medicine RZ201-999
spellingShingle	Other systems of medicine RZ201-999 Shou-Lun Lee Wei-Hsiang Hsu Chia-Ming Tu Wen-Han Wang Cheng-Yao Yang Hsien-Kuang Lee Ting-Yu Chin The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells
description	Background. The extract of freshwater clams has been used to protect the body against liver diseases in traditional folk medicine. This study aims at investigating the effects of freshwater clam extract on activated hepatic stellate cells (aHSCs), which are critical contributors to liver fibrosis. Methods. The aHSCs used in this study were derived from hepatic stellate cells that were isolated and purified from the livers of male Wistar rats and then transformed into the activated phenotype by culturing on uncoated plastic dishes. Freshwater clam extract (CE) was collected after the outflow from the live freshwater clams in a water bath at 100°C for 60 min. The effects of CE on aHSCs were analyzed by MTT assay, flow cytometry, Oil Red O (ORO) staining, western blot, and real-time RT-PCR. Results. The results indicated that CE suppressed the proliferation of aHSCs through G0/G1 cell cycle arrest by downregulating cyclin D1 and upregulating p27. The expression levels of a-SMA, collagen I, TGF-β, and TNF-α were inhibited in the CE-treated aHSCs. In addition, the CE treatment increased the lipid contents in aHSCs by promoting PPARγ expression. Furthermore, CE modulated the expression of ECM-related genes, i.e., by upregulating MMP-9 and downregulating TIMP-II. Conclusions. These data revealed that CE could induce the deactivation of aHSCs. We therefore suggest that CE has potential as an adjuvant therapeutic agent against hepatic fibrosis.
format	article
author	Shou-Lun Lee Wei-Hsiang Hsu Chia-Ming Tu Wen-Han Wang Cheng-Yao Yang Hsien-Kuang Lee Ting-Yu Chin
author_facet	Shou-Lun Lee Wei-Hsiang Hsu Chia-Ming Tu Wen-Han Wang Cheng-Yao Yang Hsien-Kuang Lee Ting-Yu Chin
author_sort	Shou-Lun Lee
title	The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells
title_short	The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells
title_full	The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells
title_fullStr	The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells
title_full_unstemmed	The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells
title_sort	effects of freshwater clam (corbicula fluminea) extract on activated hepatic stellate cells
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/eaa9089553c242b2923fa2d55ba3ac7d
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The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells

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