Evaluation of Nitric Oxide production by monocytes in children with acute lymphoblastic leukemia

Objective: Nitric Oxide (NO) is produced by different cells such as monocytes and plays an important role in non-specific immunity. In addition to its numerous physiological and pathophysiological effects, (NO) has tumoricidal activity. It seems patients with acute lymphoblastic leukemia (ALL) have...

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Autores principales:	R Chegeni, A Zavaran Hosseini, AA Pour Fathollah, P Vosough
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2001
Materias:	nitric oxide monocytes acute lymphoblastic leukemia Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/eaab7c7f99e8407ca3329773230b2070
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id	oai:doaj.org-article:eaab7c7f99e8407ca3329773230b2070
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spelling	oai:doaj.org-article:eaab7c7f99e8407ca3329773230b20702021-11-10T09:20:53ZEvaluation of Nitric Oxide production by monocytes in children with acute lymphoblastic leukemia1561-41072251-7170https://doaj.org/article/eaab7c7f99e8407ca3329773230b20702001-01-01T00:00:00Zhttp://jbums.org/article-1-2879-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170Objective: Nitric Oxide (NO) is produced by different cells such as monocytes and plays an important role in non-specific immunity. In addition to its numerous physiological and pathophysiological effects, (NO) has tumoricidal activity. It seems patients with acute lymphoblastic leukemia (ALL) have an imbalance and a defect in their immune system, especially in (NO) production. Therefore, in this study (NO) production by monocytes in children with (ALL) was evaluated before and after treatment and the results were compared to an age-matched reference population. Methods: Peripheral blood samples from 30 patients with (ALL) were collected before treatment and after induction of remission. Samples were also taken from 30 healthy children. Mononuclear cells and then monocytes were separated from blood samples and cultured in the presence of activators and inhibitors of (NO) pathway. For evaluation of (NO) production, nitrite level, which is a determinant of (NO) output, was measured in supernatants of culture media by the Griess method. Findings: The results show that the level of (NO) production before treatment in comparison to control group is significantly increased. Conclusion: Although there are cellular immunity defects in patients with (ALL), it seems (NO) production in a non-specific pathway by monocytes is not altered and may have anti-tumoral activity in these patients.R ChegeniA Zavaran HosseiniAA Pour FathollahP VosoughBabol University of Medical Sciencesarticlenitric oxidemonocytesacute lymphoblastic leukemiaMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 3, Iss 1, Pp 8-13 (2001)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	nitric oxide monocytes acute lymphoblastic leukemia Medicine R Medicine (General) R5-920
spellingShingle	nitric oxide monocytes acute lymphoblastic leukemia Medicine R Medicine (General) R5-920 R Chegeni A Zavaran Hosseini AA Pour Fathollah P Vosough Evaluation of Nitric Oxide production by monocytes in children with acute lymphoblastic leukemia
description	Objective: Nitric Oxide (NO) is produced by different cells such as monocytes and plays an important role in non-specific immunity. In addition to its numerous physiological and pathophysiological effects, (NO) has tumoricidal activity. It seems patients with acute lymphoblastic leukemia (ALL) have an imbalance and a defect in their immune system, especially in (NO) production. Therefore, in this study (NO) production by monocytes in children with (ALL) was evaluated before and after treatment and the results were compared to an age-matched reference population. Methods: Peripheral blood samples from 30 patients with (ALL) were collected before treatment and after induction of remission. Samples were also taken from 30 healthy children. Mononuclear cells and then monocytes were separated from blood samples and cultured in the presence of activators and inhibitors of (NO) pathway. For evaluation of (NO) production, nitrite level, which is a determinant of (NO) output, was measured in supernatants of culture media by the Griess method. Findings: The results show that the level of (NO) production before treatment in comparison to control group is significantly increased. Conclusion: Although there are cellular immunity defects in patients with (ALL), it seems (NO) production in a non-specific pathway by monocytes is not altered and may have anti-tumoral activity in these patients.
format	article
author	R Chegeni A Zavaran Hosseini AA Pour Fathollah P Vosough
author_facet	R Chegeni A Zavaran Hosseini AA Pour Fathollah P Vosough
author_sort	R Chegeni
title	Evaluation of Nitric Oxide production by monocytes in children with acute lymphoblastic leukemia
title_short	Evaluation of Nitric Oxide production by monocytes in children with acute lymphoblastic leukemia
title_full	Evaluation of Nitric Oxide production by monocytes in children with acute lymphoblastic leukemia
title_fullStr	Evaluation of Nitric Oxide production by monocytes in children with acute lymphoblastic leukemia
title_full_unstemmed	Evaluation of Nitric Oxide production by monocytes in children with acute lymphoblastic leukemia
title_sort	evaluation of nitric oxide production by monocytes in children with acute lymphoblastic leukemia
publisher	Babol University of Medical Sciences
publishDate	2001
url	https://doaj.org/article/eaab7c7f99e8407ca3329773230b2070
work_keys_str_mv	AT rchegeni evaluationofnitricoxideproductionbymonocytesinchildrenwithacutelymphoblasticleukemia AT azavaranhosseini evaluationofnitricoxideproductionbymonocytesinchildrenwithacutelymphoblasticleukemia AT aapourfathollah evaluationofnitricoxideproductionbymonocytesinchildrenwithacutelymphoblasticleukemia AT pvosough evaluationofnitricoxideproductionbymonocytesinchildrenwithacutelymphoblasticleukemia
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Evaluation of Nitric Oxide production by monocytes in children with acute lymphoblastic leukemia

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