Evaluation of Nitric Oxide production by monocytes in children with acute lymphoblastic leukemia
Objective: Nitric Oxide (NO) is produced by different cells such as monocytes and plays an important role in non-specific immunity. In addition to its numerous physiological and pathophysiological effects, (NO) has tumoricidal activity. It seems patients with acute lymphoblastic leukemia (ALL) have...
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| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | EN FA |
| Publicado: |
Babol University of Medical Sciences
2001
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/eaab7c7f99e8407ca3329773230b2070 |
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| Sumario: | Objective: Nitric Oxide (NO) is produced by different cells such as monocytes and plays an important role in non-specific immunity. In addition to its numerous physiological and pathophysiological effects, (NO) has tumoricidal activity. It seems patients with acute lymphoblastic leukemia (ALL) have an imbalance and a defect in their immune system, especially in (NO) production. Therefore, in this study (NO) production by monocytes in children with (ALL) was evaluated before and after treatment and the results were compared to an age-matched reference population. Methods: Peripheral blood samples from 30 patients with (ALL) were collected before treatment and after induction of remission. Samples were also taken from 30 healthy children. Mononuclear cells and then monocytes were separated from blood samples and cultured in the presence of activators and inhibitors of (NO) pathway. For evaluation of (NO) production, nitrite level, which is a determinant of (NO) output, was measured in supernatants of culture media by the Griess method. Findings: The results show that the level of (NO) production before treatment in comparison to control group is significantly increased. Conclusion: Although there are cellular immunity defects in patients with (ALL), it seems (NO) production in a non-specific pathway by monocytes is not altered and may have anti-tumoral activity in these patients. |
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